Ampelopsin Inhibits H 2 O 2 ‐induced Apoptosis by ERK and Akt Signaling Pathways and Up‐regulation of Heme Oxygenase‐1
Oxidative stress plays an important role in neurodegenerative disorders. Ampelopsin, a flavonoid abundant in Rattan tea ( Ampelopsis grossedentata ), is a potent antioxidant and its neuroprotective effect against H 2 O 2 ‐induced apoptosis in PC12 cells is investigated here for the first time. It wa...
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| Veröffentlicht in: | Phytotherapy research 2012-07, Vol.26 (7), p.988-994 |
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| container_title | Phytotherapy research |
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| creator | Kou, Xianjuan Shen, Keyin An, Yuhui Qi, Shimei Dai, Wu‐Xing Yin, Zhimin |
| description | Oxidative stress plays an important role in neurodegenerative disorders. Ampelopsin, a flavonoid abundant in Rattan tea (
Ampelopsis grossedentata
), is a potent antioxidant and its neuroprotective effect against H
2
O
2
‐induced apoptosis in PC12 cells is investigated here for the first time. It was found that treatment of cells with ampelopsin for 1 h significantly reduced the loss of vitality, LDH release and apoptosis and inhibited the formation of reactive oxygen species (ROS). Ampelopsin was able to prevent the activation of p38 induced by H
2
O
2
. In addition, up‐regulation of heme oxygenase‐1 (HO‐1) expression by ampelopsin was shown to be both dose‐ and time‐dependent. Mechanically, HO‐1 expression induced by ampelopsin was found to be due to activation of the ERK and Akt signaling pathways, because it was almost completely blocked by the specific inhibitors of ERK and Akt. These results suggest that ampelopsin increases cellular antioxidant defense through activation of the ERK and Akt signaling pathways, which induces HO‐1 expression and thereby protects PC12 cells from H
2
O
2
‐induced apoptosis. Copyright © 2011 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/ptr.3671 |
| format | Article |
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Ampelopsis grossedentata
), is a potent antioxidant and its neuroprotective effect against H
2
O
2
‐induced apoptosis in PC12 cells is investigated here for the first time. It was found that treatment of cells with ampelopsin for 1 h significantly reduced the loss of vitality, LDH release and apoptosis and inhibited the formation of reactive oxygen species (ROS). Ampelopsin was able to prevent the activation of p38 induced by H
2
O
2
. In addition, up‐regulation of heme oxygenase‐1 (HO‐1) expression by ampelopsin was shown to be both dose‐ and time‐dependent. Mechanically, HO‐1 expression induced by ampelopsin was found to be due to activation of the ERK and Akt signaling pathways, because it was almost completely blocked by the specific inhibitors of ERK and Akt. These results suggest that ampelopsin increases cellular antioxidant defense through activation of the ERK and Akt signaling pathways, which induces HO‐1 expression and thereby protects PC12 cells from H
2
O
2
‐induced apoptosis. Copyright © 2011 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.3671</identifier><language>eng</language><ispartof>Phytotherapy research, 2012-07, Vol.26 (7), p.988-994</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c721-145146b52089e07d55f13692ec2c2b4c32fde92a4c9e04b3e8458d74e20818ea3</citedby><cites>FETCH-LOGICAL-c721-145146b52089e07d55f13692ec2c2b4c32fde92a4c9e04b3e8458d74e20818ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Kou, Xianjuan</creatorcontrib><creatorcontrib>Shen, Keyin</creatorcontrib><creatorcontrib>An, Yuhui</creatorcontrib><creatorcontrib>Qi, Shimei</creatorcontrib><creatorcontrib>Dai, Wu‐Xing</creatorcontrib><creatorcontrib>Yin, Zhimin</creatorcontrib><title>Ampelopsin Inhibits H 2 O 2 ‐induced Apoptosis by ERK and Akt Signaling Pathways and Up‐regulation of Heme Oxygenase‐1</title><title>Phytotherapy research</title><description>Oxidative stress plays an important role in neurodegenerative disorders. Ampelopsin, a flavonoid abundant in Rattan tea (
Ampelopsis grossedentata
), is a potent antioxidant and its neuroprotective effect against H
2
O
2
‐induced apoptosis in PC12 cells is investigated here for the first time. It was found that treatment of cells with ampelopsin for 1 h significantly reduced the loss of vitality, LDH release and apoptosis and inhibited the formation of reactive oxygen species (ROS). Ampelopsin was able to prevent the activation of p38 induced by H
2
O
2
. In addition, up‐regulation of heme oxygenase‐1 (HO‐1) expression by ampelopsin was shown to be both dose‐ and time‐dependent. Mechanically, HO‐1 expression induced by ampelopsin was found to be due to activation of the ERK and Akt signaling pathways, because it was almost completely blocked by the specific inhibitors of ERK and Akt. These results suggest that ampelopsin increases cellular antioxidant defense through activation of the ERK and Akt signaling pathways, which induces HO‐1 expression and thereby protects PC12 cells from H
2
O
2
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Ampelopsis grossedentata
), is a potent antioxidant and its neuroprotective effect against H
2
O
2
‐induced apoptosis in PC12 cells is investigated here for the first time. It was found that treatment of cells with ampelopsin for 1 h significantly reduced the loss of vitality, LDH release and apoptosis and inhibited the formation of reactive oxygen species (ROS). Ampelopsin was able to prevent the activation of p38 induced by H
2
O
2
. In addition, up‐regulation of heme oxygenase‐1 (HO‐1) expression by ampelopsin was shown to be both dose‐ and time‐dependent. Mechanically, HO‐1 expression induced by ampelopsin was found to be due to activation of the ERK and Akt signaling pathways, because it was almost completely blocked by the specific inhibitors of ERK and Akt. These results suggest that ampelopsin increases cellular antioxidant defense through activation of the ERK and Akt signaling pathways, which induces HO‐1 expression and thereby protects PC12 cells from H
2
O
2
‐induced apoptosis. Copyright © 2011 John Wiley & Sons, Ltd.</abstract><doi>10.1002/ptr.3671</doi><tpages>7</tpages></addata></record> |
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| ispartof | Phytotherapy research, 2012-07, Vol.26 (7), p.988-994 |
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| source | Wiley Online Library Journals Frontfile Complete |
| title | Ampelopsin Inhibits H 2 O 2 ‐induced Apoptosis by ERK and Akt Signaling Pathways and Up‐regulation of Heme Oxygenase‐1 |
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