Determination of ceftazidime in plasma by RP‐HPLC and ultraviolet detection

Determination of ceftazidime in plasma by RP‐HPLC and ultraviolet detection

A simple high‐performance liquid chromatography method for the determination of ceftazidime in plasma has been developed. Using an ultrafiltration technique samples were separated by reverse‐phase high‐performance liquid chromatography on a Symmetry C18 4.6 × 250 mm column (5.0 μm) and ultraviolet a...

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Veröffentlicht in:Biomedical chromatography 2021-07, Vol.35 (7), p.e5104-n/a
Hauptverfasser: Bergman, Joan, Harvill, Lainey, Hawkins, Shawna, Sladky, Kurt, Cox, Sherry
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ceftazidime
HPLC
pharmacokinetics
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container_issue 7
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container_title Biomedical chromatography
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creator Bergman, Joan
Harvill, Lainey
Hawkins, Shawna
Sladky, Kurt
Cox, Sherry
description A simple high‐performance liquid chromatography method for the determination of ceftazidime in plasma has been developed. Using an ultrafiltration technique samples were separated by reverse‐phase high‐performance liquid chromatography on a Symmetry C18 4.6 × 250 mm column (5.0 μm) and ultraviolet absorbance was measured at 260 nm. The mobile phase was a mixture of 10 mm potassium phosphate monobasic pH 2.5 with phosphoric acid and acetonitrile (90:10). The standard curve ranged from 0.1 to 100 μg/ml. Intra‐ and inter‐assay variability for ceftazidime was
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subjects ceftazidime
HPLC
pharmacokinetics
title Determination of ceftazidime in plasma by RP‐HPLC and ultraviolet detection
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