Isolation of baculovirus-expressed human vitamin D receptor: DNA responsive element interactions and phosphorylation of the purified receptor

Two controversial aspects in the mechanism of human vitamin D receptor (hVDR) action are the possible significance of VDR homodimers and the functional role of receptor phosphorylation. To address these issues, milligram quantities of baculovirus‐expressed hVDR were purified to 97% homogeneity, and...

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Veröffentlicht in:	Journal of cellular biochemistry 2002, Vol.85 (2), p.435-457
Hauptverfasser:	Jurutka, Peter W., MacDonald, Paul N., Nakajima, Shigeo, Hsieh, Jui-Cheng, Thompson, Paul D, Whitfield, G. Kerr, Galligan, Michael A., Haussler, Carol A., Haussler, Mark R.
Format:	Artikel
Sprache:	eng
Schlagworte:	1,25‐dihydroxyvitamin D3 25-dihydroxyvitamin D3 Acid Phosphatase - metabolism Animals Baculoviridae - genetics Binding Sites Blotting, Western COS Cells Dimerization DNA - metabolism DNA Primers - chemistry Electrophoretic Mobility Shift Assay Genes, Regulator - genetics Genetic Vectors heterodimerization Humans nuclear hormone receptor Okadaic Acid - pharmacology Osteocalcin - genetics Osteocalcin - metabolism Phosphorylation Promoter Regions, Genetic - genetics Rats Receptors, Calcitriol - genetics Receptors, Calcitriol - isolation & purification Receptors, Calcitriol - metabolism Receptors, Retinoic Acid - genetics Recombinant Proteins - genetics Recombinant Proteins - metabolism Response Elements - genetics Retinoid X Receptors Transcription Factors - genetics transcriptional activation Tretinoin - physiology Vitamin D - metabolism
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