Increase of intracellular calcium is the essential signal for the expression of CD40 ligand
CD40 ligand (CD40L) is present on activated but not on resting T cells. In contrast to the activation markers CD25 and CD71, a strong CD40L expression could be induced by calcium ionophore alone but not by phorbol 12‐myristate 13‐acetate (PMA). Ionomycin induced a very early mRNA and protein surface...
Gespeichert in:
| Veröffentlicht in: | European journal of immunology 1996-04, Vol.26 (4), p.846-850 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 850 |
|---|---|
| container_issue | 4 |
| container_start_page | 846 |
| container_title | European journal of immunology |
| container_volume | 26 |
| creator | Nüsslein, Hubert G. Frosch, Karl‐Heinz Woith, Walter Lane, Peter Kalden, Joachim R. Manger, Bernhard |
| description | CD40 ligand (CD40L) is present on activated but not on resting T cells. In contrast to the activation markers CD25 and CD71, a strong CD40L expression could be induced by calcium ionophore alone but not by phorbol 12‐myristate 13‐acetate (PMA). Ionomycin induced a very early mRNA and protein surface expression of CD40L within the first 2 h, whereas CD25 and CD71 did not appear earlier than 6 h after stimulation. The mitogens phytohemagglutinin and concanavalin A induced little CD40L, but together with PMA, a markedly increased CD40L expression was observed. In T cells stimulated with immobilized anti‐CD3, co‐stimulation with anti‐CD28 or PMA induced an earlier and higher maximal CD40L expression. CD40L expression of purified T cells was higher and more prolonged compared to that of T cells in unseparated peripheral blood mononuclear cells. We conclude that the expression of CD40L on T cells is profoundly different from other early activation markers with regard to signal requirements, kinetics and the role of accessory cells in the system. |
| doi_str_mv | 10.1002/eji.1830260418 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_eji_1830260418</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EJI1830260418</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4068-ba7f5ab0c5a8cc64e5d94a62c4f89d817bbae7e87ecb314441c15c1d542c15c83</originalsourceid><addsrcrecordid>eNqFkDFPwzAQhS0EKqWwsiH5D6TcuXbijKgUKKrEAhND5DiX4spNKrsV9N-TKhWwMb2T3r1v-Bi7RhgjgLillRujnoBIQaI-YUNUAhOJEk_ZEABlInIN5-wixhUA5KnKB2ygU6HyLBuy93ljA5lIvK25a7bBWPJ-503g1njrdmvuIt9-EKcYqdk643l0y6aLug198bUJXena5sCY3kvg3i1NU12ys9r4SFfHHLG3h9nr9ClZvDzOp3eLxEpIdVKarFamBKuMtjaVpKpcmlRYWeu80piVpaGMdEa2nKCUEi0qi5WS4nDoyYiNe64NbYyB6mIT3NqEfYFQHCQVnaTiV1I3uOkHm125purn_Wil6_O-_3Se9v_Qitnz_A_7G4Dvc90</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Increase of intracellular calcium is the essential signal for the expression of CD40 ligand</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Nüsslein, Hubert G. ; Frosch, Karl‐Heinz ; Woith, Walter ; Lane, Peter ; Kalden, Joachim R. ; Manger, Bernhard</creator><creatorcontrib>Nüsslein, Hubert G. ; Frosch, Karl‐Heinz ; Woith, Walter ; Lane, Peter ; Kalden, Joachim R. ; Manger, Bernhard</creatorcontrib><description>CD40 ligand (CD40L) is present on activated but not on resting T cells. In contrast to the activation markers CD25 and CD71, a strong CD40L expression could be induced by calcium ionophore alone but not by phorbol 12‐myristate 13‐acetate (PMA). Ionomycin induced a very early mRNA and protein surface expression of CD40L within the first 2 h, whereas CD25 and CD71 did not appear earlier than 6 h after stimulation. The mitogens phytohemagglutinin and concanavalin A induced little CD40L, but together with PMA, a markedly increased CD40L expression was observed. In T cells stimulated with immobilized anti‐CD3, co‐stimulation with anti‐CD28 or PMA induced an earlier and higher maximal CD40L expression. CD40L expression of purified T cells was higher and more prolonged compared to that of T cells in unseparated peripheral blood mononuclear cells. We conclude that the expression of CD40L on T cells is profoundly different from other early activation markers with regard to signal requirements, kinetics and the role of accessory cells in the system.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.1830260418</identifier><identifier>PMID: 8625977</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - pharmacology ; Antigens, CD - analysis ; Antigens, Differentiation, B-Lymphocyte - analysis ; Calcium - physiology ; Calcium influx ; CD28 Antigens - immunology ; CD3 Complex - immunology ; CD40 Ligand ; Cell Separation ; Cyclosporine - pharmacology ; Egtazic Acid - pharmacology ; Enzyme Activation ; Gene Expression Regulation - physiology ; Humans ; Intracellular Fluid ; Ionomycin - pharmacology ; Ionophores - pharmacology ; Lymphocyte Activation - drug effects ; Membrane Glycoproteins - biosynthesis ; Membrane Glycoproteins - genetics ; Mitogens - pharmacology ; Protein Kinase C - metabolism ; Receptors, Interleukin-2 - analysis ; Receptors, Transferrin ; T cell activation ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>European journal of immunology, 1996-04, Vol.26 (4), p.846-850</ispartof><rights>Copyright © 1996 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4068-ba7f5ab0c5a8cc64e5d94a62c4f89d817bbae7e87ecb314441c15c1d542c15c83</citedby><cites>FETCH-LOGICAL-c4068-ba7f5ab0c5a8cc64e5d94a62c4f89d817bbae7e87ecb314441c15c1d542c15c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Feji.1830260418$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Feji.1830260418$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8625977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nüsslein, Hubert G.</creatorcontrib><creatorcontrib>Frosch, Karl‐Heinz</creatorcontrib><creatorcontrib>Woith, Walter</creatorcontrib><creatorcontrib>Lane, Peter</creatorcontrib><creatorcontrib>Kalden, Joachim R.</creatorcontrib><creatorcontrib>Manger, Bernhard</creatorcontrib><title>Increase of intracellular calcium is the essential signal for the expression of CD40 ligand</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>CD40 ligand (CD40L) is present on activated but not on resting T cells. In contrast to the activation markers CD25 and CD71, a strong CD40L expression could be induced by calcium ionophore alone but not by phorbol 12‐myristate 13‐acetate (PMA). Ionomycin induced a very early mRNA and protein surface expression of CD40L within the first 2 h, whereas CD25 and CD71 did not appear earlier than 6 h after stimulation. The mitogens phytohemagglutinin and concanavalin A induced little CD40L, but together with PMA, a markedly increased CD40L expression was observed. In T cells stimulated with immobilized anti‐CD3, co‐stimulation with anti‐CD28 or PMA induced an earlier and higher maximal CD40L expression. CD40L expression of purified T cells was higher and more prolonged compared to that of T cells in unseparated peripheral blood mononuclear cells. We conclude that the expression of CD40L on T cells is profoundly different from other early activation markers with regard to signal requirements, kinetics and the role of accessory cells in the system.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, Differentiation, B-Lymphocyte - analysis</subject><subject>Calcium - physiology</subject><subject>Calcium influx</subject><subject>CD28 Antigens - immunology</subject><subject>CD3 Complex - immunology</subject><subject>CD40 Ligand</subject><subject>Cell Separation</subject><subject>Cyclosporine - pharmacology</subject><subject>Egtazic Acid - pharmacology</subject><subject>Enzyme Activation</subject><subject>Gene Expression Regulation - physiology</subject><subject>Humans</subject><subject>Intracellular Fluid</subject><subject>Ionomycin - pharmacology</subject><subject>Ionophores - pharmacology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Mitogens - pharmacology</subject><subject>Protein Kinase C - metabolism</subject><subject>Receptors, Interleukin-2 - analysis</subject><subject>Receptors, Transferrin</subject><subject>T cell activation</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAQhS0EKqWwsiH5D6TcuXbijKgUKKrEAhND5DiX4spNKrsV9N-TKhWwMb2T3r1v-Bi7RhgjgLillRujnoBIQaI-YUNUAhOJEk_ZEABlInIN5-wixhUA5KnKB2ygU6HyLBuy93ljA5lIvK25a7bBWPJ-503g1njrdmvuIt9-EKcYqdk643l0y6aLug198bUJXena5sCY3kvg3i1NU12ys9r4SFfHHLG3h9nr9ClZvDzOp3eLxEpIdVKarFamBKuMtjaVpKpcmlRYWeu80piVpaGMdEa2nKCUEi0qi5WS4nDoyYiNe64NbYyB6mIT3NqEfYFQHCQVnaTiV1I3uOkHm125purn_Wil6_O-_3Se9v_Qitnz_A_7G4Dvc90</recordid><startdate>199604</startdate><enddate>199604</enddate><creator>Nüsslein, Hubert G.</creator><creator>Frosch, Karl‐Heinz</creator><creator>Woith, Walter</creator><creator>Lane, Peter</creator><creator>Kalden, Joachim R.</creator><creator>Manger, Bernhard</creator><general>WILEY‐VCH Verlag GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199604</creationdate><title>Increase of intracellular calcium is the essential signal for the expression of CD40 ligand</title><author>Nüsslein, Hubert G. ; Frosch, Karl‐Heinz ; Woith, Walter ; Lane, Peter ; Kalden, Joachim R. ; Manger, Bernhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4068-ba7f5ab0c5a8cc64e5d94a62c4f89d817bbae7e87ecb314441c15c1d542c15c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, Differentiation, B-Lymphocyte - analysis</topic><topic>Calcium - physiology</topic><topic>Calcium influx</topic><topic>CD28 Antigens - immunology</topic><topic>CD3 Complex - immunology</topic><topic>CD40 Ligand</topic><topic>Cell Separation</topic><topic>Cyclosporine - pharmacology</topic><topic>Egtazic Acid - pharmacology</topic><topic>Enzyme Activation</topic><topic>Gene Expression Regulation - physiology</topic><topic>Humans</topic><topic>Intracellular Fluid</topic><topic>Ionomycin - pharmacology</topic><topic>Ionophores - pharmacology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Mitogens - pharmacology</topic><topic>Protein Kinase C - metabolism</topic><topic>Receptors, Interleukin-2 - analysis</topic><topic>Receptors, Transferrin</topic><topic>T cell activation</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nüsslein, Hubert G.</creatorcontrib><creatorcontrib>Frosch, Karl‐Heinz</creatorcontrib><creatorcontrib>Woith, Walter</creatorcontrib><creatorcontrib>Lane, Peter</creatorcontrib><creatorcontrib>Kalden, Joachim R.</creatorcontrib><creatorcontrib>Manger, Bernhard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nüsslein, Hubert G.</au><au>Frosch, Karl‐Heinz</au><au>Woith, Walter</au><au>Lane, Peter</au><au>Kalden, Joachim R.</au><au>Manger, Bernhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increase of intracellular calcium is the essential signal for the expression of CD40 ligand</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>1996-04</date><risdate>1996</risdate><volume>26</volume><issue>4</issue><spage>846</spage><epage>850</epage><pages>846-850</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>CD40 ligand (CD40L) is present on activated but not on resting T cells. In contrast to the activation markers CD25 and CD71, a strong CD40L expression could be induced by calcium ionophore alone but not by phorbol 12‐myristate 13‐acetate (PMA). Ionomycin induced a very early mRNA and protein surface expression of CD40L within the first 2 h, whereas CD25 and CD71 did not appear earlier than 6 h after stimulation. The mitogens phytohemagglutinin and concanavalin A induced little CD40L, but together with PMA, a markedly increased CD40L expression was observed. In T cells stimulated with immobilized anti‐CD3, co‐stimulation with anti‐CD28 or PMA induced an earlier and higher maximal CD40L expression. CD40L expression of purified T cells was higher and more prolonged compared to that of T cells in unseparated peripheral blood mononuclear cells. We conclude that the expression of CD40L on T cells is profoundly different from other early activation markers with regard to signal requirements, kinetics and the role of accessory cells in the system.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>8625977</pmid><doi>10.1002/eji.1830260418</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2980 |
| ispartof | European journal of immunology, 1996-04, Vol.26 (4), p.846-850 |
| issn | 0014-2980 1521-4141 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_eji_1830260418 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Antigens, CD - analysis Antigens, Differentiation, B-Lymphocyte - analysis Calcium - physiology Calcium influx CD28 Antigens - immunology CD3 Complex - immunology CD40 Ligand Cell Separation Cyclosporine - pharmacology Egtazic Acid - pharmacology Enzyme Activation Gene Expression Regulation - physiology Humans Intracellular Fluid Ionomycin - pharmacology Ionophores - pharmacology Lymphocyte Activation - drug effects Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - genetics Mitogens - pharmacology Protein Kinase C - metabolism Receptors, Interleukin-2 - analysis Receptors, Transferrin T cell activation T-Lymphocytes - drug effects T-Lymphocytes - immunology T-Lymphocytes - metabolism Tetradecanoylphorbol Acetate - pharmacology |
| title | Increase of intracellular calcium is the essential signal for the expression of CD40 ligand |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A37%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increase%20of%20intracellular%20calcium%20is%20the%20essential%20signal%20for%20the%20expression%20of%20CD40%20ligand&rft.jtitle=European%20journal%20of%20immunology&rft.au=N%C3%BCsslein,%20Hubert%20G.&rft.date=1996-04&rft.volume=26&rft.issue=4&rft.spage=846&rft.epage=850&rft.pages=846-850&rft.issn=0014-2980&rft.eissn=1521-4141&rft_id=info:doi/10.1002/eji.1830260418&rft_dat=%3Cwiley_cross%3EEJI1830260418%3C/wiley_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/8625977&rfr_iscdi=true |