Mechanosensitivity of voltage-gated K+ currents in rat trigeminal ganglion neurons
We investigated the mechanosensitivity of voltage‐gated K+ channel (VGPC) currents by using whole‐cell patch clamp recording in rat trigeminal ganglion (TG) neurons. On the basis of biophysical and pharmacological properties, two types of VGPC currents were isolated. One was transient (IK,A), the ot...
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| Veröffentlicht in: | Journal of neuroscience research 2006-05, Vol.83 (7), p.1373-1380 |
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| creator | Piao, Lin Ying Li, Hai Park, Chul-Kyu Cho, Ik-Hyun Piao, Zheng Gen Jung, Sung Jun Choi, Se-Young Lee, Sung Joong Park, Kyungpyo Kim, Joong-Soo Oh, Seog Bae |
| description | We investigated the mechanosensitivity of voltage‐gated K+ channel (VGPC) currents by using whole‐cell patch clamp recording in rat trigeminal ganglion (TG) neurons. On the basis of biophysical and pharmacological properties, two types of VGPC currents were isolated. One was transient (IK,A), the other sustained (IK,V). Hypotonic stimulation (200 mOsm) markedly increased both IK,A and IK,V without affecting their activation and inactivation kinetics. Gadolinium, a well‐known blocker of mechanosensitive channels, failed to block the enhancement of IK,A and IK,V induced by hypotonic stimulation. During hypotonic stimulation, cytochalasin D, an actin‐based cytoskeletal disruptor, further increased IK,A and IK,V, whereas phalloidin, an actin‐based cytoskeletal stabilizer, reduced IK,A and IK,V. Confocal imaging with Texas red‐phalloidin showed that actin‐based cytoskeleton was disrupted by hypotonic stimulation, which was similar to the effect of cytochalasin D. Our results suggest that both IK,A and IK,V are mechanosensitive and that actin‐based cytoskeleton is likely to regulate the mechanosensitivity of VGPC currents in TG neurons. © 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.20810 |
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On the basis of biophysical and pharmacological properties, two types of VGPC currents were isolated. One was transient (IK,A), the other sustained (IK,V). Hypotonic stimulation (200 mOsm) markedly increased both IK,A and IK,V without affecting their activation and inactivation kinetics. Gadolinium, a well‐known blocker of mechanosensitive channels, failed to block the enhancement of IK,A and IK,V induced by hypotonic stimulation. During hypotonic stimulation, cytochalasin D, an actin‐based cytoskeletal disruptor, further increased IK,A and IK,V, whereas phalloidin, an actin‐based cytoskeletal stabilizer, reduced IK,A and IK,V. Confocal imaging with Texas red‐phalloidin showed that actin‐based cytoskeleton was disrupted by hypotonic stimulation, which was similar to the effect of cytochalasin D. Our results suggest that both IK,A and IK,V are mechanosensitive and that actin‐based cytoskeleton is likely to regulate the mechanosensitivity of VGPC currents in TG neurons. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.20810</identifier><identifier>PMID: 16493687</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>actin ; Actin Cytoskeleton - drug effects ; Actin Cytoskeleton - metabolism ; Animals ; Animals, Newborn ; Cells, Cultured ; Cytochalasin D - pharmacology ; cytoskeleton ; Hypotonic Solutions - pharmacology ; hypotonic stimulation ; Mechanoreceptors - metabolism ; mechanosensitivity ; Mechanotransduction, Cellular - drug effects ; Mechanotransduction, Cellular - physiology ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Microscopy, Confocal ; Neurons, Afferent - cytology ; Neurons, Afferent - drug effects ; Neurons, Afferent - metabolism ; Nucleic Acid Synthesis Inhibitors - pharmacology ; Patch-Clamp Techniques ; Phalloidine - pharmacology ; Potassium Channel Blockers - pharmacology ; Potassium Channels, Voltage-Gated - drug effects ; Potassium Channels, Voltage-Gated - metabolism ; Rats ; Rats, Sprague-Dawley ; Trigeminal Ganglion - cytology ; Trigeminal Ganglion - drug effects ; Trigeminal Ganglion - metabolism ; trigeminal ganglion neuron ; voltage-gated K+ currents ; Xanthenes</subject><ispartof>Journal of neuroscience research, 2006-05, Vol.83 (7), p.1373-1380</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><rights>Copyright 2006 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4270-49831c3daa4571ce926cab7c63f845d06a790d616910e8e51b607f6f19172d3c3</citedby><cites>FETCH-LOGICAL-c4270-49831c3daa4571ce926cab7c63f845d06a790d616910e8e51b607f6f19172d3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.20810$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.20810$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16493687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Piao, Lin</creatorcontrib><creatorcontrib>Ying Li, Hai</creatorcontrib><creatorcontrib>Park, Chul-Kyu</creatorcontrib><creatorcontrib>Cho, Ik-Hyun</creatorcontrib><creatorcontrib>Piao, Zheng Gen</creatorcontrib><creatorcontrib>Jung, Sung Jun</creatorcontrib><creatorcontrib>Choi, Se-Young</creatorcontrib><creatorcontrib>Lee, Sung Joong</creatorcontrib><creatorcontrib>Park, Kyungpyo</creatorcontrib><creatorcontrib>Kim, Joong-Soo</creatorcontrib><creatorcontrib>Oh, Seog Bae</creatorcontrib><title>Mechanosensitivity of voltage-gated K+ currents in rat trigeminal ganglion neurons</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>We investigated the mechanosensitivity of voltage‐gated K+ channel (VGPC) currents by using whole‐cell patch clamp recording in rat trigeminal ganglion (TG) neurons. On the basis of biophysical and pharmacological properties, two types of VGPC currents were isolated. One was transient (IK,A), the other sustained (IK,V). Hypotonic stimulation (200 mOsm) markedly increased both IK,A and IK,V without affecting their activation and inactivation kinetics. Gadolinium, a well‐known blocker of mechanosensitive channels, failed to block the enhancement of IK,A and IK,V induced by hypotonic stimulation. During hypotonic stimulation, cytochalasin D, an actin‐based cytoskeletal disruptor, further increased IK,A and IK,V, whereas phalloidin, an actin‐based cytoskeletal stabilizer, reduced IK,A and IK,V. Confocal imaging with Texas red‐phalloidin showed that actin‐based cytoskeleton was disrupted by hypotonic stimulation, which was similar to the effect of cytochalasin D. Our results suggest that both IK,A and IK,V are mechanosensitive and that actin‐based cytoskeleton is likely to regulate the mechanosensitivity of VGPC currents in TG neurons. © 2006 Wiley‐Liss, Inc.</description><subject>actin</subject><subject>Actin Cytoskeleton - drug effects</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Cells, Cultured</subject><subject>Cytochalasin D - pharmacology</subject><subject>cytoskeleton</subject><subject>Hypotonic Solutions - pharmacology</subject><subject>hypotonic stimulation</subject><subject>Mechanoreceptors - metabolism</subject><subject>mechanosensitivity</subject><subject>Mechanotransduction, Cellular - drug effects</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Microscopy, Confocal</subject><subject>Neurons, Afferent - cytology</subject><subject>Neurons, Afferent - drug effects</subject><subject>Neurons, Afferent - metabolism</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Phalloidine - pharmacology</subject><subject>Potassium Channel Blockers - pharmacology</subject><subject>Potassium Channels, Voltage-Gated - drug effects</subject><subject>Potassium Channels, Voltage-Gated - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Trigeminal Ganglion - cytology</subject><subject>Trigeminal Ganglion - drug effects</subject><subject>Trigeminal Ganglion - metabolism</subject><subject>trigeminal ganglion neuron</subject><subject>voltage-gated K+ currents</subject><subject>Xanthenes</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAURi0EouUx8AeQV4QC13FixyMqUB6lSBUPicVyHSe4pA6yXaD_nkILTEx3Od_R1UFoj8ARAUiPJ84fpVAQWENdAoInWZ7xddQFyiDJgKQdtBXCBACEyOkm6hCWCcoK3kWjG6OflWuDccFG-2bjHLcVfmubqGqT1CqaEl8fYj3z3rgYsHXYq4ijt7WZWqcaXCtXN7Z12JmZb13YQRuVaoLZXd1tdH9-dte7SAa3_cveySDRWcoXf4mCEk1LpbKcE21EyrQac81oVWR5CUxxASUjTBAwhcnJmAGvWEUE4WlJNd1GB0uv9m0I3lTy1dup8nNJQH51kYsu8rvLgt1fsq-z8dSUf-QqxAI4XgLvtjHz_03yajj6USbLhQ3RfPwulH-RjFOey8dhXz4O-qe9h6ehvKGfuUJ8RQ</recordid><startdate>20060515</startdate><enddate>20060515</enddate><creator>Piao, Lin</creator><creator>Ying Li, Hai</creator><creator>Park, Chul-Kyu</creator><creator>Cho, Ik-Hyun</creator><creator>Piao, Zheng Gen</creator><creator>Jung, Sung Jun</creator><creator>Choi, Se-Young</creator><creator>Lee, Sung Joong</creator><creator>Park, Kyungpyo</creator><creator>Kim, Joong-Soo</creator><creator>Oh, Seog Bae</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20060515</creationdate><title>Mechanosensitivity of voltage-gated K+ currents in rat trigeminal ganglion neurons</title><author>Piao, Lin ; Ying Li, Hai ; Park, Chul-Kyu ; Cho, Ik-Hyun ; Piao, Zheng Gen ; Jung, Sung Jun ; Choi, Se-Young ; Lee, Sung Joong ; Park, Kyungpyo ; Kim, Joong-Soo ; Oh, Seog Bae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4270-49831c3daa4571ce926cab7c63f845d06a790d616910e8e51b607f6f19172d3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>actin</topic><topic>Actin Cytoskeleton - drug effects</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Cells, Cultured</topic><topic>Cytochalasin D - pharmacology</topic><topic>cytoskeleton</topic><topic>Hypotonic Solutions - pharmacology</topic><topic>hypotonic stimulation</topic><topic>Mechanoreceptors - metabolism</topic><topic>mechanosensitivity</topic><topic>Mechanotransduction, Cellular - drug effects</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Microscopy, Confocal</topic><topic>Neurons, Afferent - cytology</topic><topic>Neurons, Afferent - drug effects</topic><topic>Neurons, Afferent - metabolism</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacology</topic><topic>Patch-Clamp Techniques</topic><topic>Phalloidine - pharmacology</topic><topic>Potassium Channel Blockers - pharmacology</topic><topic>Potassium Channels, Voltage-Gated - drug effects</topic><topic>Potassium Channels, Voltage-Gated - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Trigeminal Ganglion - cytology</topic><topic>Trigeminal Ganglion - drug effects</topic><topic>Trigeminal Ganglion - metabolism</topic><topic>trigeminal ganglion neuron</topic><topic>voltage-gated K+ currents</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Piao, Lin</creatorcontrib><creatorcontrib>Ying Li, Hai</creatorcontrib><creatorcontrib>Park, Chul-Kyu</creatorcontrib><creatorcontrib>Cho, Ik-Hyun</creatorcontrib><creatorcontrib>Piao, Zheng Gen</creatorcontrib><creatorcontrib>Jung, Sung Jun</creatorcontrib><creatorcontrib>Choi, Se-Young</creatorcontrib><creatorcontrib>Lee, Sung Joong</creatorcontrib><creatorcontrib>Park, Kyungpyo</creatorcontrib><creatorcontrib>Kim, Joong-Soo</creatorcontrib><creatorcontrib>Oh, Seog Bae</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Piao, Lin</au><au>Ying Li, Hai</au><au>Park, Chul-Kyu</au><au>Cho, Ik-Hyun</au><au>Piao, Zheng Gen</au><au>Jung, Sung Jun</au><au>Choi, Se-Young</au><au>Lee, Sung Joong</au><au>Park, Kyungpyo</au><au>Kim, Joong-Soo</au><au>Oh, Seog Bae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanosensitivity of voltage-gated K+ currents in rat trigeminal ganglion neurons</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>2006-05-15</date><risdate>2006</risdate><volume>83</volume><issue>7</issue><spage>1373</spage><epage>1380</epage><pages>1373-1380</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><abstract>We investigated the mechanosensitivity of voltage‐gated K+ channel (VGPC) currents by using whole‐cell patch clamp recording in rat trigeminal ganglion (TG) neurons. On the basis of biophysical and pharmacological properties, two types of VGPC currents were isolated. One was transient (IK,A), the other sustained (IK,V). Hypotonic stimulation (200 mOsm) markedly increased both IK,A and IK,V without affecting their activation and inactivation kinetics. Gadolinium, a well‐known blocker of mechanosensitive channels, failed to block the enhancement of IK,A and IK,V induced by hypotonic stimulation. During hypotonic stimulation, cytochalasin D, an actin‐based cytoskeletal disruptor, further increased IK,A and IK,V, whereas phalloidin, an actin‐based cytoskeletal stabilizer, reduced IK,A and IK,V. Confocal imaging with Texas red‐phalloidin showed that actin‐based cytoskeleton was disrupted by hypotonic stimulation, which was similar to the effect of cytochalasin D. Our results suggest that both IK,A and IK,V are mechanosensitive and that actin‐based cytoskeleton is likely to regulate the mechanosensitivity of VGPC currents in TG neurons. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16493687</pmid><doi>10.1002/jnr.20810</doi><tpages>8</tpages></addata></record> |
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| subjects | actin Actin Cytoskeleton - drug effects Actin Cytoskeleton - metabolism Animals Animals, Newborn Cells, Cultured Cytochalasin D - pharmacology cytoskeleton Hypotonic Solutions - pharmacology hypotonic stimulation Mechanoreceptors - metabolism mechanosensitivity Mechanotransduction, Cellular - drug effects Mechanotransduction, Cellular - physiology Membrane Potentials - drug effects Membrane Potentials - physiology Microscopy, Confocal Neurons, Afferent - cytology Neurons, Afferent - drug effects Neurons, Afferent - metabolism Nucleic Acid Synthesis Inhibitors - pharmacology Patch-Clamp Techniques Phalloidine - pharmacology Potassium Channel Blockers - pharmacology Potassium Channels, Voltage-Gated - drug effects Potassium Channels, Voltage-Gated - metabolism Rats Rats, Sprague-Dawley Trigeminal Ganglion - cytology Trigeminal Ganglion - drug effects Trigeminal Ganglion - metabolism trigeminal ganglion neuron voltage-gated K+ currents Xanthenes |
| title | Mechanosensitivity of voltage-gated K+ currents in rat trigeminal ganglion neurons |
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