Mechanism of pH-sensitive polymer-assisted protein refolding and its application in TGF-β1 and KGF-2

Refolding of proteins at high concentrations often results in non‐productive aggregation. This study, through a unique combination of spectroscopic and chromatographic analyzes, provides biomolecular evidence to demonstrate the ability of Eudragit S‐100, a pH‐responsive polymer, to enhance refolding...

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Veröffentlicht in:	Biotechnology progress 2009-09, Vol.25 (5), p.1387-1395
Hauptverfasser:	Huang, Zhifeng, Ni, Chunyan, Zhou, Xiangtian, Liu, Yanlong, Tan, Yi, Xiao, Jian, Feng, Wenke, Li, Xiaokun, Yang, Shulin
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Eudragit Fundamental and applied biological sciences. Psychology KGF-2 lysozyme protein refolding TGF-β1
Online-Zugang:	Volltext
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