Methylation of DNA in target and non‐target organs of the rat with methylbenzylnitrosamine and dimethylnitrosamine

The sites of labelling of DNA with [14C]methyl groups from methylbenzylnitrosamine and dimethylnitrosamine were studied in rat oeso‐phageal epithelium and liver. All four combinations of tissue and carcinogen were studied. Tissues were labelled in vitro and the DNA contained therein purified and hyd...

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Veröffentlicht in:	International journal of cancer 1979-05, Vol.23 (5), p.679-682
Hauptverfasser:	Fong, Louise Y. Y., Lin, Hsiang Ju, Lee, Clara Lai Hing
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Thin Layer Dimethylnitrosamine - metabolism DNA - analysis DNA - isolation & purification DNA - metabolism Epithelium - metabolism Esophagus - metabolism Hydrolysis Liver - metabolism Male Methylation Nitrosamines - metabolism Rats
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container_end_page	682
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container_title	International journal of cancer
container_volume	23
creator	Fong, Louise Y. Y. Lin, Hsiang Ju Lee, Clara Lai Hing
description	The sites of labelling of DNA with [14C]methyl groups from methylbenzylnitrosamine and dimethylnitrosamine were studied in rat oeso‐phageal epithelium and liver. All four combinations of tissue and carcinogen were studied. Tissues were labelled in vitro and the DNA contained therein purified and hydrolysed (pH 1, 37° C) to free purines and apurinic acid. Quantitative analysis was performed with the aid of thin‐layer chromatography. The apurinic acid and 7‐methylguanine fractions were found to be extensively labelled. Smaller amounts of radioactivity were found in O6‐methyl‐guanine and some of the methylated adenines. The same carcinogen produced different patterns of labelling in oesophageal and liver DNA. The proportion of O6‐methylguanine to 7‐methylguanine was higher when the methylating agent was a carcinogen specific for the organ.
doi_str_mv	10.1002/ijc.2910230514
format	Article
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The proportion of O6‐methylguanine to 7‐methylguanine was higher when the methylating agent was a carcinogen specific for the organ.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910230514</identifier><identifier>PMID: 457309</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Chromatography, Thin Layer ; Dimethylnitrosamine - metabolism ; DNA - analysis ; DNA - isolation & purification ; DNA - metabolism ; Epithelium - metabolism ; Esophagus - metabolism ; Hydrolysis ; Liver - metabolism ; Male ; Methylation ; Nitrosamines - metabolism ; Rats</subject><ispartof>International journal of cancer, 1979-05, Vol.23 (5), p.679-682</ispartof><rights>Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3394-869105d55bbd3fc8aab1693f0acb519811b32ab5c96668b9469a982acc42ad343</citedby><cites>FETCH-LOGICAL-c3394-869105d55bbd3fc8aab1693f0acb519811b32ab5c96668b9469a982acc42ad343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910230514$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910230514$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/457309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fong, Louise Y. Y.</creatorcontrib><creatorcontrib>Lin, Hsiang Ju</creatorcontrib><creatorcontrib>Lee, Clara Lai Hing</creatorcontrib><title>Methylation of DNA in target and non‐target organs of the rat with methylbenzylnitrosamine and dimethylnitrosamine</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>The sites of labelling of DNA with [14C]methyl groups from methylbenzylnitrosamine and dimethylnitrosamine were studied in rat oeso‐phageal epithelium and liver. All four combinations of tissue and carcinogen were studied. Tissues were labelled in vitro and the DNA contained therein purified and hydrolysed (pH 1, 37° C) to free purines and apurinic acid. Quantitative analysis was performed with the aid of thin‐layer chromatography. The apurinic acid and 7‐methylguanine fractions were found to be extensively labelled. Smaller amounts of radioactivity were found in O6‐methyl‐guanine and some of the methylated adenines. The same carcinogen produced different patterns of labelling in oesophageal and liver DNA. The proportion of O6‐methylguanine to 7‐methylguanine was higher when the methylating agent was a carcinogen specific for the organ.</description><subject>Animals</subject><subject>Chromatography, Thin Layer</subject><subject>Dimethylnitrosamine - metabolism</subject><subject>DNA - analysis</subject><subject>DNA - isolation & purification</subject><subject>DNA - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Esophagus - metabolism</subject><subject>Hydrolysis</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Methylation</subject><subject>Nitrosamines - metabolism</subject><subject>Rats</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1OwzAQhS3EXyhsWbHwBVLsOHbjZVX-igpsYB2NHad1lThVYlSFFUfgjJyEtKmgO1YjzXvvG81D6JKSISUkurZLPYwkJREjnMYHKKBEjkISUX6Igs5AwhFl4hSdNc2SEEo5iU_QccxHjMgA-SfjF20B3lYOVzm-eR5j67CHem48BpdhV7nvz6_doqrn4JqN0S8MrsHjtfULXG4hyriPtnDW11UDpXVmm89sr-7tz9FRDkVjLnZzgN7ubl8nD-Hs5X46Gc9CzZiMw0R0b_GMc6UylusEQFEhWU5AK05lQqliESiupRAiUTIWEmQSgdZxBBmL2QANe67uLje1ydNVbUuo25SSdFNe2pWX_pXXBa76wOpdlSb7tfdtdbLs5bUtTPsPLJ0-TvbQP2HWfik</recordid><startdate>19790515</startdate><enddate>19790515</enddate><creator>Fong, Louise Y. 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Y. ; Lin, Hsiang Ju ; Lee, Clara Lai Hing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3394-869105d55bbd3fc8aab1693f0acb519811b32ab5c96668b9469a982acc42ad343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Chromatography, Thin Layer</topic><topic>Dimethylnitrosamine - metabolism</topic><topic>DNA - analysis</topic><topic>DNA - isolation & purification</topic><topic>DNA - metabolism</topic><topic>Epithelium - metabolism</topic><topic>Esophagus - metabolism</topic><topic>Hydrolysis</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Methylation</topic><topic>Nitrosamines - metabolism</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fong, Louise Y. Y.</creatorcontrib><creatorcontrib>Lin, Hsiang Ju</creatorcontrib><creatorcontrib>Lee, Clara Lai Hing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fong, Louise Y. Y.</au><au>Lin, Hsiang Ju</au><au>Lee, Clara Lai Hing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylation of DNA in target and non‐target organs of the rat with methylbenzylnitrosamine and dimethylnitrosamine</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1979-05-15</date><risdate>1979</risdate><volume>23</volume><issue>5</issue><spage>679</spage><epage>682</epage><pages>679-682</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><abstract>The sites of labelling of DNA with [14C]methyl groups from methylbenzylnitrosamine and dimethylnitrosamine were studied in rat oeso‐phageal epithelium and liver. All four combinations of tissue and carcinogen were studied. Tissues were labelled in vitro and the DNA contained therein purified and hydrolysed (pH 1, 37° C) to free purines and apurinic acid. Quantitative analysis was performed with the aid of thin‐layer chromatography. The apurinic acid and 7‐methylguanine fractions were found to be extensively labelled. Smaller amounts of radioactivity were found in O6‐methyl‐guanine and some of the methylated adenines. The same carcinogen produced different patterns of labelling in oesophageal and liver DNA. The proportion of O6‐methylguanine to 7‐methylguanine was higher when the methylating agent was a carcinogen specific for the organ.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>457309</pmid><doi>10.1002/ijc.2910230514</doi><tpages>4</tpages></addata></record>
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subjects	Animals Chromatography, Thin Layer Dimethylnitrosamine - metabolism DNA - analysis DNA - isolation & purification DNA - metabolism Epithelium - metabolism Esophagus - metabolism Hydrolysis Liver - metabolism Male Methylation Nitrosamines - metabolism Rats
title	Methylation of DNA in target and non‐target organs of the rat with methylbenzylnitrosamine and dimethylnitrosamine
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