A colorimetric assay of lysogenic induction designed for screening potential carcinogenic and carcinostatic agents

Simple, rapid colorimetric tests for lysogenic induction (the derepression of a latent bacterial virus) are described. A quantitative test and a more rapid semiquantitative test are based on the assay of the β‐galactosidase synthesized from a lacZ gene fused to an operon under lambda repressor contr...

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Veröffentlicht in:	Environmental mutagenesis 1979, Vol.1 (1), p.65-78
Hauptverfasser:	Elespuru, R. K., Yarmolinsky, M. B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - genetics cancer chemotherapy Carcinogens - pharmacology Colorimetry - methods DNA - genetics DNA damage Drug Evaluation, Preclinical - methods Escherichia coli - genetics Genetic Techniques inductest Mutagens SOS response Virus Activation - drug effects
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Zusammenfassung:	Simple, rapid colorimetric tests for lysogenic induction (the derepression of a latent bacterial virus) are described. A quantitative test and a more rapid semiquantitative test are based on the assay of the β‐galactosidase synthesized from a lacZ gene fused to an operon under lambda repressor control. These biochemical “inductests” are suitable for screening programs designed to detect agents that damage DNA and that are of potential interest in carcinogenesis and cancer chemotherapy.
ISSN:	0192-2521 1930-238X
DOI:	10.1002/em.2860010113