Simple single-step single-enzyme synthesis of [14C]-GSH

The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant...

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Veröffentlicht in:	Journal of labelled compounds & radiopharmaceuticals 2010-03, Vol.53 (3), p.110-112
Hauptverfasser:	de Keczer, Steve A., Voronin, Tatyana, Yao, Jennifer, Zhang, Fang-Jie, Masjedizadeh, Mohammad R.
Format:	Artikel
Sprache:	eng
Schlagworte:	[14C]-glutathione [14C]-glycine [14C]-GSH Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Contrast media. Radiopharmaceuticals Fundamental and applied biological sciences. Psychology GSH synthetase L-γ-glutamyl-L-cysteine Medical sciences Methods. Procedures. Technologies Pharmacology. Drug treatments
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Beschreibung
Zusammenfassung:	The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd. An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:	0362-4803 1099-1344
DOI:	10.1002/jlcr.1735