Development of a reconstituted water medium and preliminary validation of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX)
A reconstituted water medium was developed for use in the Frog Embryo Teratogenesis Assay—Xenopus (FETAX). FETAX solution was then tested on three compounds with known mammalian teratogenicity (ethanol, caffeine, and 5‐fluorouracil) as was a non‐teratogen (saccharin). The results obtained were then...
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| Veröffentlicht in: | Journal of applied toxicology 1987-08, Vol.7 (4), p.237-244 |
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| container_title | Journal of applied toxicology |
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| creator | Dawson, Douglas A. Bantle, John A. |
| description | A reconstituted water medium was developed for use in the Frog Embryo Teratogenesis Assay—Xenopus (FETAX). FETAX solution was then tested on three compounds with known mammalian teratogenicity (ethanol, caffeine, and 5‐fluorouracil) as was a non‐teratogen (saccharin). The results obtained were then compared with results from tests on these compounds in two other media that had previously been used in the assay. Saccharin was not teratogenic. Ethanol and caffeine were weak and moderate teratogens, respectively. 5‐fluorouracil was a strong teratogen. The results compare favorably with those obtained in mammalian studies. The amount of growth inhibition in embryos in the 96 h tests was positively correlated with the degree of teratogenicity of the compound. Final validation of FETAX will allow it to be used to screen and rank compounds for further testing and as a tool for studying the basic mechanisms of teratogenesis. |
| doi_str_mv | 10.1002/jat.2550070403 |
| format | Article |
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FETAX solution was then tested on three compounds with known mammalian teratogenicity (ethanol, caffeine, and 5‐fluorouracil) as was a non‐teratogen (saccharin). The results obtained were then compared with results from tests on these compounds in two other media that had previously been used in the assay. Saccharin was not teratogenic. Ethanol and caffeine were weak and moderate teratogens, respectively. 5‐fluorouracil was a strong teratogen. The results compare favorably with those obtained in mammalian studies. The amount of growth inhibition in embryos in the 96 h tests was positively correlated with the degree of teratogenicity of the compound. 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Appl. Toxicol</addtitle><description>A reconstituted water medium was developed for use in the Frog Embryo Teratogenesis Assay—Xenopus (FETAX). FETAX solution was then tested on three compounds with known mammalian teratogenicity (ethanol, caffeine, and 5‐fluorouracil) as was a non‐teratogen (saccharin). The results obtained were then compared with results from tests on these compounds in two other media that had previously been used in the assay. Saccharin was not teratogenic. Ethanol and caffeine were weak and moderate teratogens, respectively. 5‐fluorouracil was a strong teratogen. The results compare favorably with those obtained in mammalian studies. The amount of growth inhibition in embryos in the 96 h tests was positively correlated with the degree of teratogenicity of the compound. Final validation of FETAX will allow it to be used to screen and rank compounds for further testing and as a tool for studying the basic mechanisms of teratogenesis.</description><subject>5-fluorouracil</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>caffeine</subject><subject>Caffeine - toxicity</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Chemical agents</subject><subject>Culture Media</subject><subject>ethanol</subject><subject>Ethanol - toxicity</subject><subject>FETAX</subject><subject>Fluorouracil - toxicity</subject><subject>Medical sciences</subject><subject>saccharin</subject><subject>Saccharin - toxicity</subject><subject>teratogenicity</subject><subject>Teratogens - toxicity</subject><subject>Tumors</subject><subject>Water</subject><subject>Xenopus</subject><issn>0260-437X</issn><issn>1099-1263</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1v2zAQxYmiReqkWbsV4NChHeRSJCVKo-F8N2mBwmm9ERR1TJlKokDSSTzkfw8NGy46FRxueO93vPcQep-TaU4I_XKv4pQWBSGCcMJeoUlO6jrLacleowmhJck4E8u36DCEe0KSRqsDdMBKykXFJuj5BB6gc2MPQ8TOYIU9aDeEaOMqQosfVQSPe2jtqsdqaPHoobO9HZRf4wfV2VZF64YNGn8DPvPuDp_2jV87vACvoruDAYINeBaCWmdLGNy4CvjT2elitvz8Dr0xqgtwvJtH6DYJ84vs-vv55Xx2nWkuGMt4XddVabQuTaWqOm8446TmpAFWCmNMQ41uoGkbnnNDVdFCW3BNSStSTF1RdoSm273auxA8GDl626cIMidyU6NMNcq_NSbgwxYYV00Kv7fvekv6x52uglad8WrQNuxtgpfpbWz11vZoO1j_51N5NVv8c0K2ZW2I8LRnlf8jS8FEIX99O5fF4qZa_pz_kF_ZCz8LnJg</recordid><startdate>198708</startdate><enddate>198708</enddate><creator>Dawson, Douglas A.</creator><creator>Bantle, John A.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198708</creationdate><title>Development of a reconstituted water medium and preliminary validation of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX)</title><author>Dawson, Douglas A. ; Bantle, John A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4733-499986fcc6f8a891b4340940be367fffb2fcbebdb414f2a5ded54c20d7624c823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>5-fluorouracil</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>caffeine</topic><topic>Caffeine - toxicity</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Chemical agents</topic><topic>Culture Media</topic><topic>ethanol</topic><topic>Ethanol - toxicity</topic><topic>FETAX</topic><topic>Fluorouracil - toxicity</topic><topic>Medical sciences</topic><topic>saccharin</topic><topic>Saccharin - toxicity</topic><topic>teratogenicity</topic><topic>Teratogens - toxicity</topic><topic>Tumors</topic><topic>Water</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dawson, Douglas A.</creatorcontrib><creatorcontrib>Bantle, John A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of applied toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dawson, Douglas A.</au><au>Bantle, John A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a reconstituted water medium and preliminary validation of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX)</atitle><jtitle>Journal of applied toxicology</jtitle><addtitle>J. Appl. Toxicol</addtitle><date>1987-08</date><risdate>1987</risdate><volume>7</volume><issue>4</issue><spage>237</spage><epage>244</epage><pages>237-244</pages><issn>0260-437X</issn><eissn>1099-1263</eissn><coden>JJATDK</coden><abstract>A reconstituted water medium was developed for use in the Frog Embryo Teratogenesis Assay—Xenopus (FETAX). FETAX solution was then tested on three compounds with known mammalian teratogenicity (ethanol, caffeine, and 5‐fluorouracil) as was a non‐teratogen (saccharin). The results obtained were then compared with results from tests on these compounds in two other media that had previously been used in the assay. Saccharin was not teratogenic. Ethanol and caffeine were weak and moderate teratogens, respectively. 5‐fluorouracil was a strong teratogen. The results compare favorably with those obtained in mammalian studies. The amount of growth inhibition in embryos in the 96 h tests was positively correlated with the degree of teratogenicity of the compound. Final validation of FETAX will allow it to be used to screen and rank compounds for further testing and as a tool for studying the basic mechanisms of teratogenesis.</abstract><cop>Chichester</cop><pub>John Wiley & Sons, Ltd</pub><pmid>3624783</pmid><doi>10.1002/jat.2550070403</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0260-437X |
| ispartof | Journal of applied toxicology, 1987-08, Vol.7 (4), p.237-244 |
| issn | 0260-437X 1099-1263 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_jat_2550070403 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 5-fluorouracil Animals Biological and medical sciences caffeine Caffeine - toxicity Carcinogenesis, carcinogens and anticarcinogens Chemical agents Culture Media ethanol Ethanol - toxicity FETAX Fluorouracil - toxicity Medical sciences saccharin Saccharin - toxicity teratogenicity Teratogens - toxicity Tumors Water Xenopus |
| title | Development of a reconstituted water medium and preliminary validation of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX) |
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