Flow-cytometric measurement of respiratory burst in rat polymorphonuclear granulocytes: Comparison of four cell preparation procedures, and concentration-response evaluation of soluble stimulants

Polymorphonuclear neutrophils (PMNs) contribute to organ injury in sepsis, stroke, and other diseases. Evaluation of the oxidative burst by flow cytometry (FCM) is frequently applied to examine PMN status in humans, but rarely in rats. We established a method to assess granulocyte activation in rats...

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Veröffentlicht in:	Cytometry. Part A 2008-07, Vol.73A (7), p.643-650
Hauptverfasser:	Bitzinger, Diane I, Schlachetzki, Felix, Lindner, Regina, Trabold, Benedikt, Dittmar, Michael S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Separation - methods concentration–response relationship flow cytometry Flow Cytometry - methods Granulocytes - cytology lipopolysaccharides Lipopolysaccharides - metabolism Male N-Formylmethionine Leucyl-Phenylalanine - metabolism neutrophils Neutrophils - cytology N‐formylmethionine leucyl‐phenylalanine phorbol‐12‐myristate‐13‐acetate Rats Rats, Wistar Respiratory Burst Solubility Tetradecanoylphorbol Acetate - metabolism Tumor Necrosis Factor-alpha - metabolism tumor necrosis factor‐α
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