Preparation of immobilized invertase

Preparation of immobilized invertase

Immobilized invertase was prepared by ionically binding the enzyme to diethylaminoacetyl cellulose (DEAA‐cellulose). DEAA‐cellulose‐invertase complex was quite stable to electrolyte in the range of pH 5–7. Bound invertase was less active than the native enzyme, and approximately 55–70% of the enzyme...

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Veröffentlicht in:Biotechnology and bioengineering 1973-03, Vol.15 (2), p.403-412
Hauptverfasser: Maeda, Hidekatsu, Suzuki, Hideo, Sakimae, Akihiro
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container_title Biotechnology and bioengineering
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creator Maeda, Hidekatsu
Suzuki, Hideo
Sakimae, Akihiro
description Immobilized invertase was prepared by ionically binding the enzyme to diethylaminoacetyl cellulose (DEAA‐cellulose). DEAA‐cellulose‐invertase complex was quite stable to electrolyte in the range of pH 5–7. Bound invertase was less active than the native enzyme, and approximately 55–70% of the enzyme activity was lost on binding. The complex was stable for 9 days' continuous inversion in a column system at 30°C, but was rather unstable at 40°C. Heat stability and the effect of temperature on the reaction rate of the complex were almost identical with those of the native enzyme.
doi_str_mv 10.1002/bit.260150214
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