Involvement of superoxide radicals in the mouse two-cell block
The effect of oxygen toxicity on the development of mammalian embryos was asssessed by the use of superoxide dismutase (SOD), a potent scavenger of superoxide radicals. Mouse pronuclear embryos recovered 17 h after human chorionic gonadotropin (hCG) were cultured in medium BWW at 37°C under an atmos...
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| Veröffentlicht in: | Molecular reproduction and development 1991-04, Vol.28 (4), p.356-360 |
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| container_title | Molecular reproduction and development |
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| creator | Noda, Yoichi Matsumoto, Hisashi Umaoka, Yoh Tatsumi, Kenichi Kishi, Junji Mori, Takahide |
| description | The effect of oxygen toxicity on the development of mammalian embryos was asssessed by the use of superoxide dismutase (SOD), a potent scavenger of superoxide radicals. Mouse pronuclear embryos recovered 17 h after human chorionic gonadotropin (hCG) were cultured in medium BWW at 37°C under an atmosphere of 5% CO2 in air. Culture of mouse pronuclear embryos in the presence of Cu ∣ Zn‐SOD (500 μg/ml) significantly increased the blastulation rate (44.6%) when compared with the control culture system (4.2%). Essentially the same effects were observed in SOD containing either Mn or Fe in the catalytic center. Heat treatment of the SOD preparation, and the addition of anti‐SOD antibodies to the culture medium, significantly reduced the attenuation of the two‐cell block by SOD, indicating that this effect is SOD dependent. SOD activity was detected in rabbit oviduct fluid (3,675 ± 3,084 mlU/mg protein) by electron spin resonance. These results suggest that active oxygen is involved in the two‐cell block phenomenon in mouse embryos exposed to air and that SOD in the oviduct may play an important role in the protection of embryos from superoxide radicals. |
| doi_str_mv | 10.1002/mrd.1080280408 |
| format | Article |
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Mouse pronuclear embryos recovered 17 h after human chorionic gonadotropin (hCG) were cultured in medium BWW at 37°C under an atmosphere of 5% CO2 in air. Culture of mouse pronuclear embryos in the presence of Cu ∣ Zn‐SOD (500 μg/ml) significantly increased the blastulation rate (44.6%) when compared with the control culture system (4.2%). Essentially the same effects were observed in SOD containing either Mn or Fe in the catalytic center. Heat treatment of the SOD preparation, and the addition of anti‐SOD antibodies to the culture medium, significantly reduced the attenuation of the two‐cell block by SOD, indicating that this effect is SOD dependent. SOD activity was detected in rabbit oviduct fluid (3,675 ± 3,084 mlU/mg protein) by electron spin resonance. These results suggest that active oxygen is involved in the two‐cell block phenomenon in mouse embryos exposed to air and that SOD in the oviduct may play an important role in the protection of embryos from superoxide radicals.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.1080280408</identifier><identifier>PMID: 1648368</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Culture Techniques ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryo development ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development ; Female ; Fundamental and applied biological sciences. Psychology ; Mice ; Oxygen - toxicity ; Rabbits ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Superoxides - metabolism</subject><ispartof>Molecular reproduction and development, 1991-04, Vol.28 (4), p.356-360</ispartof><rights>Copyright © 1991 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3898-56d9c46eea3ec5e6b7f770b1d7c5e97f6f961402328ad4e9904dfc027e1275bd3</citedby><cites>FETCH-LOGICAL-c3898-56d9c46eea3ec5e6b7f770b1d7c5e97f6f961402328ad4e9904dfc027e1275bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.1080280408$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.1080280408$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19623738$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1648368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noda, Yoichi</creatorcontrib><creatorcontrib>Matsumoto, Hisashi</creatorcontrib><creatorcontrib>Umaoka, Yoh</creatorcontrib><creatorcontrib>Tatsumi, Kenichi</creatorcontrib><creatorcontrib>Kishi, Junji</creatorcontrib><creatorcontrib>Mori, Takahide</creatorcontrib><title>Involvement of superoxide radicals in the mouse two-cell block</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>The effect of oxygen toxicity on the development of mammalian embryos was asssessed by the use of superoxide dismutase (SOD), a potent scavenger of superoxide radicals. Mouse pronuclear embryos recovered 17 h after human chorionic gonadotropin (hCG) were cultured in medium BWW at 37°C under an atmosphere of 5% CO2 in air. Culture of mouse pronuclear embryos in the presence of Cu ∣ Zn‐SOD (500 μg/ml) significantly increased the blastulation rate (44.6%) when compared with the control culture system (4.2%). Essentially the same effects were observed in SOD containing either Mn or Fe in the catalytic center. Heat treatment of the SOD preparation, and the addition of anti‐SOD antibodies to the culture medium, significantly reduced the attenuation of the two‐cell block by SOD, indicating that this effect is SOD dependent. SOD activity was detected in rabbit oviduct fluid (3,675 ± 3,084 mlU/mg protein) by electron spin resonance. These results suggest that active oxygen is involved in the two‐cell block phenomenon in mouse embryos exposed to air and that SOD in the oviduct may play an important role in the protection of embryos from superoxide radicals.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Culture Techniques</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryo development</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Oxygen - toxicity</subject><subject>Rabbits</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxides - metabolism</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLw0AQxhdRfF-9Cbl4jM4-so-LoFWr4gOKorcl2Z1gNGnKbmvrf29KxOLJ08wwv28eHyEHFI4pADtpgu8SDUyDAL1GtikYnTJlsvVlLiAVGXvdIjsxvgOAMRo2ySaVQnOpt8npzfizrT-xwfE0acskziYY2kXlMQm5r1xex6QaJ9M3TJp2FjGZztvUYV0nRd26jz2yUXYI7v_EXfJ8dfk0uE7vHoc3g7O71HHdnZNJb5yQiDlHl6EsVKkUFNSrrjKqlKWRVADjTOdeoDEgfOmAKaRMZYXnu-S4n-tCG2PA0k5C1eThy1KwSx9s54Nd-dAJDnvBZFY06Fd4_3jXP_rp57F7sgz52FVxhRnJuOJLzvTcvKrx65-t9n508eeGtNdWcYqLX20ePqxUXGX25WFo6Wg0un05H1rBvwGwdIWU</recordid><startdate>199104</startdate><enddate>199104</enddate><creator>Noda, Yoichi</creator><creator>Matsumoto, Hisashi</creator><creator>Umaoka, Yoh</creator><creator>Tatsumi, Kenichi</creator><creator>Kishi, Junji</creator><creator>Mori, Takahide</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199104</creationdate><title>Involvement of superoxide radicals in the mouse two-cell block</title><author>Noda, Yoichi ; Matsumoto, Hisashi ; Umaoka, Yoh ; Tatsumi, Kenichi ; Kishi, Junji ; Mori, Takahide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3898-56d9c46eea3ec5e6b7f770b1d7c5e97f6f961402328ad4e9904dfc027e1275bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Culture Techniques</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryo development</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Oxygen - toxicity</topic><topic>Rabbits</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noda, Yoichi</creatorcontrib><creatorcontrib>Matsumoto, Hisashi</creatorcontrib><creatorcontrib>Umaoka, Yoh</creatorcontrib><creatorcontrib>Tatsumi, Kenichi</creatorcontrib><creatorcontrib>Kishi, Junji</creatorcontrib><creatorcontrib>Mori, Takahide</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noda, Yoichi</au><au>Matsumoto, Hisashi</au><au>Umaoka, Yoh</au><au>Tatsumi, Kenichi</au><au>Kishi, Junji</au><au>Mori, Takahide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of superoxide radicals in the mouse two-cell block</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1991-04</date><risdate>1991</risdate><volume>28</volume><issue>4</issue><spage>356</spage><epage>360</epage><pages>356-360</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>The effect of oxygen toxicity on the development of mammalian embryos was asssessed by the use of superoxide dismutase (SOD), a potent scavenger of superoxide radicals. Mouse pronuclear embryos recovered 17 h after human chorionic gonadotropin (hCG) were cultured in medium BWW at 37°C under an atmosphere of 5% CO2 in air. Culture of mouse pronuclear embryos in the presence of Cu ∣ Zn‐SOD (500 μg/ml) significantly increased the blastulation rate (44.6%) when compared with the control culture system (4.2%). Essentially the same effects were observed in SOD containing either Mn or Fe in the catalytic center. Heat treatment of the SOD preparation, and the addition of anti‐SOD antibodies to the culture medium, significantly reduced the attenuation of the two‐cell block by SOD, indicating that this effect is SOD dependent. SOD activity was detected in rabbit oviduct fluid (3,675 ± 3,084 mlU/mg protein) by electron spin resonance. These results suggest that active oxygen is involved in the two‐cell block phenomenon in mouse embryos exposed to air and that SOD in the oviduct may play an important role in the protection of embryos from superoxide radicals.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1648368</pmid><doi>10.1002/mrd.1080280408</doi><tpages>5</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1991-04, Vol.28 (4), p.356-360 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Culture Techniques Early stages. Segmentation. Gastrulation. Neurulation Embryo development Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development Female Fundamental and applied biological sciences. Psychology Mice Oxygen - toxicity Rabbits Superoxide dismutase Superoxide Dismutase - metabolism Superoxides - metabolism |
| title | Involvement of superoxide radicals in the mouse two-cell block |
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