In vivo evaluation of garlic (Allium sativum) supplementation to rice straw-based diet on mitigation of CH 4 and CO 2 emissions and blood profiles using crossbreed rams

Two follow-up experiments (exp.) implemented using three cannulated crossbreed rams (Corriedale×Suffolk) fed rice straw based diet. In vivo evaluation of garlic powder (GP) supplementation to rice straw based diet on CH and CO emissions and blood profiles of the rams (BW = 52 ± 3 kg) was performed. The design was a triplicate 3×3 Latin Square. Groups were the control (no GP) and the two GP groups, which were given supplementation for 0.1 (GP0.1) and 0.5% (GP0.5) of concentrate. The differences between two exps. were data missing of GP0.1 and uncontrolled environment in exp. 1. Blood profiles were added in exp. 2. In exp. 1, supplementation of GP to the rams reduced CH production significantly (P < 0.05) by 38%. Dry matter intake (DMI) was not different among the groups in both exps. In exp. 2, CH was lower in GP0.5 group by 7% than the control group, whereas CO was not different among the groups. Serum glutamic oxaloacetic transaminase (GOT) was significantly (P > 0.05) lower in the GP0.5 group than the control group, whereas glutamic pyruvic transaminase (GPT), total protein (TP), urea N (UN) total cholesterol (T-CHO), low density lipoprotein (LDL), calcium (Ca), phosphorus (P), magnesium (Mg), and non-esterified fatty acids (NEFA) were not significantly (P > 0.05) different. Garlic powder supplementation, particularly GP0.5, decreases CH emissions in rams fed with rice straw based diets.