The use of the F.p.l.c. R system in method development and process monitoring for industrial protein chromatography
The f.p.l.c. R for high performance biomolecular purification is now widely used in laboratory and pilot scale separations, both as a means of developing new methods for purification and for analysing the purity of intermediary and final products. The speed of the technique means that it is possible...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 1986-08, Vol.36 (8), p.345-350 |
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| Format: | Artikel |
| Sprache: | eng |
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| container_end_page | 350 |
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| container_issue | 8 |
| container_start_page | 345 |
| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 36 |
| creator | Low, D. K. R. |
| description | The f.p.l.c.
R
for high performance biomolecular purification is now widely used in laboratory and pilot scale separations, both as a means of developing new methods for purification and for analysing the purity of intermediary and final products. The speed of the technique means that it is possible to analyse fractions from a process step and to receive the results within 15–30 min. Two aspects are examined in this paper: the monitoring of protein product formation during fermentation and the analysis of fractions in large scale chromatography. The suitability of the technique in an operating process is discussed. |
| doi_str_mv | 10.1002/jctb.280360802 |
| format | Article |
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| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 1986-08, Vol.36 (8), p.345-350 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_jctb_280360802 |
| source | Wiley Online Library Journals Frontfile Complete |
| title | The use of the F.p.l.c. R system in method development and process monitoring for industrial protein chromatography |
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