Analysis of 13 C and 14 C labeling in pyruvate and lactate in tumor and blood of lymphoma-bearing mice injected with 13 C- and 14 C-labeled pyruvate

Measurements of hyperpolarized C label exchange between injected [1- C]pyruvate and the endogenous tumor lactate pool can give an apparent first-order rate constant for the exchange. The determination of the isotope flux, however, requires an estimate of the labeled pyruvate concentration in the tum...

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Veröffentlicht in:NMR in biomedicine 2018-05, Vol.31 (5), p.e3901
Hauptverfasser: Serrao, E M, Kettunen, M I, Rodrigues, T B, Lewis, D Y, Gallagher, F A, Hu, D E, Brindle, K M
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container_issue 5
container_start_page e3901
container_title NMR in biomedicine
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creator Serrao, E M
Kettunen, M I
Rodrigues, T B
Lewis, D Y
Gallagher, F A
Hu, D E
Brindle, K M
description Measurements of hyperpolarized C label exchange between injected [1- C]pyruvate and the endogenous tumor lactate pool can give an apparent first-order rate constant for the exchange. The determination of the isotope flux, however, requires an estimate of the labeled pyruvate concentration in the tumor. This was achieved here by measurement of the tumor uptake of [1- C]pyruvate, which showed that
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The determination of the isotope flux, however, requires an estimate of the labeled pyruvate concentration in the tumor. This was achieved here by measurement of the tumor uptake of [1- C]pyruvate, which showed that &lt;2% of the injected pyruvate reached the tumor site. Multiplication of this estimated labeled pyruvate concentration in the tumor with the apparent first-order rate constant for hyperpolarized C label exchange gave an isotope flux that showed good agreement with a flux determined directly by the injection of non-polarized [3- C]pyruvate, rapid excision of the tumor after 30 s and measurement of C-labeled lactate concentrations in tumor extracts. The distribution of labeled lactate between intra- and extracellular compartments and the blood pool was investigated by imaging, by measurement of the labeled lactate concentration in blood and tumor, and by examination of the effects of a gadolinium contrast agent and a lactate transport inhibitor on the intensity of the hyperpolarized [1- C]lactate signal. These measurements showed that there was significant export of labeled lactate from the tumor, but that labeled lactate in the blood pool produced by the injection of hyperpolarized [1- C]pyruvate showed only relatively low levels of polarization. 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subjects Animals
Carbon Isotopes - metabolism
Carbon Radioisotopes - metabolism
Injections
Isotope Labeling
Lactic Acid - blood
Lymphoma - blood
Mice, Inbred C57BL
Pyruvic Acid - blood
Tissue Distribution
title Analysis of 13 C and 14 C labeling in pyruvate and lactate in tumor and blood of lymphoma-bearing mice injected with 13 C- and 14 C-labeled pyruvate
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