Imaging of QDs-labeled tumors in small animals by fluorescence diffuse tomography
Quantum dots (QD) are a new class of fluorescence labels with properties and biomedical applications that are not available with traditional organic dyes and fluorescent proteins. The unique optical properties of QDs (high absorption, quantum yield and emission spanning to near IR) make them attract...
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| Veröffentlicht in: | Laser physics letters 2006-04, Vol.3 (4), p.208-211 |
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| creator | Turchin, I. V. Balalaeva, I. V. Vasil'ev, R. B. Zlomanov, V. P. Plehanov, V. I. Orlova, A. G. Zagaynova, E. V. Kamensky, V. A. Kleshnin, M. S. Shirmanova, M. V. Dorofeev, S. G. Dirin, D. N. |
| description | Quantum dots (QD) are a new class of fluorescence labels with properties and biomedical applications that are not available with traditional organic dyes and fluorescent proteins. The unique optical properties of QDs (high absorption, quantum yield and emission spanning to near IR) make them attractive as in vivo markers for deep‐tissue imaging. Different types of optical tomography allow imaging of fluorescent‐labeled tumors located deep in human or animal tissue. In this work, preliminary results of the fluorescent diffuse tomography (FDT) method in application to CdTe/CdSe‐core/shell fluorescing nanocrystals are reported. In our experiments we utilized low‐frequency amplitude modulation (1 kHz) of the second harmonic of Nd:YAG laser (532 nm). The transilluminative configuration was used in the setup. Results of post mortem experiments with capsules containing QDs placed into esophagus of small animals to simulate labeled tumor are shown. An algorithm of processing fluorescent image based on calculating zero of maximum curvature has been applied to detect fluorescent inclusions boundaries on the image. This work demonstrates potential capabilities of the FDT method for imaging of deep fluorescent tumors in human tissue or animal models of human cancer. (© 2006 by Astro, Ltd. Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA) |
| doi_str_mv | 10.1002/lapl.200510086 |
| format | Article |
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In this work, preliminary results of the fluorescent diffuse tomography (FDT) method in application to CdTe/CdSe‐core/shell fluorescing nanocrystals are reported. In our experiments we utilized low‐frequency amplitude modulation (1 kHz) of the second harmonic of Nd:YAG laser (532 nm). The transilluminative configuration was used in the setup. Results of post mortem experiments with capsules containing QDs placed into esophagus of small animals to simulate labeled tumor are shown. An algorithm of processing fluorescent image based on calculating zero of maximum curvature has been applied to detect fluorescent inclusions boundaries on the image. This work demonstrates potential capabilities of the FDT method for imaging of deep fluorescent tumors in human tissue or animal models of human cancer. (© 2006 by Astro, Ltd. Published exclusively by WILEY‐VCH Verlag GmbH & Co. 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| identifier | ISSN: 1612-2011 |
| ispartof | Laser physics letters, 2006-04, Vol.3 (4), p.208-211 |
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| language | eng |
| recordid | cdi_crossref_primary_10_1002_lapl_200510086 |
| source | Institute of Physics Journals; Wiley Blackwell Single Titles |
| subjects | fluorescence diffuse tomography whole-body imaging |
| title | Imaging of QDs-labeled tumors in small animals by fluorescence diffuse tomography |
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