Preparation of individual electrically and video-recorded eggs for integrated temporal and electron microscopic analyses

A method for correlative studies of early fertilization events that integrates techniques of intracellular electrophysiological recording, video‐imaging, and electron microscopy is described. A key feature of the method is its ability to identify the fertilizing sperm and to record the moment of egg...

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Veröffentlicht in:	Microscopy research and technique 1992-02, Vol.20 (3), p.298-304
Hauptverfasser:	Longo, Frank J., McCulloh, David H., Ivonnet, Pedro I., Chambers, Edward L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Egg activation Electrophysiology Female Fertilization Fertilization in Vitro - methods Gamete fusion Male Microscopy, Electron - methods Ovum - ultrastructure Sea Urchins Sperm-egg interaction Sperm-Ovum Interactions - physiology Videotape Recording - methods
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