N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots
The association of immunoglobulin G (IgG) glycosylation changes with various human diseases and physiological conditions is well established. Since the mechanistical explanation of the regulation of IgG glycosylation and its functional role in these various states is still missing, the eyes of the b...
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| Veröffentlicht in: | Electrophoresis 2021-12, Vol.42 (24), p.2615-2618 |
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| creator | Patenaude, Anne‐Marie Erhardt, Julija Hennig, René Rapp, Erdmann Lauc, Gordan Pezer, Marija |
| description | The association of immunoglobulin G (IgG) glycosylation changes with various human diseases and physiological conditions is well established. Since the mechanistical explanation of the regulation of IgG glycosylation and its functional role in these various states is still missing, the eyes of the biomedical community are now turned towards animal models, which enable intervention studies necessary for conclusions on causality. Mice are recognized and used as a good experimental model for human IgG glycosylation. However, smaller blood volumes, low IgG concentrations at young ages (which are most often used in mice experiments) and multiple sampling protocols during the course of longitudinal studies would profit from a robust workflow for mouse IgG glycome analysis from minute amounts of starting material, collected through a simple sampling procedure. For this purpose, we have developed a protocol for analysis of total N‐glycans of IgG isolated from mouse dried blood spots (DBS), which we report here. We show that mouse DBS are a good source of material for IgG N‐glycan analysis by multiplexed capillary gel electrophoresis with laser‐induced fluorescence (xCGE‐LIF). |
| doi_str_mv | 10.1002/elps.202000249 |
| format | Article |
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Since the mechanistical explanation of the regulation of IgG glycosylation and its functional role in these various states is still missing, the eyes of the biomedical community are now turned towards animal models, which enable intervention studies necessary for conclusions on causality. Mice are recognized and used as a good experimental model for human IgG glycosylation. However, smaller blood volumes, low IgG concentrations at young ages (which are most often used in mice experiments) and multiple sampling protocols during the course of longitudinal studies would profit from a robust workflow for mouse IgG glycome analysis from minute amounts of starting material, collected through a simple sampling procedure. For this purpose, we have developed a protocol for analysis of total N‐glycans of IgG isolated from mouse dried blood spots (DBS), which we report here. We show that mouse DBS are a good source of material for IgG N‐glycan analysis by multiplexed capillary gel electrophoresis with laser‐induced fluorescence (xCGE‐LIF).</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.202000249</identifier><identifier>PMID: 33165939</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Animals ; Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biomarker ; Biomedical materials ; Blood ; Capillary gel electrophoresis laser‐induced fluorescence ; Chemistry ; Chemistry, Analytical ; Dried Blood Spot Testing ; Dried blood spots ; Electrophoresis ; Electrophoresis, Capillary ; Fluorescence ; Glycan ; Glycosylation ; IgG antibody ; IgG glycome ; Immunoglobulin G - blood ; Immunoglobulins ; Life Sciences & Biomedicine ; Mice ; Mouse ; Physical Sciences ; Polysaccharides - chemistry ; Sampling ; Science & Technology ; Workflow</subject><ispartof>Electrophoresis, 2021-12, Vol.42 (24), p.2615-2618</ispartof><rights>2020 Wiley‐VCH GmbH</rights><rights>2020 Wiley-VCH GmbH.</rights><rights>2021 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>3</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000591271400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3684-725cd59f8f31cc74a7ec96c65a98e1bd539db6de5f4029f304eca22ef3879efa3</citedby><cites>FETCH-LOGICAL-c3684-725cd59f8f31cc74a7ec96c65a98e1bd539db6de5f4029f304eca22ef3879efa3</cites><orcidid>0000-0002-6480-1427 ; 0009-0000-4748-3220 ; 0000-0001-6618-2626 ; 0000-0003-1840-9560</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.202000249$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.202000249$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,39263,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33165939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Patenaude, Anne‐Marie</creatorcontrib><creatorcontrib>Erhardt, Julija</creatorcontrib><creatorcontrib>Hennig, René</creatorcontrib><creatorcontrib>Rapp, Erdmann</creatorcontrib><creatorcontrib>Lauc, Gordan</creatorcontrib><creatorcontrib>Pezer, Marija</creatorcontrib><title>N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><addtitle>Electrophoresis</addtitle><description>The association of immunoglobulin G (IgG) glycosylation changes with various human diseases and physiological conditions is well established. Since the mechanistical explanation of the regulation of IgG glycosylation and its functional role in these various states is still missing, the eyes of the biomedical community are now turned towards animal models, which enable intervention studies necessary for conclusions on causality. Mice are recognized and used as a good experimental model for human IgG glycosylation. However, smaller blood volumes, low IgG concentrations at young ages (which are most often used in mice experiments) and multiple sampling protocols during the course of longitudinal studies would profit from a robust workflow for mouse IgG glycome analysis from minute amounts of starting material, collected through a simple sampling procedure. For this purpose, we have developed a protocol for analysis of total N‐glycans of IgG isolated from mouse dried blood spots (DBS), which we report here. We show that mouse DBS are a good source of material for IgG N‐glycan analysis by multiplexed capillary gel electrophoresis with laser‐induced fluorescence (xCGE‐LIF).</description><subject>Animals</subject><subject>Biochemical Research Methods</subject><subject>Biochemistry & Molecular Biology</subject><subject>Biomarker</subject><subject>Biomedical materials</subject><subject>Blood</subject><subject>Capillary gel electrophoresis laser‐induced fluorescence</subject><subject>Chemistry</subject><subject>Chemistry, Analytical</subject><subject>Dried Blood Spot Testing</subject><subject>Dried blood spots</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Capillary</subject><subject>Fluorescence</subject><subject>Glycan</subject><subject>Glycosylation</subject><subject>IgG antibody</subject><subject>IgG glycome</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulins</subject><subject>Life Sciences & Biomedicine</subject><subject>Mice</subject><subject>Mouse</subject><subject>Physical Sciences</subject><subject>Polysaccharides - chemistry</subject><subject>Sampling</subject><subject>Science & Technology</subject><subject>Workflow</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqN0c9rFDEUB_Agit1Wrx4l4EWQWfN7kqMstRWWKljPQybzUlIyk3UyQ5mbf4J_o3-JWXfdg5d6ygt83uPlG4ReUbKmhLD3EHd5zQgj5SLME7SikrGKKc2fohWhNa-I5vIMned8X4wwQjxHZ5xTJQ03K3R78-vHz7u4uJSXaKeQBmwHG5ccMk4e92nOgEPfz0O6i6mdYxjwFQ45FQwd9mPqcTeGUrYxpQ7nXZryC_TM25jh5fG8QN8-Xt5urqvt56tPmw_bynGlRVUz6TppvPacOlcLW4MzyilpjQbadpKbrlUdSC8IM54TAc4yBp7r2oC3_AK9Pczdjen7DHlq-pAdxGgHKIs3TEhtlJBcFvrmH3qf5rG8tChFtGbK6L1aH5QbU84j-GY3ht6OS0NJs8-72efdnPIuDa-PY-e2h-7E_wZcwLsDeIA2-ewCDA5OrEyRhrKailIRWrT-f70J058P26R5mEqrOLaGCMsjezeX2y9f6xIo_w3iMK3x</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Patenaude, Anne‐Marie</creator><creator>Erhardt, Julija</creator><creator>Hennig, René</creator><creator>Rapp, Erdmann</creator><creator>Lauc, Gordan</creator><creator>Pezer, Marija</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6480-1427</orcidid><orcidid>https://orcid.org/0009-0000-4748-3220</orcidid><orcidid>https://orcid.org/0000-0001-6618-2626</orcidid><orcidid>https://orcid.org/0000-0003-1840-9560</orcidid></search><sort><creationdate>202112</creationdate><title>N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots</title><author>Patenaude, Anne‐Marie ; Erhardt, Julija ; Hennig, René ; Rapp, Erdmann ; Lauc, Gordan ; Pezer, Marija</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3684-725cd59f8f31cc74a7ec96c65a98e1bd539db6de5f4029f304eca22ef3879efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biochemical Research Methods</topic><topic>Biochemistry & Molecular Biology</topic><topic>Biomarker</topic><topic>Biomedical materials</topic><topic>Blood</topic><topic>Capillary gel electrophoresis laser‐induced fluorescence</topic><topic>Chemistry</topic><topic>Chemistry, Analytical</topic><topic>Dried Blood Spot Testing</topic><topic>Dried blood spots</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Capillary</topic><topic>Fluorescence</topic><topic>Glycan</topic><topic>Glycosylation</topic><topic>IgG antibody</topic><topic>IgG glycome</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulins</topic><topic>Life Sciences & Biomedicine</topic><topic>Mice</topic><topic>Mouse</topic><topic>Physical Sciences</topic><topic>Polysaccharides - chemistry</topic><topic>Sampling</topic><topic>Science & Technology</topic><topic>Workflow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Patenaude, Anne‐Marie</creatorcontrib><creatorcontrib>Erhardt, Julija</creatorcontrib><creatorcontrib>Hennig, René</creatorcontrib><creatorcontrib>Rapp, Erdmann</creatorcontrib><creatorcontrib>Lauc, Gordan</creatorcontrib><creatorcontrib>Pezer, Marija</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Patenaude, Anne‐Marie</au><au>Erhardt, Julija</au><au>Hennig, René</au><au>Rapp, Erdmann</au><au>Lauc, Gordan</au><au>Pezer, Marija</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots</atitle><jtitle>Electrophoresis</jtitle><stitle>ELECTROPHORESIS</stitle><addtitle>Electrophoresis</addtitle><date>2021-12</date><risdate>2021</risdate><volume>42</volume><issue>24</issue><spage>2615</spage><epage>2618</epage><pages>2615-2618</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>The association of immunoglobulin G (IgG) glycosylation changes with various human diseases and physiological conditions is well established. Since the mechanistical explanation of the regulation of IgG glycosylation and its functional role in these various states is still missing, the eyes of the biomedical community are now turned towards animal models, which enable intervention studies necessary for conclusions on causality. Mice are recognized and used as a good experimental model for human IgG glycosylation. However, smaller blood volumes, low IgG concentrations at young ages (which are most often used in mice experiments) and multiple sampling protocols during the course of longitudinal studies would profit from a robust workflow for mouse IgG glycome analysis from minute amounts of starting material, collected through a simple sampling procedure. For this purpose, we have developed a protocol for analysis of total N‐glycans of IgG isolated from mouse dried blood spots (DBS), which we report here. 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| subjects | Animals Biochemical Research Methods Biochemistry & Molecular Biology Biomarker Biomedical materials Blood Capillary gel electrophoresis laser‐induced fluorescence Chemistry Chemistry, Analytical Dried Blood Spot Testing Dried blood spots Electrophoresis Electrophoresis, Capillary Fluorescence Glycan Glycosylation IgG antibody IgG glycome Immunoglobulin G - blood Immunoglobulins Life Sciences & Biomedicine Mice Mouse Physical Sciences Polysaccharides - chemistry Sampling Science & Technology Workflow |
| title | N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots |
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