Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection
Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (E...
Gespeichert in:
| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2022-09, Vol.23 (17), p.e202200346 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 17 |
| container_start_page | e202200346 |
| container_title | Chembiochem : a European journal of chemical biology |
| container_volume | 23 |
| creator | Bhardwaj, Sheetal K Knaus, Tanja Garcia, Amanda Yan, Ning Mutti, Francesco G |
| description | Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (ERGO) deposited on indium tin oxide (ITO) and polyethylene terephthalate (PET) layers. XRD, SEM, AFM, FT-IR and Raman characterization of the sensor confirmed its structural integrity and a higher enzyme surface occupancy. The BsDyP-ERGO/ITO/PET electrode performed better than other horseradish peroxidase-based electrodes, as evinced by an improved electrochemical response in the nanomolar range (linearity 0.05-280 μM of H
O
, LOD 32 nM). The bioelectrode was mechanically robust, active in the 3.5-6 pH range and exhibited no loss of activity upon storage for 8 weeks at 4 °C. |
| doi_str_mv | 10.1002/cbic.202200346 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_cbic_202200346</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>35723909</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1079-50dc396285e5c191bbbd74cbddc46fa52ed5747a8d277d7fbe10418f21e2c1be3</originalsourceid><addsrcrecordid>eNo9kEtPwkAUhSdGI4huXZr5A8U7j3aYpSKCCQnGx7qZxy2MKS2ZFgP_3hKQxc25i--cxUfIPYMhA-CPzgY35MA5gJDZBekzKXSiMiEuT7_kXPXITdP8AIDOBLsmPZEqLjToPlk-G9diDKak7xjrXfCmQVpXdFKia2PtVrgOzpTlnn6g3zr0dBrNZoUV0kVHIy3qSGdhueqIT6ya0IZfpDPK6aK7F2y7mVBXt-SqMGWDd6cckO_Xydd4lswX07fx0zxxDJROUvBO6IyPUkwd08xa65V01nsns8KkHH2qpDIjz5XyqrDIQLJRwRlyxyyKARked12smyZikW9iWJu4zxnkB2P5wVh-NtYVHo6Fzdau0Z_xf0XiD9mgZ38</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Bhardwaj, Sheetal K ; Knaus, Tanja ; Garcia, Amanda ; Yan, Ning ; Mutti, Francesco G</creator><creatorcontrib>Bhardwaj, Sheetal K ; Knaus, Tanja ; Garcia, Amanda ; Yan, Ning ; Mutti, Francesco G</creatorcontrib><description>Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (ERGO) deposited on indium tin oxide (ITO) and polyethylene terephthalate (PET) layers. XRD, SEM, AFM, FT-IR and Raman characterization of the sensor confirmed its structural integrity and a higher enzyme surface occupancy. The BsDyP-ERGO/ITO/PET electrode performed better than other horseradish peroxidase-based electrodes, as evinced by an improved electrochemical response in the nanomolar range (linearity 0.05-280 μM of H
O
, LOD 32 nM). The bioelectrode was mechanically robust, active in the 3.5-6 pH range and exhibited no loss of activity upon storage for 8 weeks at 4 °C.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.202200346</identifier><identifier>PMID: 35723909</identifier><language>eng</language><publisher>Germany</publisher><subject>Biosensing Techniques ; Electrochemical Techniques ; Electrodes ; Graphite - chemistry ; Peroxidase ; Spectroscopy, Fourier Transform Infrared</subject><ispartof>Chembiochem : a European journal of chemical biology, 2022-09, Vol.23 (17), p.e202200346</ispartof><rights>2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1079-50dc396285e5c191bbbd74cbddc46fa52ed5747a8d277d7fbe10418f21e2c1be3</citedby><cites>FETCH-LOGICAL-c1079-50dc396285e5c191bbbd74cbddc46fa52ed5747a8d277d7fbe10418f21e2c1be3</cites><orcidid>0000-0002-6771-5102 ; 0000-0001-6677-7507 ; 0000-0001-9942-9226 ; 0000-0001-9876-7389</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35723909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhardwaj, Sheetal K</creatorcontrib><creatorcontrib>Knaus, Tanja</creatorcontrib><creatorcontrib>Garcia, Amanda</creatorcontrib><creatorcontrib>Yan, Ning</creatorcontrib><creatorcontrib>Mutti, Francesco G</creatorcontrib><title>Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection</title><title>Chembiochem : a European journal of chemical biology</title><addtitle>Chembiochem</addtitle><description>Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (ERGO) deposited on indium tin oxide (ITO) and polyethylene terephthalate (PET) layers. XRD, SEM, AFM, FT-IR and Raman characterization of the sensor confirmed its structural integrity and a higher enzyme surface occupancy. The BsDyP-ERGO/ITO/PET electrode performed better than other horseradish peroxidase-based electrodes, as evinced by an improved electrochemical response in the nanomolar range (linearity 0.05-280 μM of H
O
, LOD 32 nM). The bioelectrode was mechanically robust, active in the 3.5-6 pH range and exhibited no loss of activity upon storage for 8 weeks at 4 °C.</description><subject>Biosensing Techniques</subject><subject>Electrochemical Techniques</subject><subject>Electrodes</subject><subject>Graphite - chemistry</subject><subject>Peroxidase</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwkAUhSdGI4huXZr5A8U7j3aYpSKCCQnGx7qZxy2MKS2ZFgP_3hKQxc25i--cxUfIPYMhA-CPzgY35MA5gJDZBekzKXSiMiEuT7_kXPXITdP8AIDOBLsmPZEqLjToPlk-G9diDKak7xjrXfCmQVpXdFKia2PtVrgOzpTlnn6g3zr0dBrNZoUV0kVHIy3qSGdhueqIT6ya0IZfpDPK6aK7F2y7mVBXt-SqMGWDd6cckO_Xydd4lswX07fx0zxxDJROUvBO6IyPUkwd08xa65V01nsns8KkHH2qpDIjz5XyqrDIQLJRwRlyxyyKARked12smyZikW9iWJu4zxnkB2P5wVh-NtYVHo6Fzdau0Z_xf0XiD9mgZ38</recordid><startdate>20220905</startdate><enddate>20220905</enddate><creator>Bhardwaj, Sheetal K</creator><creator>Knaus, Tanja</creator><creator>Garcia, Amanda</creator><creator>Yan, Ning</creator><creator>Mutti, Francesco G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-6771-5102</orcidid><orcidid>https://orcid.org/0000-0001-6677-7507</orcidid><orcidid>https://orcid.org/0000-0001-9942-9226</orcidid><orcidid>https://orcid.org/0000-0001-9876-7389</orcidid></search><sort><creationdate>20220905</creationdate><title>Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection</title><author>Bhardwaj, Sheetal K ; Knaus, Tanja ; Garcia, Amanda ; Yan, Ning ; Mutti, Francesco G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1079-50dc396285e5c191bbbd74cbddc46fa52ed5747a8d277d7fbe10418f21e2c1be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biosensing Techniques</topic><topic>Electrochemical Techniques</topic><topic>Electrodes</topic><topic>Graphite - chemistry</topic><topic>Peroxidase</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhardwaj, Sheetal K</creatorcontrib><creatorcontrib>Knaus, Tanja</creatorcontrib><creatorcontrib>Garcia, Amanda</creatorcontrib><creatorcontrib>Yan, Ning</creatorcontrib><creatorcontrib>Mutti, Francesco G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Chembiochem : a European journal of chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhardwaj, Sheetal K</au><au>Knaus, Tanja</au><au>Garcia, Amanda</au><au>Yan, Ning</au><au>Mutti, Francesco G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection</atitle><jtitle>Chembiochem : a European journal of chemical biology</jtitle><addtitle>Chembiochem</addtitle><date>2022-09-05</date><risdate>2022</risdate><volume>23</volume><issue>17</issue><spage>e202200346</spage><pages>e202200346-</pages><issn>1439-4227</issn><eissn>1439-7633</eissn><abstract>Peroxidase enzymes enable the construction of electrochemical sensors for highly sensitive and selective quantitative detection of various molecules, pathogens and diseases. Herein, we describe the immobilization of a peroxidase from Bacillus s. (BsDyP) on electrochemically reduced graphene oxide (ERGO) deposited on indium tin oxide (ITO) and polyethylene terephthalate (PET) layers. XRD, SEM, AFM, FT-IR and Raman characterization of the sensor confirmed its structural integrity and a higher enzyme surface occupancy. The BsDyP-ERGO/ITO/PET electrode performed better than other horseradish peroxidase-based electrodes, as evinced by an improved electrochemical response in the nanomolar range (linearity 0.05-280 μM of H
O
, LOD 32 nM). The bioelectrode was mechanically robust, active in the 3.5-6 pH range and exhibited no loss of activity upon storage for 8 weeks at 4 °C.</abstract><cop>Germany</cop><pmid>35723909</pmid><doi>10.1002/cbic.202200346</doi><orcidid>https://orcid.org/0000-0002-6771-5102</orcidid><orcidid>https://orcid.org/0000-0001-6677-7507</orcidid><orcidid>https://orcid.org/0000-0001-9942-9226</orcidid><orcidid>https://orcid.org/0000-0001-9876-7389</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1439-4227 |
| ispartof | Chembiochem : a European journal of chemical biology, 2022-09, Vol.23 (17), p.e202200346 |
| issn | 1439-4227 1439-7633 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_cbic_202200346 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biosensing Techniques Electrochemical Techniques Electrodes Graphite - chemistry Peroxidase Spectroscopy, Fourier Transform Infrared |
| title | Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H 2 O 2 Detection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T22%3A09%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20Peroxidase%20on%20Electrochemically%20Reduced%20Graphene%20Oxide%20for%20Highly%20Sensitive%20H%202%20O%202%20Detection&rft.jtitle=Chembiochem%20:%20a%20European%20journal%20of%20chemical%20biology&rft.au=Bhardwaj,%20Sheetal%20K&rft.date=2022-09-05&rft.volume=23&rft.issue=17&rft.spage=e202200346&rft.pages=e202200346-&rft.issn=1439-4227&rft.eissn=1439-7633&rft_id=info:doi/10.1002/cbic.202200346&rft_dat=%3Cpubmed_cross%3E35723909%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/35723909&rfr_iscdi=true |