Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development
The thymus is an organ where T lymphocytes develop. Thymus development requires interactions of cells derived from three germ layers. However, the molecular mechanisms that control thymus development are not fully understood. To identify the genes that regulate thymus development, we previously carr...
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| Veröffentlicht in: | European journal of immunology 2009-09, Vol.39 (9), p.2606-2616 |
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| container_title | European journal of immunology |
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| creator | Iwanami, Norimasa Okada, Minoru Hoa, Vu Q Seo, Yasuhito Mitani, Hiroshi Sasaki, Takashi Shimizu, Nobuyoshi Kondoh, Hisato Furutani-Seiki, Makoto Takahama, Yousuke |
| description | The thymus is an organ where T lymphocytes develop. Thymus development requires interactions of cells derived from three germ layers. However, the molecular mechanisms that control thymus development are not fully understood. To identify the genes that regulate thymus development, we previously carried out a large-scale screening for ethylnitrosourea-induced mutagenesis using medaka, Oryzias latipes, and established a panel of recessive thymus-lacking mutants. Here we report the identification of three genes responsible for these mutations. We found that the mutations in KIAA1440, TRRAP, and SKIV2L2 caused the defects in distinct steps of thymus development. We also found that these genes were widely expressed in many organs and that the mutations in these genes caused defects in the development of various other organs. These results enabled us to identify previously unknown roles of widely expressed genes in medaka organ development. The possible reasons why thymus-defective teleost mutants could be used to identify widely expressed genes and future strategies to increase the likelihood of identifying genes that specifically regulate thymus development are discussed. |
| doi_str_mv | 10.1002/eji.200939362 |
| format | Article |
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Thymus development requires interactions of cells derived from three germ layers. However, the molecular mechanisms that control thymus development are not fully understood. To identify the genes that regulate thymus development, we previously carried out a large-scale screening for ethylnitrosourea-induced mutagenesis using medaka, Oryzias latipes, and established a panel of recessive thymus-lacking mutants. Here we report the identification of three genes responsible for these mutations. We found that the mutations in KIAA1440, TRRAP, and SKIV2L2 caused the defects in distinct steps of thymus development. We also found that these genes were widely expressed in many organs and that the mutations in these genes caused defects in the development of various other organs. These results enabled us to identify previously unknown roles of widely expressed genes in medaka organ development. The possible reasons why thymus-defective teleost mutants could be used to identify widely expressed genes and future strategies to increase the likelihood of identifying genes that specifically regulate thymus development are discussed.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.200939362</identifier><identifier>PMID: 19670383</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - physiology ; Alternative Splicing ; Animals ; Base Sequence ; Ethylnitrosourea - pharmacology ; Ethylnitrosourea‐induced mutagenesis ; Exons - genetics ; Medaka ; Molecular Sequence Data ; Mutation ; Nuclear Proteins - genetics ; Nuclear Proteins - physiology ; Organogenesis ; Organogenesis - genetics ; Oryzias - embryology ; Oryzias - genetics ; Oryzias - immunology ; Positional cloning ; RNA Helicases - genetics ; RNA Helicases - physiology ; Thymus ; Thymus Gland - embryology</subject><ispartof>European journal of immunology, 2009-09, Vol.39 (9), p.2606-2616</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4022-ce729b7136977f30fa5cd53353a2077ae360a2ed896218d4be922ff1c03097c63</citedby><cites>FETCH-LOGICAL-c4022-ce729b7136977f30fa5cd53353a2077ae360a2ed896218d4be922ff1c03097c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Feji.200939362$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Feji.200939362$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27903,27904,45553,45554,46388,46812</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19670383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwanami, Norimasa</creatorcontrib><creatorcontrib>Okada, Minoru</creatorcontrib><creatorcontrib>Hoa, Vu Q</creatorcontrib><creatorcontrib>Seo, Yasuhito</creatorcontrib><creatorcontrib>Mitani, Hiroshi</creatorcontrib><creatorcontrib>Sasaki, Takashi</creatorcontrib><creatorcontrib>Shimizu, Nobuyoshi</creatorcontrib><creatorcontrib>Kondoh, Hisato</creatorcontrib><creatorcontrib>Furutani-Seiki, Makoto</creatorcontrib><creatorcontrib>Takahama, Yousuke</creatorcontrib><title>Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>The thymus is an organ where T lymphocytes develop. Thymus development requires interactions of cells derived from three germ layers. However, the molecular mechanisms that control thymus development are not fully understood. To identify the genes that regulate thymus development, we previously carried out a large-scale screening for ethylnitrosourea-induced mutagenesis using medaka, Oryzias latipes, and established a panel of recessive thymus-lacking mutants. Here we report the identification of three genes responsible for these mutations. We found that the mutations in KIAA1440, TRRAP, and SKIV2L2 caused the defects in distinct steps of thymus development. We also found that these genes were widely expressed in many organs and that the mutations in these genes caused defects in the development of various other organs. These results enabled us to identify previously unknown roles of widely expressed genes in medaka organ development. The possible reasons why thymus-defective teleost mutants could be used to identify widely expressed genes and future strategies to increase the likelihood of identifying genes that specifically regulate thymus development are discussed.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adaptor Proteins, Signal Transducing - physiology</subject><subject>Alternative Splicing</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Ethylnitrosourea - pharmacology</subject><subject>Ethylnitrosourea‐induced mutagenesis</subject><subject>Exons - genetics</subject><subject>Medaka</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - physiology</subject><subject>Organogenesis</subject><subject>Organogenesis - genetics</subject><subject>Oryzias - embryology</subject><subject>Oryzias - genetics</subject><subject>Oryzias - immunology</subject><subject>Positional cloning</subject><subject>RNA Helicases - genetics</subject><subject>RNA Helicases - physiology</subject><subject>Thymus</subject><subject>Thymus Gland - embryology</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtO6zAQQC0EgvJYsgV_AIHxOLHrZYUK9FIJxGsbufEYjPKo4qSoi_vvN1dFwIrVSKMzR5rD2LGAcwGAF_QezhHASCMVbrGRyFAkqUjFNhsBiDRBM4Y9th_jOwyYyswu2xNGaZBjOWJ_p93buqxD1zax6VuySahdX5Djw77qY-LIU9GFFfGq72zdRR4c1V3wa942JUXeeH47m0xEmsIZf3p4mNyfcVs7_ng7e8E58lDzjkpqYseb9tXW3NGKymZZDZZDtuNtGenocx6w56vp0-VNMr-7nl1O5kmRAmJSkEaz0EIqo7WX4G1WuEzKTFoErS1JBRbJjY1CMXbpggyi96IACUYXSh6wZOMthjdjSz5ftqGy7ToXkP_PmA8Z86-MA3-y4Zf9oiL3TX92GwC9AT5CSevfbfn0z-yn-nRz6W2T29c2xPz5EUFIEEorRCX_Af7Qhrc</recordid><startdate>200909</startdate><enddate>200909</enddate><creator>Iwanami, Norimasa</creator><creator>Okada, Minoru</creator><creator>Hoa, Vu Q</creator><creator>Seo, Yasuhito</creator><creator>Mitani, Hiroshi</creator><creator>Sasaki, Takashi</creator><creator>Shimizu, Nobuyoshi</creator><creator>Kondoh, Hisato</creator><creator>Furutani-Seiki, Makoto</creator><creator>Takahama, Yousuke</creator><general>Wiley-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200909</creationdate><title>Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development</title><author>Iwanami, Norimasa ; Okada, Minoru ; Hoa, Vu Q ; Seo, Yasuhito ; Mitani, Hiroshi ; Sasaki, Takashi ; Shimizu, Nobuyoshi ; Kondoh, Hisato ; Furutani-Seiki, Makoto ; Takahama, Yousuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4022-ce729b7136977f30fa5cd53353a2077ae360a2ed896218d4be922ff1c03097c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adaptor Proteins, Signal Transducing - physiology</topic><topic>Alternative Splicing</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Ethylnitrosourea - pharmacology</topic><topic>Ethylnitrosourea‐induced mutagenesis</topic><topic>Exons - genetics</topic><topic>Medaka</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - physiology</topic><topic>Organogenesis</topic><topic>Organogenesis - genetics</topic><topic>Oryzias - embryology</topic><topic>Oryzias - genetics</topic><topic>Oryzias - immunology</topic><topic>Positional cloning</topic><topic>RNA Helicases - genetics</topic><topic>RNA Helicases - physiology</topic><topic>Thymus</topic><topic>Thymus Gland - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwanami, Norimasa</creatorcontrib><creatorcontrib>Okada, Minoru</creatorcontrib><creatorcontrib>Hoa, Vu Q</creatorcontrib><creatorcontrib>Seo, Yasuhito</creatorcontrib><creatorcontrib>Mitani, Hiroshi</creatorcontrib><creatorcontrib>Sasaki, Takashi</creatorcontrib><creatorcontrib>Shimizu, Nobuyoshi</creatorcontrib><creatorcontrib>Kondoh, Hisato</creatorcontrib><creatorcontrib>Furutani-Seiki, Makoto</creatorcontrib><creatorcontrib>Takahama, Yousuke</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwanami, Norimasa</au><au>Okada, Minoru</au><au>Hoa, Vu Q</au><au>Seo, Yasuhito</au><au>Mitani, Hiroshi</au><au>Sasaki, Takashi</au><au>Shimizu, Nobuyoshi</au><au>Kondoh, Hisato</au><au>Furutani-Seiki, Makoto</au><au>Takahama, Yousuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>2009-09</date><risdate>2009</risdate><volume>39</volume><issue>9</issue><spage>2606</spage><epage>2616</epage><pages>2606-2616</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>The thymus is an organ where T lymphocytes develop. Thymus development requires interactions of cells derived from three germ layers. However, the molecular mechanisms that control thymus development are not fully understood. To identify the genes that regulate thymus development, we previously carried out a large-scale screening for ethylnitrosourea-induced mutagenesis using medaka, Oryzias latipes, and established a panel of recessive thymus-lacking mutants. Here we report the identification of three genes responsible for these mutations. We found that the mutations in KIAA1440, TRRAP, and SKIV2L2 caused the defects in distinct steps of thymus development. We also found that these genes were widely expressed in many organs and that the mutations in these genes caused defects in the development of various other organs. These results enabled us to identify previously unknown roles of widely expressed genes in medaka organ development. The possible reasons why thymus-defective teleost mutants could be used to identify widely expressed genes and future strategies to increase the likelihood of identifying genes that specifically regulate thymus development are discussed.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>19670383</pmid><doi>10.1002/eji.200939362</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library; EZB-FREE-00999 freely available EZB journals |
| subjects | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - physiology Alternative Splicing Animals Base Sequence Ethylnitrosourea - pharmacology Ethylnitrosourea‐induced mutagenesis Exons - genetics Medaka Molecular Sequence Data Mutation Nuclear Proteins - genetics Nuclear Proteins - physiology Organogenesis Organogenesis - genetics Oryzias - embryology Oryzias - genetics Oryzias - immunology Positional cloning RNA Helicases - genetics RNA Helicases - physiology Thymus Thymus Gland - embryology |
| title | Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development |
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