Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca 2+ independent Bacillus subtilis α-amylase

An extracellular amylase (AmyKS) produced by a newly isolated Bacillus subtilis strain US572 was purified and characterized. AmyKS showed maximal activity at pH 6 and 60°C with a half-life of 10 min at 70°C. It is a Ca independent enzyme and able to hydrolyze soluble starch into oligosaccharides con...

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Veröffentlicht in:	Biotechnology progress 2020-07, Vol.36 (4), p.e2964
Hauptverfasser:	Salem, Karima, Elgharbi, Fatma, Ben Hlima, Hajer, Perduca, Massimiliano, Sayari, Adel, Hmida-Sayari, Aïda
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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