Synergy of tendon stem cells and platelet-rich plasma in tendon healing
Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate‐buffered saline (PBS), platelet‐rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protei...
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| Veröffentlicht in: | Journal of orthopaedic research 2012-06, Vol.30 (6), p.991-997 |
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| creator | Chen, Lei Dong, Shi-Wu Liu, Jun-Peng Tao, Xu Tang, Kang-Lai Xu, Jian-Zhong |
| description | Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate‐buffered saline (PBS), platelet‐rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP‐treated loaded tendons compared to PBS‐treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon‐healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:991–997, 2012 |
| doi_str_mv | 10.1002/jor.22033 |
| format | Article |
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Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP‐treated loaded tendons compared to PBS‐treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon‐healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:991–997, 2012</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.22033</identifier><identifier>PMID: 22161871</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Achilles Tendon - cytology ; Achilles Tendon - drug effects ; Achilles Tendon - physiopathology ; Animals ; Botulinum Toxins - pharmacology ; Cell- and Tissue-Based Therapy ; collagen type I ; Collagen Type I - genetics ; Collagen Type I - metabolism ; collagen type III ; Collagen Type III - genetics ; Collagen Type III - metabolism ; collagen/biosynthesis ; Culture Media - pharmacology ; Disease Models, Animal ; Female ; Gene Expression ; Platelet-Rich Plasma - physiology ; Rats ; Rats, Sprague-Dawley ; Smad8 Protein - genetics ; Smad8 Protein - metabolism ; Stem Cell Transplantation - methods ; Stem Cells - cytology ; Stem Cells - drug effects ; Stress, Mechanical ; Tenascin - genetics ; Tenascin - metabolism ; tenascin C ; Tendon Injuries - pathology ; Tendon Injuries - physiopathology ; Tendon Injuries - therapy ; Weight-Bearing ; Wound Healing - drug effects ; Wound Healing - genetics</subject><ispartof>Journal of orthopaedic research, 2012-06, Vol.30 (6), p.991-997</ispartof><rights>Copyright © 2011 Orthopaedic Research Society</rights><rights>Copyright © 2011 Orthopaedic Research Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3623-c44fbba61b0b50e0b1360c02dcb628e4cceb37e5c47d76a0fd056a1f3b3ad4e03</citedby><cites>FETCH-LOGICAL-c3623-c44fbba61b0b50e0b1360c02dcb628e4cceb37e5c47d76a0fd056a1f3b3ad4e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjor.22033$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjor.22033$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22161871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Dong, Shi-Wu</creatorcontrib><creatorcontrib>Liu, Jun-Peng</creatorcontrib><creatorcontrib>Tao, Xu</creatorcontrib><creatorcontrib>Tang, Kang-Lai</creatorcontrib><creatorcontrib>Xu, Jian-Zhong</creatorcontrib><title>Synergy of tendon stem cells and platelet-rich plasma in tendon healing</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate‐buffered saline (PBS), platelet‐rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP‐treated loaded tendons compared to PBS‐treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon‐healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:991–997, 2012</description><subject>Achilles Tendon - cytology</subject><subject>Achilles Tendon - drug effects</subject><subject>Achilles Tendon - physiopathology</subject><subject>Animals</subject><subject>Botulinum Toxins - pharmacology</subject><subject>Cell- and Tissue-Based Therapy</subject><subject>collagen type I</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>collagen type III</subject><subject>Collagen Type III - genetics</subject><subject>Collagen Type III - metabolism</subject><subject>collagen/biosynthesis</subject><subject>Culture Media - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Platelet-Rich Plasma - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Smad8 Protein - genetics</subject><subject>Smad8 Protein - metabolism</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stress, Mechanical</subject><subject>Tenascin - genetics</subject><subject>Tenascin - metabolism</subject><subject>tenascin C</subject><subject>Tendon Injuries - pathology</subject><subject>Tendon Injuries - physiopathology</subject><subject>Tendon Injuries - therapy</subject><subject>Weight-Bearing</subject><subject>Wound Healing - drug effects</subject><subject>Wound Healing - genetics</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMlOwzAQQC0EomU58AMoVw5px3Zi94oKbUEVRSyCm2U7kzYlS2UHQf6elNDeOI1GevOkeYRcUBhQADZcV27AGHB-QPo0jqMwZvL9kPRBchECE6JHTrxfA4CkbHRMeoxRQUeS9sn0uSnRLZugSoMay6QqA19jEVjMcx_oMgk2ua4xxzp0mV1tN1_oICt39Ap1npXLM3KU6tzj-d88Ja-T25fxLJwvpnfj63louWA8tFGUGqMFNWBiQDCUC7DAEmsEG2FkLRouMbaRTKTQkCYQC01TbrhOIgR-Sq46r3WV9w5TtXFZoV2jKKhtDNXGUL8xWvayYzefpsBkT-6-b4FhB3xlOTb_m9T94mmnDLuLrK30vb_Q7kMJyWWs3h6mit_A7HHeWib8B-UceG8</recordid><startdate>201206</startdate><enddate>201206</enddate><creator>Chen, Lei</creator><creator>Dong, Shi-Wu</creator><creator>Liu, Jun-Peng</creator><creator>Tao, Xu</creator><creator>Tang, Kang-Lai</creator><creator>Xu, Jian-Zhong</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201206</creationdate><title>Synergy of tendon stem cells and platelet-rich plasma in tendon healing</title><author>Chen, Lei ; Dong, Shi-Wu ; Liu, Jun-Peng ; Tao, Xu ; Tang, Kang-Lai ; Xu, Jian-Zhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3623-c44fbba61b0b50e0b1360c02dcb628e4cceb37e5c47d76a0fd056a1f3b3ad4e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Achilles Tendon - cytology</topic><topic>Achilles Tendon - drug effects</topic><topic>Achilles Tendon - physiopathology</topic><topic>Animals</topic><topic>Botulinum Toxins - pharmacology</topic><topic>Cell- and Tissue-Based Therapy</topic><topic>collagen type I</topic><topic>Collagen Type I - genetics</topic><topic>Collagen Type I - metabolism</topic><topic>collagen type III</topic><topic>Collagen Type III - genetics</topic><topic>Collagen Type III - metabolism</topic><topic>collagen/biosynthesis</topic><topic>Culture Media - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Platelet-Rich Plasma - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Smad8 Protein - genetics</topic><topic>Smad8 Protein - metabolism</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stress, Mechanical</topic><topic>Tenascin - genetics</topic><topic>Tenascin - metabolism</topic><topic>tenascin C</topic><topic>Tendon Injuries - pathology</topic><topic>Tendon Injuries - physiopathology</topic><topic>Tendon Injuries - therapy</topic><topic>Weight-Bearing</topic><topic>Wound Healing - drug effects</topic><topic>Wound Healing - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Dong, Shi-Wu</creatorcontrib><creatorcontrib>Liu, Jun-Peng</creatorcontrib><creatorcontrib>Tao, Xu</creatorcontrib><creatorcontrib>Tang, Kang-Lai</creatorcontrib><creatorcontrib>Xu, Jian-Zhong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Lei</au><au>Dong, Shi-Wu</au><au>Liu, Jun-Peng</au><au>Tao, Xu</au><au>Tang, Kang-Lai</au><au>Xu, Jian-Zhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergy of tendon stem cells and platelet-rich plasma in tendon healing</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2012-06</date><risdate>2012</risdate><volume>30</volume><issue>6</issue><spage>991</spage><epage>997</epage><pages>991-997</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate‐buffered saline (PBS), platelet‐rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP‐treated loaded tendons compared to PBS‐treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon‐healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:991–997, 2012</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22161871</pmid><doi>10.1002/jor.22033</doi><tpages>7</tpages></addata></record> |
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| subjects | Achilles Tendon - cytology Achilles Tendon - drug effects Achilles Tendon - physiopathology Animals Botulinum Toxins - pharmacology Cell- and Tissue-Based Therapy collagen type I Collagen Type I - genetics Collagen Type I - metabolism collagen type III Collagen Type III - genetics Collagen Type III - metabolism collagen/biosynthesis Culture Media - pharmacology Disease Models, Animal Female Gene Expression Platelet-Rich Plasma - physiology Rats Rats, Sprague-Dawley Smad8 Protein - genetics Smad8 Protein - metabolism Stem Cell Transplantation - methods Stem Cells - cytology Stem Cells - drug effects Stress, Mechanical Tenascin - genetics Tenascin - metabolism tenascin C Tendon Injuries - pathology Tendon Injuries - physiopathology Tendon Injuries - therapy Weight-Bearing Wound Healing - drug effects Wound Healing - genetics |
| title | Synergy of tendon stem cells and platelet-rich plasma in tendon healing |
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