Resolution of the Absorbance and CD Spectra and Formation Constants of the Complexes between Human Serum Albumin and Methyl Orange

Difference absorbance and circular dichroism techniques show that two complexes are formed between human serum albumin (HSA) and Methyl Orange (MO). The stoichiometries of the two HSA‐MO complexes (1:1 (C1) and 1:2 (C2)), their association constants (K1, 1 = 2.32 (0.18) × 105 M−1 and K1, 2 = 1.12 (0...

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Veröffentlicht in:	Chemistry : a European journal 1996-02, Vol.2 (2), p.149-156
Hauptverfasser:	Ambrosetti, R., Bianchini, R., Fisichella, S., Fichera, M., Zandomeneghi, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	azo dyes circular dichroism data fitting formation constants protein complexation
Online-Zugang:	Volltext
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