Front Cover: Re‐Programming and Optimization of a L‐Proline cis‐4‐Hydroxylase for the cis‐3‐Halogenation of its Native Substrate (ChemCatChem 18/2021)
The Front Cover represents the discovery of a novel path during a scientist′s journey towards the biocatalytic incorporation of chlorine into L‐proline. In their Communication, A. Papadopoulou et al describe the remodeling of an Fe/αKG hydroxylase into a halogenase utilizing the tools of protein eng...
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| Veröffentlicht in: | ChemCatChem 2021-09, Vol.13 (18), p.3907-3907 |
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| container_issue | 18 |
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| container_title | ChemCatChem |
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| creator | Papadopoulou, Athena Meierhofer, Jasmin Meyer, Fabian Hayashi, Takahiro Schneider, Samuel Sager, Emine Buller, Rebecca |
| description | The Front Cover represents the discovery of a novel path during a scientist′s journey towards the biocatalytic incorporation of chlorine into L‐proline. In their Communication, A. Papadopoulou et al describe the remodeling of an Fe/αKG hydroxylase into a halogenase utilizing the tools of protein engineering. By employing several rounds of directed evolution, a remodeled halogenase variant with a 98‐fold improved apparent kcat/Km was generated. The reprogrammed biocatalyst is the first example of a stereo‐ and regioselective halogenase derivatizing L‐proline at the C3 position and highlights the possibility to successfully access the diverse chemistries of Fe/αKG‐dependent dioxygenases to expand the biocatalytic toolbox. More information can be found in the Communication by A. Papadopoulou et al. |
| doi_str_mv | 10.1002/cctc.202101200 |
| format | Article |
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In their Communication, A. Papadopoulou et al describe the remodeling of an Fe/αKG hydroxylase into a halogenase utilizing the tools of protein engineering. By employing several rounds of directed evolution, a remodeled halogenase variant with a 98‐fold improved apparent kcat/Km was generated. The reprogrammed biocatalyst is the first example of a stereo‐ and regioselective halogenase derivatizing L‐proline at the C3 position and highlights the possibility to successfully access the diverse chemistries of Fe/αKG‐dependent dioxygenases to expand the biocatalytic toolbox. More information can be found in the Communication by A. 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| identifier | ISSN: 1867-3880 |
| ispartof | ChemCatChem, 2021-09, Vol.13 (18), p.3907-3907 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | biocatalysis C−H functionalization directed evolution enzymatic halogenation α-ketoglutarate dependent oxygenases |
| title | Front Cover: Re‐Programming and Optimization of a L‐Proline cis‐4‐Hydroxylase for the cis‐3‐Halogenation of its Native Substrate (ChemCatChem 18/2021) |
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