Electroanalytical determination of peroxidases and laccases on carbon paste electrodes
Electrocatalytical reduction of hydrogen peroxide and oxygen was performed by horseradish peroxidase, recombinant Coprinus cinereus peroxidase, Polyporus pinsitus laccase and Botrytis cinerea laccase on carbon paste electrodes containing a paraffin soluble mediator. The electrocatalytical process pr...
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| Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 1997-02, Vol.9 (3), p.213-218 |
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| container_title | Electroanalysis (New York, N.Y.) |
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| creator | Kulys, Juozas Drungiliene, Alma Wollenberger, Ulla Krikstopaitis, Kastis Scheller, Frieder |
| description | Electrocatalytical reduction of hydrogen peroxide and oxygen was performed by horseradish peroxidase, recombinant Coprinus cinereus peroxidase, Polyporus pinsitus laccase and Botrytis cinerea laccase on carbon paste electrodes containing a paraffin soluble mediator. The electrocatalytical process proceeded at 0.0V (vs Ag/AgCl; in situ electrode). The electrode responses had maximum values at pH 6.4 and 5.5 for the horseradish peroxidase and laccases catalyzed reactions, respectively. The sensitivity for horseradish peroxidase and recombinant Coprinus cinereus peroxidase was 23.4 and 5.4 μC nM−1 cm−2, respectively. A detection limit of 7.5 fmol horseradish peroxidase could thus be achieved. Systems based on laccases showed lower sensitivity. The model of electrocatalytical reduction of hydrogen peroxide and oxygen included a permanent liberation of mediator from the carbon paste and it's biocatalytical conversion in the diffusion layer. The model was analyzed by comparing kinetic data in homogeneous solution with the electrode response. Mass transport effects were studied by using rotating carbon paste electrodes. |
| doi_str_mv | 10.1002/elan.1140090305 |
| format | Article |
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The electrocatalytical process proceeded at 0.0V (vs Ag/AgCl; in situ electrode). The electrode responses had maximum values at pH 6.4 and 5.5 for the horseradish peroxidase and laccases catalyzed reactions, respectively. The sensitivity for horseradish peroxidase and recombinant Coprinus cinereus peroxidase was 23.4 and 5.4 μC nM−1 cm−2, respectively. A detection limit of 7.5 fmol horseradish peroxidase could thus be achieved. Systems based on laccases showed lower sensitivity. The model of electrocatalytical reduction of hydrogen peroxide and oxygen included a permanent liberation of mediator from the carbon paste and it's biocatalytical conversion in the diffusion layer. The model was analyzed by comparing kinetic data in homogeneous solution with the electrode response. Mass transport effects were studied by using rotating carbon paste electrodes.</description><identifier>ISSN: 1040-0397</identifier><identifier>EISSN: 1521-4109</identifier><identifier>DOI: 10.1002/elan.1140090305</identifier><language>eng</language><publisher>Weinheim: VCH Verlagsgesellschaft mbH</publisher><subject>1-(N ; 1‐(N,N‐dimethylamine)‐4‐(4‐morpholine)benzene ; Botrytis cinerea laccase ; Carbon paste electrode ; Chemically modified electrode ; Coprinus cinereus peroxidase ; Graphite ; Horseradish peroxidase ; N-dimethylamine)-4-(4-morpholine)benzene ; Polyporus pinsitus laccase</subject><ispartof>Electroanalysis (New York, N.Y.), 1997-02, Vol.9 (3), p.213-218</ispartof><rights>Copyright © 1997 VCH Verlagsgesellschaft mbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3705-7a166ba8bf743738aabfcca982ffd2d5b0f7219e7db9445e343338e24d38f73f3</citedby><cites>FETCH-LOGICAL-c3705-7a166ba8bf743738aabfcca982ffd2d5b0f7219e7db9445e343338e24d38f73f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felan.1140090305$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felan.1140090305$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Kulys, Juozas</creatorcontrib><creatorcontrib>Drungiliene, Alma</creatorcontrib><creatorcontrib>Wollenberger, Ulla</creatorcontrib><creatorcontrib>Krikstopaitis, Kastis</creatorcontrib><creatorcontrib>Scheller, Frieder</creatorcontrib><title>Electroanalytical determination of peroxidases and laccases on carbon paste electrodes</title><title>Electroanalysis (New York, N.Y.)</title><addtitle>Electroanalysis</addtitle><description>Electrocatalytical reduction of hydrogen peroxide and oxygen was performed by horseradish peroxidase, recombinant Coprinus cinereus peroxidase, Polyporus pinsitus laccase and Botrytis cinerea laccase on carbon paste electrodes containing a paraffin soluble mediator. 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Mass transport effects were studied by using rotating carbon paste electrodes.</description><subject>1-(N</subject><subject>1‐(N,N‐dimethylamine)‐4‐(4‐morpholine)benzene</subject><subject>Botrytis cinerea laccase</subject><subject>Carbon paste electrode</subject><subject>Chemically modified electrode</subject><subject>Coprinus cinereus peroxidase</subject><subject>Graphite</subject><subject>Horseradish peroxidase</subject><subject>N-dimethylamine)-4-(4-morpholine)benzene</subject><subject>Polyporus pinsitus laccase</subject><issn>1040-0397</issn><issn>1521-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkEFLw0AQhRdRsFbPXvMH0s5mN9kET6W0qVAqYq3HZZKdhWialN2A9t-bGlE8eXozzPsezGPslsOEA0RTqrGZcC4BMhAQn7ERjyMeSg7ZeT-DhBBEpi7Zlfev0LsSmY3YblFT2bkWG6yPXVViHRjqyO2rBruqbYLWBgdy7Udl0JMPsDFBjWX5tfTnEl3RywF9RwENWYb8NbuwWHu6-dYxe14utvNVuH7I7-ezdVgKBXGokCdJgWlhlRRKpIiF7bOzNLLWRCYuwKqIZ6RMkUkZk5BCiJQiaURqlbBizKZDbula7x1ZfXDVHt1Rc9CnWvSpFv1bS0_cDcR7VdPxP7terGebP3Q40FX_78cPje5NJ0qoWL9scr17zPPldvekV-ITAhl4yQ</recordid><startdate>199702</startdate><enddate>199702</enddate><creator>Kulys, Juozas</creator><creator>Drungiliene, Alma</creator><creator>Wollenberger, Ulla</creator><creator>Krikstopaitis, Kastis</creator><creator>Scheller, Frieder</creator><general>VCH Verlagsgesellschaft mbH</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199702</creationdate><title>Electroanalytical determination of peroxidases and laccases on carbon paste electrodes</title><author>Kulys, Juozas ; Drungiliene, Alma ; Wollenberger, Ulla ; Krikstopaitis, Kastis ; Scheller, Frieder</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3705-7a166ba8bf743738aabfcca982ffd2d5b0f7219e7db9445e343338e24d38f73f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>1-(N</topic><topic>1‐(N,N‐dimethylamine)‐4‐(4‐morpholine)benzene</topic><topic>Botrytis cinerea laccase</topic><topic>Carbon paste electrode</topic><topic>Chemically modified electrode</topic><topic>Coprinus cinereus peroxidase</topic><topic>Graphite</topic><topic>Horseradish peroxidase</topic><topic>N-dimethylamine)-4-(4-morpholine)benzene</topic><topic>Polyporus pinsitus laccase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kulys, Juozas</creatorcontrib><creatorcontrib>Drungiliene, Alma</creatorcontrib><creatorcontrib>Wollenberger, Ulla</creatorcontrib><creatorcontrib>Krikstopaitis, Kastis</creatorcontrib><creatorcontrib>Scheller, Frieder</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><jtitle>Electroanalysis (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kulys, Juozas</au><au>Drungiliene, Alma</au><au>Wollenberger, Ulla</au><au>Krikstopaitis, Kastis</au><au>Scheller, Frieder</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroanalytical determination of peroxidases and laccases on carbon paste electrodes</atitle><jtitle>Electroanalysis (New York, N.Y.)</jtitle><addtitle>Electroanalysis</addtitle><date>1997-02</date><risdate>1997</risdate><volume>9</volume><issue>3</issue><spage>213</spage><epage>218</epage><pages>213-218</pages><issn>1040-0397</issn><eissn>1521-4109</eissn><abstract>Electrocatalytical reduction of hydrogen peroxide and oxygen was performed by horseradish peroxidase, recombinant Coprinus cinereus peroxidase, Polyporus pinsitus laccase and Botrytis cinerea laccase on carbon paste electrodes containing a paraffin soluble mediator. The electrocatalytical process proceeded at 0.0V (vs Ag/AgCl; in situ electrode). The electrode responses had maximum values at pH 6.4 and 5.5 for the horseradish peroxidase and laccases catalyzed reactions, respectively. The sensitivity for horseradish peroxidase and recombinant Coprinus cinereus peroxidase was 23.4 and 5.4 μC nM−1 cm−2, respectively. A detection limit of 7.5 fmol horseradish peroxidase could thus be achieved. Systems based on laccases showed lower sensitivity. The model of electrocatalytical reduction of hydrogen peroxide and oxygen included a permanent liberation of mediator from the carbon paste and it's biocatalytical conversion in the diffusion layer. The model was analyzed by comparing kinetic data in homogeneous solution with the electrode response. Mass transport effects were studied by using rotating carbon paste electrodes.</abstract><cop>Weinheim</cop><pub>VCH Verlagsgesellschaft mbH</pub><doi>10.1002/elan.1140090305</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Electroanalysis (New York, N.Y.), 1997-02, Vol.9 (3), p.213-218 |
| issn | 1040-0397 1521-4109 |
| language | eng |
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| source | Access via Wiley Online Library |
| subjects | 1-(N 1‐(N,N‐dimethylamine)‐4‐(4‐morpholine)benzene Botrytis cinerea laccase Carbon paste electrode Chemically modified electrode Coprinus cinereus peroxidase Graphite Horseradish peroxidase N-dimethylamine)-4-(4-morpholine)benzene Polyporus pinsitus laccase |
| title | Electroanalytical determination of peroxidases and laccases on carbon paste electrodes |
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