Pyrolyzed Photoresist Carbon Electrodes for Microchip Electrophoresis with Dual-Electrode Amperometric Detection

Pyrolyzed Photoresist Carbon Electrodes for Microchip Electrophoresis with Dual-Electrode Amperometric Detection

The fabrication and evaluation of pyrolyzed photoresist films (PPF) for microchip capillary electrophoresis (CE) with dual‐electrode electrochemical (EC) detection is described. The sensitivity, linearity, and reproducibility were evaluated using catecholamines and related compounds, including dopam...

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Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2005-07, Vol.17 (13), p.1153-1159
Hauptverfasser: Fischer, David J., Vandaveer IV, Walter R., Grigsby, Ryan J., Lunte, Susan M.
Format: Artikel
Sprache:eng
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Amperometric detection
Dual-electrode
Microchip capillary electrophoresis
Miniaturization
Pyrolyzed photoresist films
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container_end_page 1159
container_issue 13
container_start_page 1153
container_title Electroanalysis (New York, N.Y.)
container_volume 17
creator Fischer, David J.
Vandaveer IV, Walter R.
Grigsby, Ryan J.
Lunte, Susan M.
description The fabrication and evaluation of pyrolyzed photoresist films (PPF) for microchip capillary electrophoresis (CE) with dual‐electrode electrochemical (EC) detection is described. The sensitivity, linearity, and reproducibility were evaluated using catecholamines and related compounds, including dopamine (DA), 5‐hydroxyindole‐3‐acetic acid (5‐HIAA), ascorbic acid (AA), and catechol. Initial studies with DA show the response of the PPF electrodes to be linear between 25 and 500 μM (r2=0.999) with a limit of detection (LOD) of 5 μM (S/N=3) and sensitivity of 5.8 pA/μM. Selectivity was further enhanced by employing dual‐electrode detection in the series configuration for detection of species exhibiting chemically reversible redox reactions.
doi_str_mv 10.1002/elan.200503239
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source Wiley Online Library Journals Frontfile Complete
subjects Amperometric detection
Dual-electrode
Microchip capillary electrophoresis
Miniaturization
Pyrolyzed photoresist films
title Pyrolyzed Photoresist Carbon Electrodes for Microchip Electrophoresis with Dual-Electrode Amperometric Detection
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