Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation
The steady‐state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)‐induced cell killing. A block in G2/M phase, larger peak of apoptotic cells and higher caspase‐3 in vitro activity were noted in nucleo...
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| Veröffentlicht in: | International journal of cancer 2002-01, Vol.97 (3), p.297-305 |
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| container_title | International journal of cancer |
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| creator | Wu, Ming H. Chang, Jei H. Chou, Chih C. Yung, Benjamin Y.M. |
| description | The steady‐state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)‐induced cell killing. A block in G2/M phase, larger peak of apoptotic cells and higher caspase‐3 in vitro activity were noted in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells after UV treatment. Irradiated cells that received vector plasmid exhibited increased levels of [3H]thymidine incorporation due to DNA repair synthesis. In contrast, irradiated cells that received nucleophosmin/B23 antisense plasmid exhibited no such increase of [3H]thymidine incorporation, indicating inhibition of DNA repair. Cotransfection of cells with vector allowed repair of the damaged chloramphenicol acetyl transferase (CAT) reporter and rescue of CAT activity by host repair machinery. CAT activity in cells cotransfected with nucleophosmin/B23 antisense was less ( |
| doi_str_mv | 10.1002/ijc.1606 |
| format | Article |
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Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)‐induced cell killing. A block in G2/M phase, larger peak of apoptotic cells and higher caspase‐3 in vitro activity were noted in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells after UV treatment. Irradiated cells that received vector plasmid exhibited increased levels of [3H]thymidine incorporation due to DNA repair synthesis. In contrast, irradiated cells that received nucleophosmin/B23 antisense plasmid exhibited no such increase of [3H]thymidine incorporation, indicating inhibition of DNA repair. Cotransfection of cells with vector allowed repair of the damaged chloramphenicol acetyl transferase (CAT) reporter and rescue of CAT activity by host repair machinery. CAT activity in cells cotransfected with nucleophosmin/B23 antisense was less (<50%) than that of vector‐transfected cells, indicating reduction of host nucleotide excision repair activity. Lower protein expressions of nucleophosmin/B23 and proliferating cell nuclear antigen (PCNA) were observed in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells with or without UV treatment. Cotransfection of nucleophosmin/B23 antisense‐transfected HeLa cells with PCNA construct made the cells less susceptible to UV‐induced cell killing. Our results indicate that nucleophosmin/B23 correlates with PCNA and DNA repair capacity in cellular sensitivity to UV. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.1606</identifier><identifier>PMID: 11774280</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Biological and medical sciences ; Biological effects of radiation ; Caspase 3 ; Caspases - metabolism ; Chloramphenicol O-Acetyltransferase - metabolism ; DNA - radiation effects ; DNA Repair ; Dose-Response Relationship, Radiation ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Genes, Reporter ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; HeLa Cells ; Humans ; Immunoblotting ; Luciferases - metabolism ; Non ionizing radiations. Hertzian waves. Biooptics ; Nuclear Proteins - metabolism ; nucleophosmin/B23 ; Oligonucleotides, Antisense - pharmacology ; PCNA ; Plasmids - metabolism ; Proliferating Cell Nuclear Antigen - metabolism ; RNA - metabolism ; RNA, Messenger - metabolism ; Time Factors ; Tissues, organs and organisms biophysics ; Transfection ; Ultraviolet Rays</subject><ispartof>International journal of cancer, 2002-01, Vol.97 (3), p.297-305</ispartof><rights>Copyright © 2002 Wiley‐Liss, Inc.</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3626-598ce70267ee448e5ac0dcb67ea26a8ee5c819926fe2701f77e007d3027bfa13</citedby><cites>FETCH-LOGICAL-c3626-598ce70267ee448e5ac0dcb67ea26a8ee5c819926fe2701f77e007d3027bfa13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.1606$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.1606$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13447141$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11774280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Ming H.</creatorcontrib><creatorcontrib>Chang, Jei H.</creatorcontrib><creatorcontrib>Chou, Chih C.</creatorcontrib><creatorcontrib>Yung, Benjamin Y.M.</creatorcontrib><title>Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>The steady‐state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)‐induced cell killing. A block in G2/M phase, larger peak of apoptotic cells and higher caspase‐3 in vitro activity were noted in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells after UV treatment. Irradiated cells that received vector plasmid exhibited increased levels of [3H]thymidine incorporation due to DNA repair synthesis. In contrast, irradiated cells that received nucleophosmin/B23 antisense plasmid exhibited no such increase of [3H]thymidine incorporation, indicating inhibition of DNA repair. Cotransfection of cells with vector allowed repair of the damaged chloramphenicol acetyl transferase (CAT) reporter and rescue of CAT activity by host repair machinery. CAT activity in cells cotransfected with nucleophosmin/B23 antisense was less (<50%) than that of vector‐transfected cells, indicating reduction of host nucleotide excision repair activity. Lower protein expressions of nucleophosmin/B23 and proliferating cell nuclear antigen (PCNA) were observed in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells with or without UV treatment. Cotransfection of nucleophosmin/B23 antisense‐transfected HeLa cells with PCNA construct made the cells less susceptible to UV‐induced cell killing. Our results indicate that nucleophosmin/B23 correlates with PCNA and DNA repair capacity in cellular sensitivity to UV. © 2001 Wiley‐Liss, Inc.</description><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>DNA - radiation effects</subject><subject>DNA Repair</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Reporter</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Luciferases - metabolism</subject><subject>Non ionizing radiations. Hertzian waves. Biooptics</subject><subject>Nuclear Proteins - metabolism</subject><subject>nucleophosmin/B23</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>PCNA</subject><subject>Plasmids - metabolism</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>RNA - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Transfection</subject><subject>Ultraviolet Rays</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10EFLwzAUB_AgiptT8BNILoKXbi9pm3RHHeomAz1MryVLX1lGm5SkTvbtbd1gJ0-Px_vx_vAn5JbBmAHwidnqMRMgzsiQwVRGwFl6TobdCSLJYjEgVyFsARhLIbkkA8akTHgGQ_KxsDtX7bBG21JXUvutK3TNxoXa2MkTj6mxtN0g9RgaZwP2aI5LRTVWVaCto59f1HivCqNa4-w1uShVFfDmOEdk9fK8ms2j5fvrYva4jHQsuIjSaaZRAhcSMUkyTJWGQq-7VXGhMsRUZ2w65aJELoGVUiKALGLgcl0qFo_Iw-Gt9i4Ej2XeeFMrv88Z5H0neddJ3nfS0bsDbb7XNRYneCyhA_dHoIJWVemV1SacXJwkkiV9ZnRwP6bC_b-B-eJt9hf8C1UOdpw</recordid><startdate>20020120</startdate><enddate>20020120</enddate><creator>Wu, Ming H.</creator><creator>Chang, Jei H.</creator><creator>Chou, Chih C.</creator><creator>Yung, Benjamin Y.M.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020120</creationdate><title>Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation</title><author>Wu, Ming H. ; Chang, Jei H. ; Chou, Chih C. ; Yung, Benjamin Y.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3626-598ce70267ee448e5ac0dcb67ea26a8ee5c819926fe2701f77e007d3027bfa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>DNA - radiation effects</topic><topic>DNA Repair</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Reporter</topic><topic>Genetic Vectors - genetics</topic><topic>Genetic Vectors - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Luciferases - metabolism</topic><topic>Non ionizing radiations. Hertzian waves. Biooptics</topic><topic>Nuclear Proteins - metabolism</topic><topic>nucleophosmin/B23</topic><topic>Oligonucleotides, Antisense - pharmacology</topic><topic>PCNA</topic><topic>Plasmids - metabolism</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>RNA - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Transfection</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Ming H.</creatorcontrib><creatorcontrib>Chang, Jei H.</creatorcontrib><creatorcontrib>Chou, Chih C.</creatorcontrib><creatorcontrib>Yung, Benjamin Y.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Ming H.</au><au>Chang, Jei H.</au><au>Chou, Chih C.</au><au>Yung, Benjamin Y.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>2002-01-20</date><risdate>2002</risdate><volume>97</volume><issue>3</issue><spage>297</spage><epage>305</epage><pages>297-305</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>The steady‐state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)‐induced cell killing. A block in G2/M phase, larger peak of apoptotic cells and higher caspase‐3 in vitro activity were noted in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells after UV treatment. Irradiated cells that received vector plasmid exhibited increased levels of [3H]thymidine incorporation due to DNA repair synthesis. In contrast, irradiated cells that received nucleophosmin/B23 antisense plasmid exhibited no such increase of [3H]thymidine incorporation, indicating inhibition of DNA repair. Cotransfection of cells with vector allowed repair of the damaged chloramphenicol acetyl transferase (CAT) reporter and rescue of CAT activity by host repair machinery. CAT activity in cells cotransfected with nucleophosmin/B23 antisense was less (<50%) than that of vector‐transfected cells, indicating reduction of host nucleotide excision repair activity. Lower protein expressions of nucleophosmin/B23 and proliferating cell nuclear antigen (PCNA) were observed in nucleophosmin/B23 antisense‐transfected cells compared with vector‐transfected cells with or without UV treatment. Cotransfection of nucleophosmin/B23 antisense‐transfected HeLa cells with PCNA construct made the cells less susceptible to UV‐induced cell killing. Our results indicate that nucleophosmin/B23 correlates with PCNA and DNA repair capacity in cellular sensitivity to UV. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11774280</pmid><doi>10.1002/ijc.1606</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Biological effects of radiation Caspase 3 Caspases - metabolism Chloramphenicol O-Acetyltransferase - metabolism DNA - radiation effects DNA Repair Dose-Response Relationship, Radiation Flow Cytometry Fundamental and applied biological sciences. Psychology Genes, Reporter Genetic Vectors - genetics Genetic Vectors - metabolism HeLa Cells Humans Immunoblotting Luciferases - metabolism Non ionizing radiations. Hertzian waves. Biooptics Nuclear Proteins - metabolism nucleophosmin/B23 Oligonucleotides, Antisense - pharmacology PCNA Plasmids - metabolism Proliferating Cell Nuclear Antigen - metabolism RNA - metabolism RNA, Messenger - metabolism Time Factors Tissues, organs and organisms biophysics Transfection Ultraviolet Rays |
| title | Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation |
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