Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L
BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2010-11, Vol.66 (11), p.1257-1262 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1262 |
|---|---|
| container_issue | 11 |
| container_start_page | 1257 |
| container_title | Pest management science |
| container_volume | 66 |
| creator | Markussen, Mette DK Kristensen, Michael |
| description | BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only partial and indicated possible target-site resistance. The nAChR α2 subunit is important in neonicotinoid toxicity to insects, and gene expression of the Mdα2 subunit was investigated in field populations and laboratory strains of neonicotinoid-resistant and insecticide-susceptible houseflies, Musca domestica L. The genomic sequence covering exon III-VII of Mdα2 was analysed for mutations.RESULTS: Gene expression profiling of Mdα2 revealed notable differences between neonicotinoid-resistant and insecticide-susceptible houseflies. On average, the neonicotinoid-resistant field population 766b and the imidacloprid selected strain 791imi had 60% lower copy numbers of Mdα2 compared with the susceptible reference strain. Sequencing of exon III-VII of the Mdα2, encoding acetylcholine binding-site regions and three out of four transmembrane domains, did not reveal any mutations explaining the increased neonicotinoid tolerance in the strains examined.CONCLUSION: Previous discoveries and the results of this study suggest that the neonicotinoid resistance mechanism in Danish houseflies involves both cytochrome P450 monooxygenase-mediated detoxification and reduced expression of the nAChR subunit α2. Copyright |
| doi_str_mv | 10.1002/ps.2007 |
| format | Article |
| fullrecord | <record><control><sourceid>istex_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_ps_2007</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_WNG_KG5NKNB0_T</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3237-35a87107eeac55dbc4913548ddace5ed0ed403ce23fc4ee3a599cb13d548cc543</originalsourceid><addsrcrecordid>eNp10MFuEzEQBmALgdrSVrwB-FJxQNuO1-vs-thWEFDTFIlWcLMcexYMG3tlb9TksXiRPhOONoQTF3sOn2fGPyGvGJwzgPKiT-clQP2MHDFRTopKyub5vm6-HZKXKf0EAClleUAOS6g51A0_IutZeKS47iOm5IKnoaXemTC4fFJtcNh05kfonEca0WA_hEjTarHybqC39ul3SZ2nHsPuUXC2yK1cGrQfaBqidj5tm96uktHUhiWmweVqdkJetLpLeLq7j8nDh_f31x-L2d300_XlrDC85HXBhW5qBjWiNkLYhakk46JqrM3LCbSAtgJusOStqRC5FlKaBeM2G2NExY_J27GviSGliK3qo1vquFEM1DY71Se1zS7L16PsV4sl2r37G1YGZzug82e6NmpvXPrnOAc54Vv3bnSPrsPN_-apz192Y4tR59Bwvdc6_lKTmtdCfZ1P1c1UzG_mV6Dus38z-lYHpb_HvMFD7sQ4MAlQyYr_AdURoDU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Markussen, Mette DK ; Kristensen, Michael</creator><creatorcontrib>Markussen, Mette DK ; Kristensen, Michael</creatorcontrib><description>BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only partial and indicated possible target-site resistance. The nAChR α2 subunit is important in neonicotinoid toxicity to insects, and gene expression of the Mdα2 subunit was investigated in field populations and laboratory strains of neonicotinoid-resistant and insecticide-susceptible houseflies, Musca domestica L. The genomic sequence covering exon III-VII of Mdα2 was analysed for mutations.RESULTS: Gene expression profiling of Mdα2 revealed notable differences between neonicotinoid-resistant and insecticide-susceptible houseflies. On average, the neonicotinoid-resistant field population 766b and the imidacloprid selected strain 791imi had 60% lower copy numbers of Mdα2 compared with the susceptible reference strain. Sequencing of exon III-VII of the Mdα2, encoding acetylcholine binding-site regions and three out of four transmembrane domains, did not reveal any mutations explaining the increased neonicotinoid tolerance in the strains examined.CONCLUSION: Previous discoveries and the results of this study suggest that the neonicotinoid resistance mechanism in Danish houseflies involves both cytochrome P450 monooxygenase-mediated detoxification and reduced expression of the nAChR subunit α2. Copyright</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.2007</identifier><identifier>PMID: 20730783</identifier><identifier>CODEN: PMSCFC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; bioassays ; Biological and medical sciences ; cholinergic receptors ; cytochrome P-450 ; DNA Mutational Analysis ; exons ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; genes ; Houseflies - drug effects ; Houseflies - genetics ; Houseflies - metabolism ; imidacloprid ; Imidazoles ; insect pests ; Insect Proteins - chemistry ; Insect Proteins - genetics ; insecticide resistance ; Insecticide Resistance - genetics ; Insecticides ; Male ; metabolic detoxification ; molecular sequence data ; Musca domestica ; mutation ; nAChR subunit Mdα2 ; neonicotinoid insecticides ; Neonicotinoids ; Nitro Compounds ; pesticide synergists ; protein subunits ; Protein Subunits - genetics ; protein synthesis ; receptors ; Receptors, Nicotinic - genetics ; resistance mechanism ; sequence analysis</subject><ispartof>Pest management science, 2010-11, Vol.66 (11), p.1257-1262</ispartof><rights>Copyright © 2010 Society of Chemical Industry</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Society of Chemical Industry.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3237-35a87107eeac55dbc4913548ddace5ed0ed403ce23fc4ee3a599cb13d548cc543</citedby><cites>FETCH-LOGICAL-c3237-35a87107eeac55dbc4913548ddace5ed0ed403ce23fc4ee3a599cb13d548cc543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.2007$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.2007$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23309633$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20730783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Markussen, Mette DK</creatorcontrib><creatorcontrib>Kristensen, Michael</creatorcontrib><title>Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L</title><title>Pest management science</title><addtitle>Pest. Manag. Sci</addtitle><description>BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only partial and indicated possible target-site resistance. The nAChR α2 subunit is important in neonicotinoid toxicity to insects, and gene expression of the Mdα2 subunit was investigated in field populations and laboratory strains of neonicotinoid-resistant and insecticide-susceptible houseflies, Musca domestica L. The genomic sequence covering exon III-VII of Mdα2 was analysed for mutations.RESULTS: Gene expression profiling of Mdα2 revealed notable differences between neonicotinoid-resistant and insecticide-susceptible houseflies. On average, the neonicotinoid-resistant field population 766b and the imidacloprid selected strain 791imi had 60% lower copy numbers of Mdα2 compared with the susceptible reference strain. Sequencing of exon III-VII of the Mdα2, encoding acetylcholine binding-site regions and three out of four transmembrane domains, did not reveal any mutations explaining the increased neonicotinoid tolerance in the strains examined.CONCLUSION: Previous discoveries and the results of this study suggest that the neonicotinoid resistance mechanism in Danish houseflies involves both cytochrome P450 monooxygenase-mediated detoxification and reduced expression of the nAChR subunit α2. Copyright</description><subject>Animals</subject><subject>bioassays</subject><subject>Biological and medical sciences</subject><subject>cholinergic receptors</subject><subject>cytochrome P-450</subject><subject>DNA Mutational Analysis</subject><subject>exons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Houseflies - drug effects</subject><subject>Houseflies - genetics</subject><subject>Houseflies - metabolism</subject><subject>imidacloprid</subject><subject>Imidazoles</subject><subject>insect pests</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides</subject><subject>Male</subject><subject>metabolic detoxification</subject><subject>molecular sequence data</subject><subject>Musca domestica</subject><subject>mutation</subject><subject>nAChR subunit Mdα2</subject><subject>neonicotinoid insecticides</subject><subject>Neonicotinoids</subject><subject>Nitro Compounds</subject><subject>pesticide synergists</subject><subject>protein subunits</subject><subject>Protein Subunits - genetics</subject><subject>protein synthesis</subject><subject>receptors</subject><subject>Receptors, Nicotinic - genetics</subject><subject>resistance mechanism</subject><subject>sequence analysis</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFuEzEQBmALgdrSVrwB-FJxQNuO1-vs-thWEFDTFIlWcLMcexYMG3tlb9TksXiRPhOONoQTF3sOn2fGPyGvGJwzgPKiT-clQP2MHDFRTopKyub5vm6-HZKXKf0EAClleUAOS6g51A0_IutZeKS47iOm5IKnoaXemTC4fFJtcNh05kfonEca0WA_hEjTarHybqC39ul3SZ2nHsPuUXC2yK1cGrQfaBqidj5tm96uktHUhiWmweVqdkJetLpLeLq7j8nDh_f31x-L2d300_XlrDC85HXBhW5qBjWiNkLYhakk46JqrM3LCbSAtgJusOStqRC5FlKaBeM2G2NExY_J27GviSGliK3qo1vquFEM1DY71Se1zS7L16PsV4sl2r37G1YGZzug82e6NmpvXPrnOAc54Vv3bnSPrsPN_-apz192Y4tR59Bwvdc6_lKTmtdCfZ1P1c1UzG_mV6Dus38z-lYHpb_HvMFD7sQ4MAlQyYr_AdURoDU</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Markussen, Mette DK</creator><creator>Kristensen, Michael</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201011</creationdate><title>Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L</title><author>Markussen, Mette DK ; Kristensen, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3237-35a87107eeac55dbc4913548ddace5ed0ed403ce23fc4ee3a599cb13d548cc543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>bioassays</topic><topic>Biological and medical sciences</topic><topic>cholinergic receptors</topic><topic>cytochrome P-450</topic><topic>DNA Mutational Analysis</topic><topic>exons</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Houseflies - drug effects</topic><topic>Houseflies - genetics</topic><topic>Houseflies - metabolism</topic><topic>imidacloprid</topic><topic>Imidazoles</topic><topic>insect pests</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>Insecticides</topic><topic>Male</topic><topic>metabolic detoxification</topic><topic>molecular sequence data</topic><topic>Musca domestica</topic><topic>mutation</topic><topic>nAChR subunit Mdα2</topic><topic>neonicotinoid insecticides</topic><topic>Neonicotinoids</topic><topic>Nitro Compounds</topic><topic>pesticide synergists</topic><topic>protein subunits</topic><topic>Protein Subunits - genetics</topic><topic>protein synthesis</topic><topic>receptors</topic><topic>Receptors, Nicotinic - genetics</topic><topic>resistance mechanism</topic><topic>sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markussen, Mette DK</creatorcontrib><creatorcontrib>Kristensen, Michael</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markussen, Mette DK</au><au>Kristensen, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L</atitle><jtitle>Pest management science</jtitle><addtitle>Pest. Manag. Sci</addtitle><date>2010-11</date><risdate>2010</risdate><volume>66</volume><issue>11</issue><spage>1257</spage><epage>1262</epage><pages>1257-1262</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><coden>PMSCFC</coden><abstract>BACKGROUND: Neonicotinoid action as well as resistance involves interaction with nicotinic acetylcholine receptors (nAChRs). In the housefly, neonicotinoid resistance also involves cytochrome P450, as indicated by bioassay with synergist as well as altered expression. In bioassay, synergism was only partial and indicated possible target-site resistance. The nAChR α2 subunit is important in neonicotinoid toxicity to insects, and gene expression of the Mdα2 subunit was investigated in field populations and laboratory strains of neonicotinoid-resistant and insecticide-susceptible houseflies, Musca domestica L. The genomic sequence covering exon III-VII of Mdα2 was analysed for mutations.RESULTS: Gene expression profiling of Mdα2 revealed notable differences between neonicotinoid-resistant and insecticide-susceptible houseflies. On average, the neonicotinoid-resistant field population 766b and the imidacloprid selected strain 791imi had 60% lower copy numbers of Mdα2 compared with the susceptible reference strain. Sequencing of exon III-VII of the Mdα2, encoding acetylcholine binding-site regions and three out of four transmembrane domains, did not reveal any mutations explaining the increased neonicotinoid tolerance in the strains examined.CONCLUSION: Previous discoveries and the results of this study suggest that the neonicotinoid resistance mechanism in Danish houseflies involves both cytochrome P450 monooxygenase-mediated detoxification and reduced expression of the nAChR subunit α2. Copyright</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>20730783</pmid><doi>10.1002/ps.2007</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2010-11, Vol.66 (11), p.1257-1262 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_ps_2007 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals bioassays Biological and medical sciences cholinergic receptors cytochrome P-450 DNA Mutational Analysis exons Female Fundamental and applied biological sciences. Psychology Gene Expression genes Houseflies - drug effects Houseflies - genetics Houseflies - metabolism imidacloprid Imidazoles insect pests Insect Proteins - chemistry Insect Proteins - genetics insecticide resistance Insecticide Resistance - genetics Insecticides Male metabolic detoxification molecular sequence data Musca domestica mutation nAChR subunit Mdα2 neonicotinoid insecticides Neonicotinoids Nitro Compounds pesticide synergists protein subunits Protein Subunits - genetics protein synthesis receptors Receptors, Nicotinic - genetics resistance mechanism sequence analysis |
| title | Low expression of nicotinic acetylcholine receptor subunit Mdα2 in neonicotinoid-resistant strains of Musca domestica L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T11%3A07%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-istex_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low%20expression%20of%20nicotinic%20acetylcholine%20receptor%20subunit%20Md%CE%B12%20in%20neonicotinoid-resistant%20strains%20of%20Musca%20domestica%20L&rft.jtitle=Pest%20management%20science&rft.au=Markussen,%20Mette%20DK&rft.date=2010-11&rft.volume=66&rft.issue=11&rft.spage=1257&rft.epage=1262&rft.pages=1257-1262&rft.issn=1526-498X&rft.eissn=1526-4998&rft.coden=PMSCFC&rft_id=info:doi/10.1002/ps.2007&rft_dat=%3Cistex_cross%3Eark_67375_WNG_KG5NKNB0_T%3C/istex_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/20730783&rfr_iscdi=true |