Stimulation of bone healing by transforming growth factor-beta 1 released from polymeric or ceramic implants
The ability to transforming growth factor-beta 1 (TGF-beta 1), to stimulate bone healing was evaluated in a rat critical calvarial defect model. Both a low dose and a high dose of TGF-beta 1 were incorporated into two different types of implants: one made from a composite of poly(lactic-co-glycolic...
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| Veröffentlicht in: | Journal of applied biomaterials 1994-06, Vol.5 (2), p.141-150 |
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| creator | Gombotz, W R Pankey, S C Bouchard, L S Phan, D H Puolakkainen, P A |
| description | The ability to transforming growth factor-beta 1 (TGF-beta 1), to stimulate bone healing was evaluated in a rat critical calvarial defect model. Both a low dose and a high dose of TGF-beta 1 were incorporated into two different types of implants: one made from a composite of poly(lactic-co-glycolic acid) (PLPG) (50:50) and demineralized bone matrix (DBM), and the other from calcium sulfate (CaSO 4). Scanning electron microscopy showed that the CaSO 4 implants were more porous than the PLPG/DBM samples. Both types of implants released biologically active TGF-beta 1 for over 300 h in vitro. The samples were implanted in a 9-mm diameter rat calvarial defect for 6 weeks along with contralateral control implants containing no TGF-beta 1. Microradiography and histological analysis were used to assess the bone healing in the defects. Microradiography revealed that the greatest amount of calcified bone (67.5%) was present in in the CaSO 4 implants containing a high dose of TGF-beta 1 while minimal new bone formation occurred in the PLPG/DBM implants. Histologically, the PLPG/DBM implants exhibited an inflammatory response with little mineralization or bone formation. The defects containing the PLPG/DBM implants consisted of a connective tissue stroma with large void spaces. Giant cells and numerous polymorphonuclear leukocytes were present throughout the implants. In contrast, the CaSO 4 implants had only a few inflammatory cells and the presence of mineralization and true bone was a more consistent feature. These preliminary studies show that TGF-beta 1 is capable of inducing new bone formation. Furthermore, the materials used to deliver the growth factor can play a significant role in the bone healing process. |
| doi_str_mv | 10.1002/jab.770050207 |
| format | Article |
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Both a low dose and a high dose of TGF-beta 1 were incorporated into two different types of implants: one made from a composite of poly(lactic-co-glycolic acid) (PLPG) (50:50) and demineralized bone matrix (DBM), and the other from calcium sulfate (CaSO 4). Scanning electron microscopy showed that the CaSO 4 implants were more porous than the PLPG/DBM samples. Both types of implants released biologically active TGF-beta 1 for over 300 h in vitro. The samples were implanted in a 9-mm diameter rat calvarial defect for 6 weeks along with contralateral control implants containing no TGF-beta 1. Microradiography and histological analysis were used to assess the bone healing in the defects. Microradiography revealed that the greatest amount of calcified bone (67.5%) was present in in the CaSO 4 implants containing a high dose of TGF-beta 1 while minimal new bone formation occurred in the PLPG/DBM implants. Histologically, the PLPG/DBM implants exhibited an inflammatory response with little mineralization or bone formation. The defects containing the PLPG/DBM implants consisted of a connective tissue stroma with large void spaces. Giant cells and numerous polymorphonuclear leukocytes were present throughout the implants. In contrast, the CaSO 4 implants had only a few inflammatory cells and the presence of mineralization and true bone was a more consistent feature. These preliminary studies show that TGF-beta 1 is capable of inducing new bone formation. Furthermore, the materials used to deliver the growth factor can play a significant role in the bone healing process.</description><identifier>ISSN: 1045-4861</identifier><identifier>EISSN: 1549-9316</identifier><identifier>DOI: 10.1002/jab.770050207</identifier><identifier>PMID: 10172073</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Biocompatible Materials ; Biodegradation, Environmental ; Bone Regeneration - drug effects ; Bone Regeneration - physiology ; Calcium Sulfate - chemistry ; Calcium Sulfate - pharmacokinetics ; Growth Substances - administration & dosage ; Growth Substances - chemistry ; Growth Substances - pharmacokinetics ; Materials Testing ; Microscopy, Electron, Scanning ; Polymers - chemistry ; Polymers - pharmacokinetics ; Porosity ; Prostheses and Implants ; Rabbits ; Rats, Sprague-Dawley ; Wound Healing</subject><ispartof>Journal of applied biomaterials, 1994-06, Vol.5 (2), p.141-150</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c221t-427bf8625ab28ac89606b07d9f42a9bd3b9c9e6ac96c1372f7a8e2a4c6a743243</citedby><cites>FETCH-LOGICAL-c221t-427bf8625ab28ac89606b07d9f42a9bd3b9c9e6ac96c1372f7a8e2a4c6a743243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10172073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gombotz, W R</creatorcontrib><creatorcontrib>Pankey, S C</creatorcontrib><creatorcontrib>Bouchard, L S</creatorcontrib><creatorcontrib>Phan, D H</creatorcontrib><creatorcontrib>Puolakkainen, P A</creatorcontrib><title>Stimulation of bone healing by transforming growth factor-beta 1 released from polymeric or ceramic implants</title><title>Journal of applied biomaterials</title><addtitle>J Appl Biomater</addtitle><description>The ability to transforming growth factor-beta 1 (TGF-beta 1), to stimulate bone healing was evaluated in a rat critical calvarial defect model. Both a low dose and a high dose of TGF-beta 1 were incorporated into two different types of implants: one made from a composite of poly(lactic-co-glycolic acid) (PLPG) (50:50) and demineralized bone matrix (DBM), and the other from calcium sulfate (CaSO 4). Scanning electron microscopy showed that the CaSO 4 implants were more porous than the PLPG/DBM samples. Both types of implants released biologically active TGF-beta 1 for over 300 h in vitro. The samples were implanted in a 9-mm diameter rat calvarial defect for 6 weeks along with contralateral control implants containing no TGF-beta 1. Microradiography and histological analysis were used to assess the bone healing in the defects. Microradiography revealed that the greatest amount of calcified bone (67.5%) was present in in the CaSO 4 implants containing a high dose of TGF-beta 1 while minimal new bone formation occurred in the PLPG/DBM implants. Histologically, the PLPG/DBM implants exhibited an inflammatory response with little mineralization or bone formation. The defects containing the PLPG/DBM implants consisted of a connective tissue stroma with large void spaces. Giant cells and numerous polymorphonuclear leukocytes were present throughout the implants. In contrast, the CaSO 4 implants had only a few inflammatory cells and the presence of mineralization and true bone was a more consistent feature. These preliminary studies show that TGF-beta 1 is capable of inducing new bone formation. Furthermore, the materials used to deliver the growth factor can play a significant role in the bone healing process.</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Biodegradation, Environmental</subject><subject>Bone Regeneration - drug effects</subject><subject>Bone Regeneration - physiology</subject><subject>Calcium Sulfate - chemistry</subject><subject>Calcium Sulfate - pharmacokinetics</subject><subject>Growth Substances - administration & dosage</subject><subject>Growth Substances - chemistry</subject><subject>Growth Substances - pharmacokinetics</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Polymers - chemistry</subject><subject>Polymers - pharmacokinetics</subject><subject>Porosity</subject><subject>Prostheses and Implants</subject><subject>Rabbits</subject><subject>Rats, Sprague-Dawley</subject><subject>Wound Healing</subject><issn>1045-4861</issn><issn>1549-9316</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLAzEYRYMotlaXbiV_YGpek0yWUnxBwYW6Hr5kknbKZFKSFOm_d6Qiru69cLiLg9AtJUtKCLvfgVkqRUhNGFFnaE5roSvNqTyfOhF1JRpJZ-gq5x0hnDJRX6IZJVRNOJ-j4b304TBA6eOIo8cmjg5vHQz9uMHmiEuCMfuYws_epPhVttiDLTFVxhXAFCc3OMiuwz7FgPdxOAaXeotjwtYlCFPtw36AseRrdOFhyO7mNxfo8-nxY_VSrd-eX1cP68oyRkslmDK-kawGwxqwjZZEGqI67QUDbTputNVOgtXSUq6YV9A4BsJKUIIzwReoOv3aFHNOzrf71AdIx5aS9sdaO1lr_6xN_N2J3x9McN0_-qSJfwN61mnM</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>Gombotz, W R</creator><creator>Pankey, S C</creator><creator>Bouchard, L S</creator><creator>Phan, D H</creator><creator>Puolakkainen, P A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19940601</creationdate><title>Stimulation of bone healing by transforming growth factor-beta 1 released from polymeric or ceramic implants</title><author>Gombotz, W R ; Pankey, S C ; Bouchard, L S ; Phan, D H ; Puolakkainen, P A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c221t-427bf8625ab28ac89606b07d9f42a9bd3b9c9e6ac96c1372f7a8e2a4c6a743243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Biodegradation, Environmental</topic><topic>Bone Regeneration - drug effects</topic><topic>Bone Regeneration - physiology</topic><topic>Calcium Sulfate - chemistry</topic><topic>Calcium Sulfate - pharmacokinetics</topic><topic>Growth Substances - administration & dosage</topic><topic>Growth Substances - chemistry</topic><topic>Growth Substances - pharmacokinetics</topic><topic>Materials Testing</topic><topic>Microscopy, Electron, Scanning</topic><topic>Polymers - chemistry</topic><topic>Polymers - pharmacokinetics</topic><topic>Porosity</topic><topic>Prostheses and Implants</topic><topic>Rabbits</topic><topic>Rats, Sprague-Dawley</topic><topic>Wound Healing</topic><toplevel>online_resources</toplevel><creatorcontrib>Gombotz, W R</creatorcontrib><creatorcontrib>Pankey, S C</creatorcontrib><creatorcontrib>Bouchard, L S</creatorcontrib><creatorcontrib>Phan, D H</creatorcontrib><creatorcontrib>Puolakkainen, P A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of applied biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gombotz, W R</au><au>Pankey, S C</au><au>Bouchard, L S</au><au>Phan, D H</au><au>Puolakkainen, P A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulation of bone healing by transforming growth factor-beta 1 released from polymeric or ceramic implants</atitle><jtitle>Journal of applied biomaterials</jtitle><addtitle>J Appl Biomater</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>5</volume><issue>2</issue><spage>141</spage><epage>150</epage><pages>141-150</pages><issn>1045-4861</issn><eissn>1549-9316</eissn><abstract>The ability to transforming growth factor-beta 1 (TGF-beta 1), to stimulate bone healing was evaluated in a rat critical calvarial defect model. Both a low dose and a high dose of TGF-beta 1 were incorporated into two different types of implants: one made from a composite of poly(lactic-co-glycolic acid) (PLPG) (50:50) and demineralized bone matrix (DBM), and the other from calcium sulfate (CaSO 4). Scanning electron microscopy showed that the CaSO 4 implants were more porous than the PLPG/DBM samples. Both types of implants released biologically active TGF-beta 1 for over 300 h in vitro. The samples were implanted in a 9-mm diameter rat calvarial defect for 6 weeks along with contralateral control implants containing no TGF-beta 1. Microradiography and histological analysis were used to assess the bone healing in the defects. Microradiography revealed that the greatest amount of calcified bone (67.5%) was present in in the CaSO 4 implants containing a high dose of TGF-beta 1 while minimal new bone formation occurred in the PLPG/DBM implants. Histologically, the PLPG/DBM implants exhibited an inflammatory response with little mineralization or bone formation. The defects containing the PLPG/DBM implants consisted of a connective tissue stroma with large void spaces. Giant cells and numerous polymorphonuclear leukocytes were present throughout the implants. In contrast, the CaSO 4 implants had only a few inflammatory cells and the presence of mineralization and true bone was a more consistent feature. These preliminary studies show that TGF-beta 1 is capable of inducing new bone formation. Furthermore, the materials used to deliver the growth factor can play a significant role in the bone healing process.</abstract><cop>United States</cop><pmid>10172073</pmid><doi>10.1002/jab.770050207</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biocompatible Materials Biodegradation, Environmental Bone Regeneration - drug effects Bone Regeneration - physiology Calcium Sulfate - chemistry Calcium Sulfate - pharmacokinetics Growth Substances - administration & dosage Growth Substances - chemistry Growth Substances - pharmacokinetics Materials Testing Microscopy, Electron, Scanning Polymers - chemistry Polymers - pharmacokinetics Porosity Prostheses and Implants Rabbits Rats, Sprague-Dawley Wound Healing |
| title | Stimulation of bone healing by transforming growth factor-beta 1 released from polymeric or ceramic implants |
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