Melatonin inhibits the contractile effect of vanadate in the isolated pulmonary arterial rings of rats: Possible role of hydrogen peroxide
The effect and possible mechanism of action of vanadate on the isolated pulmonary arterial rings of normal rats were studied. Pulmonary arterial rings contracted in response to vanadate (0.1–1 mM) in a concentration‐dependent manner. Preincubation of the pulmonary arterial rings with 1 mM melatonin...
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| Veröffentlicht in: | Journal of biochemical and molecular toxicology 2002-01, Vol.16 (6), p.273-278 |
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| creator | Nagi, Mahmoud N. Mansour, Mahmoud A. Al-shabanah, Othman A. El-Kashef, Hassan A. |
| description | The effect and possible mechanism of action of vanadate on the isolated pulmonary arterial rings of normal rats were studied. Pulmonary arterial rings contracted in response to vanadate (0.1–1 mM) in a concentration‐dependent manner. Preincubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile effect of vanadate by more than 60%. Furthermore, addition of hydrogen peroxide (50 μM) or enzymatic generation of hydrogen peroxide by the addition of glucose oxidase (10 U/mL) to the medium containing glucose produced remarkable increases in the pulmonary arterial tension, 46.2 ± 7.3 and 78.7 ± 9.7 g tension/g tissue, respectively. Similarly, incubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile responses of the arterial rings to hydrogen peroxide and glucose/glucose oxidase to 25.7 ± 2.9 and 24.7 ± 4.4 g tension/g tissue, respectively. Vanadate, in vitro, significantly stimulated the oxidation of NADH by xanthine oxidase, and the rate of oxidation was increased by increasing either time or vanadate concentration. Similarly, addition of melatonin to a reaction mixture containing xanthine oxidase and vanadate significantly inhibited the rate of NADH oxidation in a concentration‐dependent fashion. The results of the present study indicated that vanadate induced contraction in the isolated pulmonary arterial rings, which was significantly reduced by melatonin. Furthermore, the contractile effect of vanadate on the pulmonary arterial rings may be attributed to the intracellular generation of hydrogen peroxide. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:273–278, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10049 |
| doi_str_mv | 10.1002/jbt.10049 |
| format | Article |
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Pulmonary arterial rings contracted in response to vanadate (0.1–1 mM) in a concentration‐dependent manner. Preincubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile effect of vanadate by more than 60%. Furthermore, addition of hydrogen peroxide (50 μM) or enzymatic generation of hydrogen peroxide by the addition of glucose oxidase (10 U/mL) to the medium containing glucose produced remarkable increases in the pulmonary arterial tension, 46.2 ± 7.3 and 78.7 ± 9.7 g tension/g tissue, respectively. Similarly, incubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile responses of the arterial rings to hydrogen peroxide and glucose/glucose oxidase to 25.7 ± 2.9 and 24.7 ± 4.4 g tension/g tissue, respectively. Vanadate, in vitro, significantly stimulated the oxidation of NADH by xanthine oxidase, and the rate of oxidation was increased by increasing either time or vanadate concentration. Similarly, addition of melatonin to a reaction mixture containing xanthine oxidase and vanadate significantly inhibited the rate of NADH oxidation in a concentration‐dependent fashion. The results of the present study indicated that vanadate induced contraction in the isolated pulmonary arterial rings, which was significantly reduced by melatonin. Furthermore, the contractile effect of vanadate on the pulmonary arterial rings may be attributed to the intracellular generation of hydrogen peroxide. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:273–278, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10049</description><identifier>ISSN: 1095-6670</identifier><identifier>EISSN: 1099-0461</identifier><identifier>DOI: 10.1002/jbt.10049</identifier><identifier>PMID: 12481302</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Drug Interactions ; Glucose - metabolism ; Glucose Oxidase - metabolism ; Hydrogen Peroxide ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; In Vitro Techniques ; Kinetics ; Melatonin ; Melatonin - pharmacology ; Muscle Contraction - drug effects ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - physiology ; NAD - metabolism ; Oxidation-Reduction ; Pulmonary Arterial Rings ; Pulmonary Artery - drug effects ; Pulmonary Artery - physiology ; Rats ; Rats, Wistar ; Vanadate ; Vanadates - antagonists & inhibitors ; Vanadates - pharmacology ; Xanthine Oxidase ; Xanthine Oxidase - metabolism</subject><ispartof>Journal of biochemical and molecular toxicology, 2002-01, Vol.16 (6), p.273-278</ispartof><rights>Copyright © 2002 Wiley Periodicals, Inc.</rights><rights>Copyright 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:273-278, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10049</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3599-f04fcbddff49b37b5e065e1c45031cfdbc415606166931d930356bc34f8fef203</citedby><cites>FETCH-LOGICAL-c3599-f04fcbddff49b37b5e065e1c45031cfdbc415606166931d930356bc34f8fef203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbt.10049$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbt.10049$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12481302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagi, Mahmoud N.</creatorcontrib><creatorcontrib>Mansour, Mahmoud A.</creatorcontrib><creatorcontrib>Al-shabanah, Othman A.</creatorcontrib><creatorcontrib>El-Kashef, Hassan A.</creatorcontrib><title>Melatonin inhibits the contractile effect of vanadate in the isolated pulmonary arterial rings of rats: Possible role of hydrogen peroxide</title><title>Journal of biochemical and molecular toxicology</title><addtitle>J. Biochem. Mol. Toxicol</addtitle><description>The effect and possible mechanism of action of vanadate on the isolated pulmonary arterial rings of normal rats were studied. Pulmonary arterial rings contracted in response to vanadate (0.1–1 mM) in a concentration‐dependent manner. Preincubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile effect of vanadate by more than 60%. Furthermore, addition of hydrogen peroxide (50 μM) or enzymatic generation of hydrogen peroxide by the addition of glucose oxidase (10 U/mL) to the medium containing glucose produced remarkable increases in the pulmonary arterial tension, 46.2 ± 7.3 and 78.7 ± 9.7 g tension/g tissue, respectively. Similarly, incubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile responses of the arterial rings to hydrogen peroxide and glucose/glucose oxidase to 25.7 ± 2.9 and 24.7 ± 4.4 g tension/g tissue, respectively. Vanadate, in vitro, significantly stimulated the oxidation of NADH by xanthine oxidase, and the rate of oxidation was increased by increasing either time or vanadate concentration. Similarly, addition of melatonin to a reaction mixture containing xanthine oxidase and vanadate significantly inhibited the rate of NADH oxidation in a concentration‐dependent fashion. The results of the present study indicated that vanadate induced contraction in the isolated pulmonary arterial rings, which was significantly reduced by melatonin. Furthermore, the contractile effect of vanadate on the pulmonary arterial rings may be attributed to the intracellular generation of hydrogen peroxide. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:273–278, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10049</description><subject>Animals</subject><subject>Drug Interactions</subject><subject>Glucose - metabolism</subject><subject>Glucose Oxidase - metabolism</subject><subject>Hydrogen Peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Melatonin</subject><subject>Melatonin - pharmacology</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>NAD - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Pulmonary Arterial Rings</subject><subject>Pulmonary Artery - drug effects</subject><subject>Pulmonary Artery - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vanadate</subject><subject>Vanadates - antagonists & inhibitors</subject><subject>Vanadates - pharmacology</subject><subject>Xanthine Oxidase</subject><subject>Xanthine Oxidase - metabolism</subject><issn>1095-6670</issn><issn>1099-0461</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtuEzEUhi0EohdY8ALIWxbTHseXybCDClpQAyyKkLqxfDluXCbjyHaheQWeGicpsGJjH1nf_-v4I-QFgxMGMDu9tXU7iOEROWQwDB0IxR7vZtkp1cMBOSrlFgDk0Mun5IDNxJxxmB2SXwscTU1TnGicltHGWmhdInVpqtm4GkekGAK6SlOgP8xkvKnY2B0VS2pp9HR9N67SZPKGmlwxRzPSHKebsg1lU8tr-iWVEm1ry6kd7Xm58Tnd4ETXmNN99PiMPAlmLPj84T4mX9-_uzq76C4_n384e3PZOS7b3wKI4Kz3IYjB8t5KBCWROSGBMxe8dYJJBYopNXDmBw5cKuu4CPOAYQb8mLza97rcdsoY9DrHVdtdM9Bbn7r51DufjX25Z9d3doX-H_kgsAGne-BnM7X5f5P--PbqT2W3T8RS8f5vwuTvWvW8l_rbp3M9Xyyu--sLoRn_DeL1kVc</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Nagi, Mahmoud N.</creator><creator>Mansour, Mahmoud A.</creator><creator>Al-shabanah, Othman A.</creator><creator>El-Kashef, Hassan A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020101</creationdate><title>Melatonin inhibits the contractile effect of vanadate in the isolated pulmonary arterial rings of rats: Possible role of hydrogen peroxide</title><author>Nagi, Mahmoud N. ; Mansour, Mahmoud A. ; Al-shabanah, Othman A. ; El-Kashef, Hassan A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3599-f04fcbddff49b37b5e065e1c45031cfdbc415606166931d930356bc34f8fef203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Drug Interactions</topic><topic>Glucose - metabolism</topic><topic>Glucose Oxidase - metabolism</topic><topic>Hydrogen Peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Melatonin</topic><topic>Melatonin - pharmacology</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>NAD - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Pulmonary Arterial Rings</topic><topic>Pulmonary Artery - drug effects</topic><topic>Pulmonary Artery - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vanadate</topic><topic>Vanadates - antagonists & inhibitors</topic><topic>Vanadates - pharmacology</topic><topic>Xanthine Oxidase</topic><topic>Xanthine Oxidase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagi, Mahmoud N.</creatorcontrib><creatorcontrib>Mansour, Mahmoud A.</creatorcontrib><creatorcontrib>Al-shabanah, Othman A.</creatorcontrib><creatorcontrib>El-Kashef, Hassan A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of biochemical and molecular toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagi, Mahmoud N.</au><au>Mansour, Mahmoud A.</au><au>Al-shabanah, Othman A.</au><au>El-Kashef, Hassan A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin inhibits the contractile effect of vanadate in the isolated pulmonary arterial rings of rats: Possible role of hydrogen peroxide</atitle><jtitle>Journal of biochemical and molecular toxicology</jtitle><addtitle>J. Biochem. Mol. Toxicol</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>16</volume><issue>6</issue><spage>273</spage><epage>278</epage><pages>273-278</pages><issn>1095-6670</issn><eissn>1099-0461</eissn><abstract>The effect and possible mechanism of action of vanadate on the isolated pulmonary arterial rings of normal rats were studied. Pulmonary arterial rings contracted in response to vanadate (0.1–1 mM) in a concentration‐dependent manner. Preincubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile effect of vanadate by more than 60%. Furthermore, addition of hydrogen peroxide (50 μM) or enzymatic generation of hydrogen peroxide by the addition of glucose oxidase (10 U/mL) to the medium containing glucose produced remarkable increases in the pulmonary arterial tension, 46.2 ± 7.3 and 78.7 ± 9.7 g tension/g tissue, respectively. Similarly, incubation of the pulmonary arterial rings with 1 mM melatonin significantly reduced the contractile responses of the arterial rings to hydrogen peroxide and glucose/glucose oxidase to 25.7 ± 2.9 and 24.7 ± 4.4 g tension/g tissue, respectively. Vanadate, in vitro, significantly stimulated the oxidation of NADH by xanthine oxidase, and the rate of oxidation was increased by increasing either time or vanadate concentration. Similarly, addition of melatonin to a reaction mixture containing xanthine oxidase and vanadate significantly inhibited the rate of NADH oxidation in a concentration‐dependent fashion. The results of the present study indicated that vanadate induced contraction in the isolated pulmonary arterial rings, which was significantly reduced by melatonin. Furthermore, the contractile effect of vanadate on the pulmonary arterial rings may be attributed to the intracellular generation of hydrogen peroxide. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:273–278, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10049</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12481302</pmid><doi>10.1002/jbt.10049</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Drug Interactions Glucose - metabolism Glucose Oxidase - metabolism Hydrogen Peroxide Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology In Vitro Techniques Kinetics Melatonin Melatonin - pharmacology Muscle Contraction - drug effects Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - physiology NAD - metabolism Oxidation-Reduction Pulmonary Arterial Rings Pulmonary Artery - drug effects Pulmonary Artery - physiology Rats Rats, Wistar Vanadate Vanadates - antagonists & inhibitors Vanadates - pharmacology Xanthine Oxidase Xanthine Oxidase - metabolism |
| title | Melatonin inhibits the contractile effect of vanadate in the isolated pulmonary arterial rings of rats: Possible role of hydrogen peroxide |
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