Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression
A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the pres...
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| Veröffentlicht in: | Macromolecular bioscience 2012-05, Vol.12 (5), p.675-691 |
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| creator | Lammers, Gerwen Roth, Günter Heck, Mathias Zengerle, Roland Tjabringa, G. Sandra Versteeg, Elly M. Hafmans, Theo Wismans, Ronnie Reinhardt, Dieter P. Verwiel, Eugene T. P. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost Brock, Roland Daamen, Willeke F. van Kuppevelt, Toin H. |
| description | A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT‐qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.
Tissue‐engineered skin often lacks the architecture of the papillary dermis that is present in normal skin. Therefore, the construction of a fibrillar collagen membrane mimicking the 3D‐architecture of the human papillary dermis is described. Evaluation with cultured keratinocytes shows that cell morphology, gene and protein expression are directed by this membrane structure. |
| doi_str_mv | 10.1002/mabi.201100443 |
| format | Article |
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Tissue‐engineered skin often lacks the architecture of the papillary dermis that is present in normal skin. Therefore, the construction of a fibrillar collagen membrane mimicking the 3D‐architecture of the human papillary dermis is described. Evaluation with cultured keratinocytes shows that cell morphology, gene and protein expression are directed by this membrane structure.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201100443</identifier><identifier>PMID: 22416023</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Applied sciences ; Artificial membranes and reconstituted systems ; Biological and medical sciences ; biomedical applications ; Biomimetic Materials - chemistry ; Biomimetic Materials - pharmacology ; biomimetics ; Cell Differentiation - drug effects ; Collagen - chemistry ; Collagen - pharmacology ; Collagen - ultrastructure ; Dermis - cytology ; Dermis - drug effects ; Dermis - metabolism ; Exact sciences and technology ; Exchange resins and membranes ; Extracellular Matrix - drug effects ; Extracellular Matrix - metabolism ; Forms of application and semi-finished materials ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Gene Expression Profiling ; Humans ; Immunohistochemistry ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Medical sciences ; Membrane physicochemistry ; Membranes, Artificial ; Microscopy, Electron, Scanning ; microstructures ; Molecular biophysics ; Oligonucleotide Array Sequence Analysis ; Polymer industry, paints, wood ; Primary Cell Culture ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology of polymers ; Technology. Biomaterials. Equipments ; tissue engineering ; Tissue Scaffolds ; Wound Healing - physiology</subject><ispartof>Macromolecular bioscience, 2012-05, Vol.12 (5), p.675-691</ispartof><rights>Copyright © 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4793-7f894bb5ce70e82f7a977d43d90152b5a78918dfe82f5539405a1bfe031e6b93</citedby><cites>FETCH-LOGICAL-c4793-7f894bb5ce70e82f7a977d43d90152b5a78918dfe82f5539405a1bfe031e6b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201100443$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201100443$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25867661$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22416023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lammers, Gerwen</creatorcontrib><creatorcontrib>Roth, Günter</creatorcontrib><creatorcontrib>Heck, Mathias</creatorcontrib><creatorcontrib>Zengerle, Roland</creatorcontrib><creatorcontrib>Tjabringa, G. Sandra</creatorcontrib><creatorcontrib>Versteeg, Elly M.</creatorcontrib><creatorcontrib>Hafmans, Theo</creatorcontrib><creatorcontrib>Wismans, Ronnie</creatorcontrib><creatorcontrib>Reinhardt, Dieter P.</creatorcontrib><creatorcontrib>Verwiel, Eugene T. P.</creatorcontrib><creatorcontrib>Zeeuwen, Patrick L. J. M.</creatorcontrib><creatorcontrib>Schalkwijk, Joost</creatorcontrib><creatorcontrib>Brock, Roland</creatorcontrib><creatorcontrib>Daamen, Willeke F.</creatorcontrib><creatorcontrib>van Kuppevelt, Toin H.</creatorcontrib><title>Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT‐qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.
Tissue‐engineered skin often lacks the architecture of the papillary dermis that is present in normal skin. Therefore, the construction of a fibrillar collagen membrane mimicking the 3D‐architecture of the human papillary dermis is described. Evaluation with cultured keratinocytes shows that cell morphology, gene and protein expression are directed by this membrane structure.</description><subject>Applied sciences</subject><subject>Artificial membranes and reconstituted systems</subject><subject>Biological and medical sciences</subject><subject>biomedical applications</subject><subject>Biomimetic Materials - chemistry</subject><subject>Biomimetic Materials - pharmacology</subject><subject>biomimetics</subject><subject>Cell Differentiation - drug effects</subject><subject>Collagen - chemistry</subject><subject>Collagen - pharmacology</subject><subject>Collagen - ultrastructure</subject><subject>Dermis - cytology</subject><subject>Dermis - drug effects</subject><subject>Dermis - metabolism</subject><subject>Exact sciences and technology</subject><subject>Exchange resins and membranes</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - metabolism</subject><subject>Forms of application and semi-finished materials</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Medical sciences</subject><subject>Membrane physicochemistry</subject><subject>Membranes, Artificial</subject><subject>Microscopy, Electron, Scanning</subject><subject>microstructures</subject><subject>Molecular biophysics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymer industry, paints, wood</subject><subject>Primary Cell Culture</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology of polymers</subject><subject>Technology. Biomaterials. Equipments</subject><subject>tissue engineering</subject><subject>Tissue Scaffolds</subject><subject>Wound Healing - physiology</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUhS1ERV9sWSJvWGaw4ziOlzNDOxQ6pZUqdWk5zs2MaRJHdkZ0fkn_Lg4pU3as_PrO8T0HoQ-UzCgh6edWl3aWEhoPWcbeoBOa0zzhVPK3h30hjtFpCD8JoaKQ6Tt0nKYZzUnKTtDz0nVh8DszWNdhV2ON19Z4N93tPFR46ZpGb6DDa2hLrzuIRGvNo-02eNgCvtW9jYTf4y_gWxvw3JutHeCPHOuuwqudrUb6O3g92M6Z_RBNnO-3rnGb_cRANL546j2EEEc5R0e1bgK8f1nP0P3lxf3ya3L9Y3W1nF8nJhOSJaIuZFaW3IAgUKS10FKIKmOVJJSnJdcxMC2qenzjnMmMcE3LGgijkJeSnaHZZDtGDh5q1XvbxiyKEjUWrMaC1aHgKPg4Cfpd2UJ1wP82GoFPL4AORjd1LMzY8MrxIhd5TiMnJ-6XbWD_n2_Ver64-neIZNLaMMDTQav9o8oFE1w93KzU4oZeflvcCcXYbxw6prE</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Lammers, Gerwen</creator><creator>Roth, Günter</creator><creator>Heck, Mathias</creator><creator>Zengerle, Roland</creator><creator>Tjabringa, G. Sandra</creator><creator>Versteeg, Elly M.</creator><creator>Hafmans, Theo</creator><creator>Wismans, Ronnie</creator><creator>Reinhardt, Dieter P.</creator><creator>Verwiel, Eugene T. P.</creator><creator>Zeeuwen, Patrick L. J. M.</creator><creator>Schalkwijk, Joost</creator><creator>Brock, Roland</creator><creator>Daamen, Willeke F.</creator><creator>van Kuppevelt, Toin H.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201205</creationdate><title>Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression</title><author>Lammers, Gerwen ; Roth, Günter ; Heck, Mathias ; Zengerle, Roland ; Tjabringa, G. Sandra ; Versteeg, Elly M. ; Hafmans, Theo ; Wismans, Ronnie ; Reinhardt, Dieter P. ; Verwiel, Eugene T. P. ; Zeeuwen, Patrick L. J. 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Psychology</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Medical sciences</topic><topic>Membrane physicochemistry</topic><topic>Membranes, Artificial</topic><topic>Microscopy, Electron, Scanning</topic><topic>microstructures</topic><topic>Molecular biophysics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymer industry, paints, wood</topic><topic>Primary Cell Culture</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology of polymers</topic><topic>Technology. Biomaterials. 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M.</creatorcontrib><creatorcontrib>Schalkwijk, Joost</creatorcontrib><creatorcontrib>Brock, Roland</creatorcontrib><creatorcontrib>Daamen, Willeke F.</creatorcontrib><creatorcontrib>van Kuppevelt, Toin H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lammers, Gerwen</au><au>Roth, Günter</au><au>Heck, Mathias</au><au>Zengerle, Roland</au><au>Tjabringa, G. Sandra</au><au>Versteeg, Elly M.</au><au>Hafmans, Theo</au><au>Wismans, Ronnie</au><au>Reinhardt, Dieter P.</au><au>Verwiel, Eugene T. P.</au><au>Zeeuwen, Patrick L. J. M.</au><au>Schalkwijk, Joost</au><au>Brock, Roland</au><au>Daamen, Willeke F.</au><au>van Kuppevelt, Toin H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2012-05</date><risdate>2012</risdate><volume>12</volume><issue>5</issue><spage>675</spage><epage>691</epage><pages>675-691</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT‐qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.
Tissue‐engineered skin often lacks the architecture of the papillary dermis that is present in normal skin. Therefore, the construction of a fibrillar collagen membrane mimicking the 3D‐architecture of the human papillary dermis is described. Evaluation with cultured keratinocytes shows that cell morphology, gene and protein expression are directed by this membrane structure.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>22416023</pmid><doi>10.1002/mabi.201100443</doi><tpages>17</tpages></addata></record> |
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| ispartof | Macromolecular bioscience, 2012-05, Vol.12 (5), p.675-691 |
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| subjects | Applied sciences Artificial membranes and reconstituted systems Biological and medical sciences biomedical applications Biomimetic Materials - chemistry Biomimetic Materials - pharmacology biomimetics Cell Differentiation - drug effects Collagen - chemistry Collagen - pharmacology Collagen - ultrastructure Dermis - cytology Dermis - drug effects Dermis - metabolism Exact sciences and technology Exchange resins and membranes Extracellular Matrix - drug effects Extracellular Matrix - metabolism Forms of application and semi-finished materials Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Gene Expression Profiling Humans Immunohistochemistry Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Medical sciences Membrane physicochemistry Membranes, Artificial Microscopy, Electron, Scanning microstructures Molecular biophysics Oligonucleotide Array Sequence Analysis Polymer industry, paints, wood Primary Cell Culture Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology of polymers Technology. Biomaterials. Equipments tissue engineering Tissue Scaffolds Wound Healing - physiology |
| title | Construction of a Microstructured Collagen Membrane Mimicking the Papillary Dermis Architecture and Guiding Keratinocyte Morphology and Gene Expression |
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