An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

An inexpensive and easy to construct miniaturized biosensor is described for the determination of uric acid in biological fluids. The amperometric biosensor was prepared by using a carbon paste electrode prepared with uricase from Arthrobacter globiforms and tetracyanoquinodimethane as electron tran...

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Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2005-04, Vol.17 (8), p.701-705
Hauptverfasser: Dutra, R. F., Moreira, K. A., Oliveira, M. I. P., Araújo, A. N., Montenegro, M. C. B. S., Filho, J. L. L., Silva, V. L.
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Sprache:eng
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Zusammenfassung:An inexpensive and easy to construct miniaturized biosensor is described for the determination of uric acid in biological fluids. The amperometric biosensor was prepared by using a carbon paste electrode prepared with uricase from Arthrobacter globiforms and tetracyanoquinodimethane as electron transfer mediator. When incorporated into a flow‐injection system it was enabled to perform 50 measurements/h of uric acid in the analytical range of 1–100 μmol dm−3 with a relative standard deviation of 0.20% (n=14). The system was applied to human serum samples analysis providing good data correlation with those obtained by the reference spectrophotometric method. A linear relationship AM (μmol dm−3)=1.02 (±0.05) SP (μmol dm−3) −0.12 (±0.13) was obtained evidencing the absence of significant error. The constructed biosensor was successfully used for at least four months (250 assays) with only a 13% of decrease in the enzymatic activity.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200403142