Stability of Aroclor 1254-induced rat liver postmitochondrial supernatant (S-9) following long-term storage (-75 degrees C)

The stability of Aroclor 1254‐induced rat liver postmitochondrial supernatant (S‐9) over a 5‐year period was investigated in a retrospective study. S‐9 was uniformly prepared at 6‐month intervals, and aliquots were stored at −75°C. The protein and cytochrome P‐450 content of these lots of S‐9 were v...

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Veröffentlicht in:Journal of applied toxicology 1985-06, Vol.5 (3), p.187-191
Hauptverfasser: Oldham, J.W, Pritchard, J.F, Preston, R.F, Nomides, C.T, Patton, W.E, Paulson, J.D
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container_end_page 191
container_issue 3
container_start_page 187
container_title Journal of applied toxicology
container_volume 5
creator Oldham, J.W
Pritchard, J.F
Preston, R.F
Nomides, C.T
Patton, W.E
Paulson, J.D
description The stability of Aroclor 1254‐induced rat liver postmitochondrial supernatant (S‐9) over a 5‐year period was investigated in a retrospective study. S‐9 was uniformly prepared at 6‐month intervals, and aliquots were stored at −75°C. The protein and cytochrome P‐450 content of these lots of S‐9 were very similar, and no differences attributable to duration of storage were observed in the activities of ethoxycoumarin O‐deethylase, aniline hydroxyiase, cytochrome P‐450 reductase or aryl hydrocarbon hydroxylase. There was no decrease following 5 years of storage in the ability of S‐9 to activate 2‐aminoanthracene, as measured in the Ames test (TA98), but there was a notable reduction following more than 1 year of storage in the ability of the S‐9 to generate Ames test activity with benzo(a)pyrene. Based on the results of these studies, S‐9 prepared and stored under these conditions appears to be suitable for use in in vitro genotoxicity assays for at least 1 year.
doi_str_mv 10.1002/jat.2550050310
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S‐9 was uniformly prepared at 6‐month intervals, and aliquots were stored at −75°C. The protein and cytochrome P‐450 content of these lots of S‐9 were very similar, and no differences attributable to duration of storage were observed in the activities of ethoxycoumarin O‐deethylase, aniline hydroxyiase, cytochrome P‐450 reductase or aryl hydrocarbon hydroxylase. There was no decrease following 5 years of storage in the ability of S‐9 to activate 2‐aminoanthracene, as measured in the Ames test (TA98), but there was a notable reduction following more than 1 year of storage in the ability of the S‐9 to generate Ames test activity with benzo(a)pyrene. Based on the results of these studies, S‐9 prepared and stored under these conditions appears to be suitable for use in in vitro genotoxicity assays for at least 1 year.</description><identifier>ISSN: 0260-437X</identifier><identifier>EISSN: 1099-1263</identifier><identifier>DOI: 10.1002/jat.2550050310</identifier><identifier>PMID: 3924988</identifier><identifier>CODEN: JJATDK</identifier><language>eng</language><publisher>Chichester: John Wiley &amp; Sons, Ltd</publisher><subject>2-aminoanthracene ; 7-Alkoxycoumarin O-Dealkylase ; activating system ; Ames test ; Aniline Hydroxylase - biosynthesis ; Animals ; arochlor ; Aroclors - pharmacology ; Aryl Hydrocarbon Hydroxylases - biosynthesis ; benzo(a)pyrene ; Biological and medical sciences ; Chemical and industrial products toxicology. Toxic occupational diseases ; Chlorodiphenyl (54% Chlorine) ; cytochrome P-450 ; Cytochrome P-450 Enzyme System - biosynthesis ; cytochromes ; Enzyme Induction ; Freezing ; frozen storage ; genotoxicity testing ; In Vitro Techniques ; liver ; Liver - metabolism ; Male ; Medical sciences ; mitochondria ; Mutagenicity Tests ; Mutagens ; Oxygenases - biosynthesis ; Polychlorinated Biphenyls - pharmacology ; Preservation, Biological ; Rats ; S-9 ; Salmonella typhimurium - genetics ; Specimen Handling ; stability ; Subcellular Fractions - metabolism ; Toxicology ; Various organic compounds</subject><ispartof>Journal of applied toxicology, 1985-06, Vol.5 (3), p.187-191</ispartof><rights>Copyright © 1985 John Wiley &amp; Sons, Ltd.</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4310-8ac29f033bc48d679fd47f131091c211a5ee52be976cddcccc7ce01775a9cd03</citedby><cites>FETCH-LOGICAL-c4310-8ac29f033bc48d679fd47f131091c211a5ee52be976cddcccc7ce01775a9cd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjat.2550050310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjat.2550050310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8516013$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3924988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oldham, J.W</creatorcontrib><creatorcontrib>Pritchard, J.F</creatorcontrib><creatorcontrib>Preston, R.F</creatorcontrib><creatorcontrib>Nomides, C.T</creatorcontrib><creatorcontrib>Patton, W.E</creatorcontrib><creatorcontrib>Paulson, J.D</creatorcontrib><title>Stability of Aroclor 1254-induced rat liver postmitochondrial supernatant (S-9) following long-term storage (-75 degrees C)</title><title>Journal of applied toxicology</title><addtitle>J. Appl. Toxicol</addtitle><description>The stability of Aroclor 1254‐induced rat liver postmitochondrial supernatant (S‐9) over a 5‐year period was investigated in a retrospective study. S‐9 was uniformly prepared at 6‐month intervals, and aliquots were stored at −75°C. The protein and cytochrome P‐450 content of these lots of S‐9 were very similar, and no differences attributable to duration of storage were observed in the activities of ethoxycoumarin O‐deethylase, aniline hydroxyiase, cytochrome P‐450 reductase or aryl hydrocarbon hydroxylase. There was no decrease following 5 years of storage in the ability of S‐9 to activate 2‐aminoanthracene, as measured in the Ames test (TA98), but there was a notable reduction following more than 1 year of storage in the ability of the S‐9 to generate Ames test activity with benzo(a)pyrene. Based on the results of these studies, S‐9 prepared and stored under these conditions appears to be suitable for use in in vitro genotoxicity assays for at least 1 year.</description><subject>2-aminoanthracene</subject><subject>7-Alkoxycoumarin O-Dealkylase</subject><subject>activating system</subject><subject>Ames test</subject><subject>Aniline Hydroxylase - biosynthesis</subject><subject>Animals</subject><subject>arochlor</subject><subject>Aroclors - pharmacology</subject><subject>Aryl Hydrocarbon Hydroxylases - biosynthesis</subject><subject>benzo(a)pyrene</subject><subject>Biological and medical sciences</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Chlorodiphenyl (54% Chlorine)</subject><subject>cytochrome P-450</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>cytochromes</subject><subject>Enzyme Induction</subject><subject>Freezing</subject><subject>frozen storage</subject><subject>genotoxicity testing</subject><subject>In Vitro Techniques</subject><subject>liver</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>mitochondria</subject><subject>Mutagenicity Tests</subject><subject>Mutagens</subject><subject>Oxygenases - biosynthesis</subject><subject>Polychlorinated Biphenyls - pharmacology</subject><subject>Preservation, Biological</subject><subject>Rats</subject><subject>S-9</subject><subject>Salmonella typhimurium - genetics</subject><subject>Specimen Handling</subject><subject>stability</subject><subject>Subcellular Fractions - metabolism</subject><subject>Toxicology</subject><subject>Various organic compounds</subject><issn>0260-437X</issn><issn>1099-1263</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM2PUyEUxYnRjHV0687IYhYzCyofj8dj2TQ6ahqd2KqzIxR4lZE-GqCOjf-8mNfUuJLNXZzfuZx7AHhO8JRgTF_d6TKlnGPMMSP4AZgQLCUitGUPwQTTFqOGidvH4EnOdxhXjXZn4IxJ2sium4Bfy6LXPvhygLGHsxRNiAkSyhvkB7s3zsKkCwz-h0twF3PZ-hLNtzjY5HWAeb9zadBFDwVeLpG8gn0MId77YQNDHDaouLSFucSkNw5eIsGhdZvkXIbzq6fgUa9Dds-O8xys3rxezd-ixcfrd_PZApmmnoQ6bajsMWNr03S2FbK3jehJlSQxlBDNneN07aRojbWmPmEcJkJwLY3F7BxMx7UmxZyT69Uu-a1OB0Ww-tOhqh2qvx1Ww4vRsNuvt86e8GNpVb846jobHfqkB-PzCes4aTFhFZMjdu-DO_znU_V-tvonAhq9Phf38-TV6btqBRNcff1wrfCX2xvyaXGjROVfjnyvo9KbVON8XtKaApOmbVvcsd9-y6K1</recordid><startdate>198506</startdate><enddate>198506</enddate><creator>Oldham, J.W</creator><creator>Pritchard, J.F</creator><creator>Preston, R.F</creator><creator>Nomides, C.T</creator><creator>Patton, W.E</creator><creator>Paulson, J.D</creator><general>John Wiley &amp; Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198506</creationdate><title>Stability of Aroclor 1254-induced rat liver postmitochondrial supernatant (S-9) following long-term storage (-75 degrees C)</title><author>Oldham, J.W ; Pritchard, J.F ; Preston, R.F ; Nomides, C.T ; Patton, W.E ; Paulson, J.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4310-8ac29f033bc48d679fd47f131091c211a5ee52be976cddcccc7ce01775a9cd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>2-aminoanthracene</topic><topic>7-Alkoxycoumarin O-Dealkylase</topic><topic>activating system</topic><topic>Ames test</topic><topic>Aniline Hydroxylase - biosynthesis</topic><topic>Animals</topic><topic>arochlor</topic><topic>Aroclors - pharmacology</topic><topic>Aryl Hydrocarbon Hydroxylases - biosynthesis</topic><topic>benzo(a)pyrene</topic><topic>Biological and medical sciences</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Chlorodiphenyl (54% Chlorine)</topic><topic>cytochrome P-450</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>cytochromes</topic><topic>Enzyme Induction</topic><topic>Freezing</topic><topic>frozen storage</topic><topic>genotoxicity testing</topic><topic>In Vitro Techniques</topic><topic>liver</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>mitochondria</topic><topic>Mutagenicity Tests</topic><topic>Mutagens</topic><topic>Oxygenases - biosynthesis</topic><topic>Polychlorinated Biphenyls - pharmacology</topic><topic>Preservation, Biological</topic><topic>Rats</topic><topic>S-9</topic><topic>Salmonella typhimurium - genetics</topic><topic>Specimen Handling</topic><topic>stability</topic><topic>Subcellular Fractions - metabolism</topic><topic>Toxicology</topic><topic>Various organic compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oldham, J.W</creatorcontrib><creatorcontrib>Pritchard, J.F</creatorcontrib><creatorcontrib>Preston, R.F</creatorcontrib><creatorcontrib>Nomides, C.T</creatorcontrib><creatorcontrib>Patton, W.E</creatorcontrib><creatorcontrib>Paulson, J.D</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of applied toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oldham, J.W</au><au>Pritchard, J.F</au><au>Preston, R.F</au><au>Nomides, C.T</au><au>Patton, W.E</au><au>Paulson, J.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability of Aroclor 1254-induced rat liver postmitochondrial supernatant (S-9) following long-term storage (-75 degrees C)</atitle><jtitle>Journal of applied toxicology</jtitle><addtitle>J. Appl. Toxicol</addtitle><date>1985-06</date><risdate>1985</risdate><volume>5</volume><issue>3</issue><spage>187</spage><epage>191</epage><pages>187-191</pages><issn>0260-437X</issn><eissn>1099-1263</eissn><coden>JJATDK</coden><abstract>The stability of Aroclor 1254‐induced rat liver postmitochondrial supernatant (S‐9) over a 5‐year period was investigated in a retrospective study. S‐9 was uniformly prepared at 6‐month intervals, and aliquots were stored at −75°C. The protein and cytochrome P‐450 content of these lots of S‐9 were very similar, and no differences attributable to duration of storage were observed in the activities of ethoxycoumarin O‐deethylase, aniline hydroxyiase, cytochrome P‐450 reductase or aryl hydrocarbon hydroxylase. There was no decrease following 5 years of storage in the ability of S‐9 to activate 2‐aminoanthracene, as measured in the Ames test (TA98), but there was a notable reduction following more than 1 year of storage in the ability of the S‐9 to generate Ames test activity with benzo(a)pyrene. Based on the results of these studies, S‐9 prepared and stored under these conditions appears to be suitable for use in in vitro genotoxicity assays for at least 1 year.</abstract><cop>Chichester</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>3924988</pmid><doi>10.1002/jat.2550050310</doi><tpages>5</tpages></addata></record>
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subjects 2-aminoanthracene
7-Alkoxycoumarin O-Dealkylase
activating system
Ames test
Aniline Hydroxylase - biosynthesis
Animals
arochlor
Aroclors - pharmacology
Aryl Hydrocarbon Hydroxylases - biosynthesis
benzo(a)pyrene
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chlorodiphenyl (54% Chlorine)
cytochrome P-450
Cytochrome P-450 Enzyme System - biosynthesis
cytochromes
Enzyme Induction
Freezing
frozen storage
genotoxicity testing
In Vitro Techniques
liver
Liver - metabolism
Male
Medical sciences
mitochondria
Mutagenicity Tests
Mutagens
Oxygenases - biosynthesis
Polychlorinated Biphenyls - pharmacology
Preservation, Biological
Rats
S-9
Salmonella typhimurium - genetics
Specimen Handling
stability
Subcellular Fractions - metabolism
Toxicology
Various organic compounds
title Stability of Aroclor 1254-induced rat liver postmitochondrial supernatant (S-9) following long-term storage (-75 degrees C)
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