Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects
Background and Objective Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld break...
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| Veröffentlicht in: | Lasers in surgery and medicine 2001-01, Vol.28 (4), p.297-306 |
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| creator | Sorg, Brian S. Welch, Ashley J. |
| description | Background and Objective
Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld breaking strength is studied. Additionally, the effect of hydration on the strength of the reinforced welds is investigated.
Study Design/Materials and Methods
A 50%(w/v) bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806‐nm CW diode laser. A poly(DL‐lactic‐co‐glycolic acid) (PLGA) film was used to reinforce the solder (the controls had solder but no reinforcement). Breaking strengths were measured acutely and after hydration in saline for 1 and 2 days. The data were analyzed by ANOVA (P |
| doi_str_mv | 10.1002/lsm.1053 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_lsm_1053</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>LSM1053</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3843-ad51e60c94b1a287a9a693fd5da8f707fcf78343cebe019471156668dcb3bdc3</originalsourceid><addsrcrecordid>eNp10MFu1DAQBmALUdGlIPEEyAcOvYTacRIn3FDVLUhpK5VKcLMm9njXrROv7Cwlb0-qjUovnGZG-jSj-Qn5wNlnzlh-5lM_N6V4RVacNVXWcMZfkxXjc1-zJj8mb1O6Z4yJnMk35JhzURQlq1cktJAwZqNLaY80BW8wumFDH924pZ0LBjcRDHQe6S74qcdIrfN9om6gv90Ywxe6nmea9tGCRtqHuNsGHzYThcHQ7WQijC4MFK1FPaZ35MiCT_h-qSfkbn1xd_4ta28uv59_bTMt6kJkYEqOFdNN0XHIawkNVI2wpjRQW8mk1VbWohAaO5y_LCTnZVVVtdGd6IwWJ-T0sFbHkFJEq3bR9RAnxZl6ikzNkamnyGb68UB3-65H8w8uGc3g0wIgafA2wqBderGwbGTezCw7sEfncfrvPdX-uFruLt6lEf88e4gPqpJClurn9aUqWbu-zX9xVYu_ebqTNg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Sorg, Brian S. ; Welch, Ashley J.</creator><creatorcontrib>Sorg, Brian S. ; Welch, Ashley J.</creatorcontrib><description>Background and Objective
Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld breaking strength is studied. Additionally, the effect of hydration on the strength of the reinforced welds is investigated.
Study Design/Materials and Methods
A 50%(w/v) bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806‐nm CW diode laser. A poly(DL‐lactic‐co‐glycolic acid) (PLGA) film was used to reinforce the solder (the controls had solder but no reinforcement). Breaking strengths were measured acutely and after hydration in saline for 1 and 2 days. The data were analyzed by ANOVA (P < 0.05) and multiple comparisons of means were performed using the Newman–Keuls test.
Results
The creation of pores in the PLGA films qualitatively improved the film flexibility without having an apparent adverse effect on the breaking strength, while the actual technique of applying the film and solder had more of an effect. The acute maximum average breaking strengths of some of the film reinforced specimens (114.7 g–134.4 g) were significantly higher (P < 0.05) than the acute maximum average breaking strength of the unreinforced control specimens (68.3 g). Film reinforced specimens were shown to have a statistically significantly higher breaking strength than unreinforced controls after 1‐ and 2‐day hydration.
Conclusions
Reinforcement of liquid albumin solders in laser‐assisted incision repair appears to have advantages over conventional methods that do not reinforce the cohesive strength of the solder in terms of acute breaking strength and after immersion in moist environments for short periods of time. Using a film with the solder applied to one surface only may be advantageous over other techniques. Lasers Surg. Med. 28:297–306, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.1053</identifier><identifier>PMID: 11344508</identifier><identifier>CODEN: LSMEDI</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>albumin ; Animals ; Aorta, Thoracic - surgery ; Biocompatible Materials ; Biodegradation, Environmental ; Biological and medical sciences ; Cattle ; diode laser ; Diseases of the cardiovascular system ; failure modes ; In Vitro Techniques ; Lactic Acid ; Laser Coagulation ; Medical sciences ; Microscopy, Electron, Scanning ; Miscellaneous ; poly-(lactic-co-glycolic acid) ; Polyglycolic Acid ; Polymers ; Prostheses and Implants ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Serum Albumin, Bovine ; Tensile Strength ; tissue welding</subject><ispartof>Lasers in surgery and medicine, 2001-01, Vol.28 (4), p.297-306</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3843-ad51e60c94b1a287a9a693fd5da8f707fcf78343cebe019471156668dcb3bdc3</citedby><cites>FETCH-LOGICAL-c3843-ad51e60c94b1a287a9a693fd5da8f707fcf78343cebe019471156668dcb3bdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.1053$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.1053$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1059729$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11344508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorg, Brian S.</creatorcontrib><creatorcontrib>Welch, Ashley J.</creatorcontrib><title>Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg. Med</addtitle><description>Background and Objective
Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld breaking strength is studied. Additionally, the effect of hydration on the strength of the reinforced welds is investigated.
Study Design/Materials and Methods
A 50%(w/v) bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806‐nm CW diode laser. A poly(DL‐lactic‐co‐glycolic acid) (PLGA) film was used to reinforce the solder (the controls had solder but no reinforcement). Breaking strengths were measured acutely and after hydration in saline for 1 and 2 days. The data were analyzed by ANOVA (P < 0.05) and multiple comparisons of means were performed using the Newman–Keuls test.
Results
The creation of pores in the PLGA films qualitatively improved the film flexibility without having an apparent adverse effect on the breaking strength, while the actual technique of applying the film and solder had more of an effect. The acute maximum average breaking strengths of some of the film reinforced specimens (114.7 g–134.4 g) were significantly higher (P < 0.05) than the acute maximum average breaking strength of the unreinforced control specimens (68.3 g). Film reinforced specimens were shown to have a statistically significantly higher breaking strength than unreinforced controls after 1‐ and 2‐day hydration.
Conclusions
Reinforcement of liquid albumin solders in laser‐assisted incision repair appears to have advantages over conventional methods that do not reinforce the cohesive strength of the solder in terms of acute breaking strength and after immersion in moist environments for short periods of time. Using a film with the solder applied to one surface only may be advantageous over other techniques. Lasers Surg. Med. 28:297–306, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>albumin</subject><subject>Animals</subject><subject>Aorta, Thoracic - surgery</subject><subject>Biocompatible Materials</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>diode laser</subject><subject>Diseases of the cardiovascular system</subject><subject>failure modes</subject><subject>In Vitro Techniques</subject><subject>Lactic Acid</subject><subject>Laser Coagulation</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Miscellaneous</subject><subject>poly-(lactic-co-glycolic acid)</subject><subject>Polyglycolic Acid</subject><subject>Polymers</subject><subject>Prostheses and Implants</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Serum Albumin, Bovine</subject><subject>Tensile Strength</subject><subject>tissue welding</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFu1DAQBmALUdGlIPEEyAcOvYTacRIn3FDVLUhpK5VKcLMm9njXrROv7Cwlb0-qjUovnGZG-jSj-Qn5wNlnzlh-5lM_N6V4RVacNVXWcMZfkxXjc1-zJj8mb1O6Z4yJnMk35JhzURQlq1cktJAwZqNLaY80BW8wumFDH924pZ0LBjcRDHQe6S74qcdIrfN9om6gv90Ywxe6nmea9tGCRtqHuNsGHzYThcHQ7WQijC4MFK1FPaZ35MiCT_h-qSfkbn1xd_4ta28uv59_bTMt6kJkYEqOFdNN0XHIawkNVI2wpjRQW8mk1VbWohAaO5y_LCTnZVVVtdGd6IwWJ-T0sFbHkFJEq3bR9RAnxZl6ikzNkamnyGb68UB3-65H8w8uGc3g0wIgafA2wqBderGwbGTezCw7sEfncfrvPdX-uFruLt6lEf88e4gPqpJClurn9aUqWbu-zX9xVYu_ebqTNg</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Sorg, Brian S.</creator><creator>Welch, Ashley J.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20010101</creationdate><title>Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects</title><author>Sorg, Brian S. ; Welch, Ashley J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3843-ad51e60c94b1a287a9a693fd5da8f707fcf78343cebe019471156668dcb3bdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>albumin</topic><topic>Animals</topic><topic>Aorta, Thoracic - surgery</topic><topic>Biocompatible Materials</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>diode laser</topic><topic>Diseases of the cardiovascular system</topic><topic>failure modes</topic><topic>In Vitro Techniques</topic><topic>Lactic Acid</topic><topic>Laser Coagulation</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Miscellaneous</topic><topic>poly-(lactic-co-glycolic acid)</topic><topic>Polyglycolic Acid</topic><topic>Polymers</topic><topic>Prostheses and Implants</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Serum Albumin, Bovine</topic><topic>Tensile Strength</topic><topic>tissue welding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorg, Brian S.</creatorcontrib><creatorcontrib>Welch, Ashley J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorg, Brian S.</au><au>Welch, Ashley J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg. Med</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>28</volume><issue>4</issue><spage>297</spage><epage>306</epage><pages>297-306</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><coden>LSMEDI</coden><abstract>Background and Objective
Previous research introduced the concept of using biodegradable polymer film reinforcement of a liquid albumin solder for improvement of the tensile strength of repaired incisions in vitro. In this study, the effect of creating small pores in the PLGA films on the weld breaking strength is studied. Additionally, the effect of hydration on the strength of the reinforced welds is investigated.
Study Design/Materials and Methods
A 50%(w/v) bovine serum albumin solder with 0.5 mg/mL Indocyanine Green dye was used to repair an incision in bovine aorta. The solder was coagulated with an 806‐nm CW diode laser. A poly(DL‐lactic‐co‐glycolic acid) (PLGA) film was used to reinforce the solder (the controls had solder but no reinforcement). Breaking strengths were measured acutely and after hydration in saline for 1 and 2 days. The data were analyzed by ANOVA (P < 0.05) and multiple comparisons of means were performed using the Newman–Keuls test.
Results
The creation of pores in the PLGA films qualitatively improved the film flexibility without having an apparent adverse effect on the breaking strength, while the actual technique of applying the film and solder had more of an effect. The acute maximum average breaking strengths of some of the film reinforced specimens (114.7 g–134.4 g) were significantly higher (P < 0.05) than the acute maximum average breaking strength of the unreinforced control specimens (68.3 g). Film reinforced specimens were shown to have a statistically significantly higher breaking strength than unreinforced controls after 1‐ and 2‐day hydration.
Conclusions
Reinforcement of liquid albumin solders in laser‐assisted incision repair appears to have advantages over conventional methods that do not reinforce the cohesive strength of the solder in terms of acute breaking strength and after immersion in moist environments for short periods of time. Using a film with the solder applied to one surface only may be advantageous over other techniques. Lasers Surg. Med. 28:297–306, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11344508</pmid><doi>10.1002/lsm.1053</doi><tpages>10</tpages></addata></record> |
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| ispartof | Lasers in surgery and medicine, 2001-01, Vol.28 (4), p.297-306 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | albumin Animals Aorta, Thoracic - surgery Biocompatible Materials Biodegradation, Environmental Biological and medical sciences Cattle diode laser Diseases of the cardiovascular system failure modes In Vitro Techniques Lactic Acid Laser Coagulation Medical sciences Microscopy, Electron, Scanning Miscellaneous poly-(lactic-co-glycolic acid) Polyglycolic Acid Polymers Prostheses and Implants Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Serum Albumin, Bovine Tensile Strength tissue welding |
| title | Laser-tissue soldering with biodegradable polymer films in vitro: Film surface morphology and hydration effects |
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