HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection
BACKGROUND: PIWI protein family was found to play an important role in stem cell self‐renewal. Overexpression of HIWI, the human homolog of PIWI family proteins, was found in several solid tumors, although the role of HIWI in hepatocellular carcinoma (HCC) and its prognostic value remain unclear. ME...
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container_title | Cancer |
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creator | Zhao, Yi‐Ming Zhou, Jia‐Min Wang, Long‐Rong He, Hong‐Wei Wang, Xi‐Long Tao, Zhong‐Hua Sun, Hui‐Chuan Wu, Wei‐Zhong Fan, Jia Tang, Zhao‐You Wang, Lu |
description | BACKGROUND:
PIWI protein family was found to play an important role in stem cell self‐renewal. Overexpression of HIWI, the human homolog of PIWI family proteins, was found in several solid tumors, although the role of HIWI in hepatocellular carcinoma (HCC) and its prognostic value remain unclear.
METHODS:
HIWI expression was measured in stepwise metastatic HCC cell lines (HCCLM3, MHCC97H, MHCC97L, SMMC7721, and HepG2), the normal liver cell line (L02), and HCC tissue samples (n = 20). Proliferation and invasion were investigated in HCC cell lines undergoing HIWI target small interfering RNA transfection. Also explored was HIWI expression in HCC tissue microarrays (n = 168) for survival analysis.
RESULTS:
Levels of HIWI protein and mRNA were up‐regulated in highly metastatic HCC cell lines (HCCLM3, MHCC97H, and MHCC97L), whereas their proliferation and invasion significantly decreased after depletion of HIWI. Intratumoral HIWI expression was higher than that of peritumoral tissue (P < .001) and positively associated with proliferating cell nuclear antigen expression (P < .001). Positive expression of intratumoral HIWI was associated with larger tumor size (P = .047) and intrahepatic metastasis (P = .027) and was an independent risk factor for overall survival (P = .007) and recurrence‐free survival (P = .036), particularly in patients with low serum α‐fetoprotein and low Edmondson‐Steiner grade.
CONCLUSIONS:
HIWI may play a key role in HCC proliferation and metastasis and can be a potential prognostic factor for HCC after curative resection, particularly with well‐differentiated HCC. Cancer 2011. © 2011 American Cancer Society.
HIWI may be a useful prognostic factor for hepatocellular carcinoma (HCC)—particularly with well‐differentiated HCC—after curative resection and may be involved in tumor proliferation and metastasis. |
doi_str_mv | 10.1002/cncr.26524 |
format | Article |
fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_cncr_26524</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CNCR26524</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3954-2cfed287f6cf90d71c81842a8c15e3e3f96063f97b9f91d46ae39664a09089d83</originalsourceid><addsrcrecordid>eNp90E9LwzAYBvAgipvTix9AcvEiVPOnaZOjDHWDoSCK3kqWvnGRri1J59i3N1un3rzk5eH9kRcehM4puaaEsBtTG3_NMsHSAzSkROUJoSk7RENCiExEyt8H6CSEzxhzJvgxGjCqpMqlGKJyMn2bYhewDqExTndQ4rXrFrj1zUfdhLhxNW5156DuQr9aQMyNgapaVdpjo71xdbPUWNsOYl75yL8AewhgOtfUp-jI6irA2X6O0Ov93ct4ksyeHqbj21liuBJpwoyFksncZsYqUubUSCpTpqWhAjhwqzKSxTefK6tomWYauMqyVBNFpColH6Gr_l_jmxA82KL1bqn9pqCk2FZVbKsqdlVFfNHjdjVfQvlLf7qJ4HIPdDC6sl7XxoU_JyQX6c7R3q1dBZt_Thbjx_Fzf_wbX4WChA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Open Access</source><source>Alma/SFX Local Collection</source><creator>Zhao, Yi‐Ming ; Zhou, Jia‐Min ; Wang, Long‐Rong ; He, Hong‐Wei ; Wang, Xi‐Long ; Tao, Zhong‐Hua ; Sun, Hui‐Chuan ; Wu, Wei‐Zhong ; Fan, Jia ; Tang, Zhao‐You ; Wang, Lu</creator><creatorcontrib>Zhao, Yi‐Ming ; Zhou, Jia‐Min ; Wang, Long‐Rong ; He, Hong‐Wei ; Wang, Xi‐Long ; Tao, Zhong‐Hua ; Sun, Hui‐Chuan ; Wu, Wei‐Zhong ; Fan, Jia ; Tang, Zhao‐You ; Wang, Lu</creatorcontrib><description>BACKGROUND:
PIWI protein family was found to play an important role in stem cell self‐renewal. Overexpression of HIWI, the human homolog of PIWI family proteins, was found in several solid tumors, although the role of HIWI in hepatocellular carcinoma (HCC) and its prognostic value remain unclear.
METHODS:
HIWI expression was measured in stepwise metastatic HCC cell lines (HCCLM3, MHCC97H, MHCC97L, SMMC7721, and HepG2), the normal liver cell line (L02), and HCC tissue samples (n = 20). Proliferation and invasion were investigated in HCC cell lines undergoing HIWI target small interfering RNA transfection. Also explored was HIWI expression in HCC tissue microarrays (n = 168) for survival analysis.
RESULTS:
Levels of HIWI protein and mRNA were up‐regulated in highly metastatic HCC cell lines (HCCLM3, MHCC97H, and MHCC97L), whereas their proliferation and invasion significantly decreased after depletion of HIWI. Intratumoral HIWI expression was higher than that of peritumoral tissue (P < .001) and positively associated with proliferating cell nuclear antigen expression (P < .001). Positive expression of intratumoral HIWI was associated with larger tumor size (P = .047) and intrahepatic metastasis (P = .027) and was an independent risk factor for overall survival (P = .007) and recurrence‐free survival (P = .036), particularly in patients with low serum α‐fetoprotein and low Edmondson‐Steiner grade.
CONCLUSIONS:
HIWI may play a key role in HCC proliferation and metastasis and can be a potential prognostic factor for HCC after curative resection, particularly with well‐differentiated HCC. Cancer 2011. © 2011 American Cancer Society.
HIWI may be a useful prognostic factor for hepatocellular carcinoma (HCC)—particularly with well‐differentiated HCC—after curative resection and may be involved in tumor proliferation and metastasis.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/cncr.26524</identifier><identifier>PMID: 21989785</identifier><identifier>CODEN: CANCAR</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; alpha-Fetoproteins - analysis ; Argonaute Proteins - analysis ; Argonaute Proteins - physiology ; Biological and medical sciences ; Carcinoma, Hepatocellular - mortality ; Carcinoma, Hepatocellular - pathology ; Carcinoma, Hepatocellular - surgery ; Cell Line, Tumor ; Cell Proliferation ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; hepatocellular carcinoma ; HIWI ; Humans ; Immunohistochemistry ; Liver Neoplasms - mortality ; Liver Neoplasms - pathology ; Liver Neoplasms - surgery ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; metastasis ; Middle Aged ; Neoplasm Invasiveness ; Prognosis ; Proliferating Cell Nuclear Antigen - analysis ; proliferation ; Tumors</subject><ispartof>Cancer, 2012-05, Vol.118 (10), p.2708-2717</ispartof><rights>Copyright © 2011 American Cancer Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 American Cancer Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3954-2cfed287f6cf90d71c81842a8c15e3e3f96063f97b9f91d46ae39664a09089d83</citedby><cites>FETCH-LOGICAL-c3954-2cfed287f6cf90d71c81842a8c15e3e3f96063f97b9f91d46ae39664a09089d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcncr.26524$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcncr.26524$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25835485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21989785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Yi‐Ming</creatorcontrib><creatorcontrib>Zhou, Jia‐Min</creatorcontrib><creatorcontrib>Wang, Long‐Rong</creatorcontrib><creatorcontrib>He, Hong‐Wei</creatorcontrib><creatorcontrib>Wang, Xi‐Long</creatorcontrib><creatorcontrib>Tao, Zhong‐Hua</creatorcontrib><creatorcontrib>Sun, Hui‐Chuan</creatorcontrib><creatorcontrib>Wu, Wei‐Zhong</creatorcontrib><creatorcontrib>Fan, Jia</creatorcontrib><creatorcontrib>Tang, Zhao‐You</creatorcontrib><creatorcontrib>Wang, Lu</creatorcontrib><title>HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection</title><title>Cancer</title><addtitle>Cancer</addtitle><description>BACKGROUND:
PIWI protein family was found to play an important role in stem cell self‐renewal. Overexpression of HIWI, the human homolog of PIWI family proteins, was found in several solid tumors, although the role of HIWI in hepatocellular carcinoma (HCC) and its prognostic value remain unclear.
METHODS:
HIWI expression was measured in stepwise metastatic HCC cell lines (HCCLM3, MHCC97H, MHCC97L, SMMC7721, and HepG2), the normal liver cell line (L02), and HCC tissue samples (n = 20). Proliferation and invasion were investigated in HCC cell lines undergoing HIWI target small interfering RNA transfection. Also explored was HIWI expression in HCC tissue microarrays (n = 168) for survival analysis.
RESULTS:
Levels of HIWI protein and mRNA were up‐regulated in highly metastatic HCC cell lines (HCCLM3, MHCC97H, and MHCC97L), whereas their proliferation and invasion significantly decreased after depletion of HIWI. Intratumoral HIWI expression was higher than that of peritumoral tissue (P < .001) and positively associated with proliferating cell nuclear antigen expression (P < .001). Positive expression of intratumoral HIWI was associated with larger tumor size (P = .047) and intrahepatic metastasis (P = .027) and was an independent risk factor for overall survival (P = .007) and recurrence‐free survival (P = .036), particularly in patients with low serum α‐fetoprotein and low Edmondson‐Steiner grade.
CONCLUSIONS:
HIWI may play a key role in HCC proliferation and metastasis and can be a potential prognostic factor for HCC after curative resection, particularly with well‐differentiated HCC. Cancer 2011. © 2011 American Cancer Society.
HIWI may be a useful prognostic factor for hepatocellular carcinoma (HCC)—particularly with well‐differentiated HCC—after curative resection and may be involved in tumor proliferation and metastasis.</description><subject>Adult</subject><subject>Aged</subject><subject>alpha-Fetoproteins - analysis</subject><subject>Argonaute Proteins - analysis</subject><subject>Argonaute Proteins - physiology</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Hepatocellular - mortality</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - surgery</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>hepatocellular carcinoma</subject><subject>HIWI</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Liver Neoplasms - mortality</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - surgery</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>metastasis</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Prognosis</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>proliferation</subject><subject>Tumors</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E9LwzAYBvAgipvTix9AcvEiVPOnaZOjDHWDoSCK3kqWvnGRri1J59i3N1un3rzk5eH9kRcehM4puaaEsBtTG3_NMsHSAzSkROUJoSk7RENCiExEyt8H6CSEzxhzJvgxGjCqpMqlGKJyMn2bYhewDqExTndQ4rXrFrj1zUfdhLhxNW5156DuQr9aQMyNgapaVdpjo71xdbPUWNsOYl75yL8AewhgOtfUp-jI6irA2X6O0Ov93ct4ksyeHqbj21liuBJpwoyFksncZsYqUubUSCpTpqWhAjhwqzKSxTefK6tomWYauMqyVBNFpColH6Gr_l_jmxA82KL1bqn9pqCk2FZVbKsqdlVFfNHjdjVfQvlLf7qJ4HIPdDC6sl7XxoU_JyQX6c7R3q1dBZt_Thbjx_Fzf_wbX4WChA</recordid><startdate>20120515</startdate><enddate>20120515</enddate><creator>Zhao, Yi‐Ming</creator><creator>Zhou, Jia‐Min</creator><creator>Wang, Long‐Rong</creator><creator>He, Hong‐Wei</creator><creator>Wang, Xi‐Long</creator><creator>Tao, Zhong‐Hua</creator><creator>Sun, Hui‐Chuan</creator><creator>Wu, Wei‐Zhong</creator><creator>Fan, Jia</creator><creator>Tang, Zhao‐You</creator><creator>Wang, Lu</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20120515</creationdate><title>HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection</title><author>Zhao, Yi‐Ming ; Zhou, Jia‐Min ; Wang, Long‐Rong ; He, Hong‐Wei ; Wang, Xi‐Long ; Tao, Zhong‐Hua ; Sun, Hui‐Chuan ; Wu, Wei‐Zhong ; Fan, Jia ; Tang, Zhao‐You ; Wang, Lu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3954-2cfed287f6cf90d71c81842a8c15e3e3f96063f97b9f91d46ae39664a09089d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>alpha-Fetoproteins - analysis</topic><topic>Argonaute Proteins - analysis</topic><topic>Argonaute Proteins - physiology</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Hepatocellular - mortality</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Carcinoma, Hepatocellular - surgery</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>hepatocellular carcinoma</topic><topic>HIWI</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Liver Neoplasms - mortality</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - surgery</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>metastasis</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Prognosis</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>proliferation</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yi‐Ming</creatorcontrib><creatorcontrib>Zhou, Jia‐Min</creatorcontrib><creatorcontrib>Wang, Long‐Rong</creatorcontrib><creatorcontrib>He, Hong‐Wei</creatorcontrib><creatorcontrib>Wang, Xi‐Long</creatorcontrib><creatorcontrib>Tao, Zhong‐Hua</creatorcontrib><creatorcontrib>Sun, Hui‐Chuan</creatorcontrib><creatorcontrib>Wu, Wei‐Zhong</creatorcontrib><creatorcontrib>Fan, Jia</creatorcontrib><creatorcontrib>Tang, Zhao‐You</creatorcontrib><creatorcontrib>Wang, Lu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yi‐Ming</au><au>Zhou, Jia‐Min</au><au>Wang, Long‐Rong</au><au>He, Hong‐Wei</au><au>Wang, Xi‐Long</au><au>Tao, Zhong‐Hua</au><au>Sun, Hui‐Chuan</au><au>Wu, Wei‐Zhong</au><au>Fan, Jia</au><au>Tang, Zhao‐You</au><au>Wang, Lu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>2012-05-15</date><risdate>2012</risdate><volume>118</volume><issue>10</issue><spage>2708</spage><epage>2717</epage><pages>2708-2717</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><coden>CANCAR</coden><abstract>BACKGROUND:
PIWI protein family was found to play an important role in stem cell self‐renewal. Overexpression of HIWI, the human homolog of PIWI family proteins, was found in several solid tumors, although the role of HIWI in hepatocellular carcinoma (HCC) and its prognostic value remain unclear.
METHODS:
HIWI expression was measured in stepwise metastatic HCC cell lines (HCCLM3, MHCC97H, MHCC97L, SMMC7721, and HepG2), the normal liver cell line (L02), and HCC tissue samples (n = 20). Proliferation and invasion were investigated in HCC cell lines undergoing HIWI target small interfering RNA transfection. Also explored was HIWI expression in HCC tissue microarrays (n = 168) for survival analysis.
RESULTS:
Levels of HIWI protein and mRNA were up‐regulated in highly metastatic HCC cell lines (HCCLM3, MHCC97H, and MHCC97L), whereas their proliferation and invasion significantly decreased after depletion of HIWI. Intratumoral HIWI expression was higher than that of peritumoral tissue (P < .001) and positively associated with proliferating cell nuclear antigen expression (P < .001). Positive expression of intratumoral HIWI was associated with larger tumor size (P = .047) and intrahepatic metastasis (P = .027) and was an independent risk factor for overall survival (P = .007) and recurrence‐free survival (P = .036), particularly in patients with low serum α‐fetoprotein and low Edmondson‐Steiner grade.
CONCLUSIONS:
HIWI may play a key role in HCC proliferation and metastasis and can be a potential prognostic factor for HCC after curative resection, particularly with well‐differentiated HCC. Cancer 2011. © 2011 American Cancer Society.
HIWI may be a useful prognostic factor for hepatocellular carcinoma (HCC)—particularly with well‐differentiated HCC—after curative resection and may be involved in tumor proliferation and metastasis.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21989785</pmid><doi>10.1002/cncr.26524</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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source | Wiley-Blackwell Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Wiley Open Access; Alma/SFX Local Collection |
subjects | Adult Aged alpha-Fetoproteins - analysis Argonaute Proteins - analysis Argonaute Proteins - physiology Biological and medical sciences Carcinoma, Hepatocellular - mortality Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - surgery Cell Line, Tumor Cell Proliferation Female Gastroenterology. Liver. Pancreas. Abdomen hepatocellular carcinoma HIWI Humans Immunohistochemistry Liver Neoplasms - mortality Liver Neoplasms - pathology Liver Neoplasms - surgery Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences metastasis Middle Aged Neoplasm Invasiveness Prognosis Proliferating Cell Nuclear Antigen - analysis proliferation Tumors |
title | HIWI is associated with prognosis in patients with hepatocellular carcinoma after curative resection |
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