Decreased pCO 2 accumulation by eliminating bicarbonate addition to high cell‐density cultures

High‐density perfusion cultivation of mammalian cells can result in elevated bioreactor CO 2 partial pressure (pCO 2 ), a condition that can negatively influence growth, metabolism, productivity, and protein glycosylation. For BHK cells in a perfusion culture at 20 × 10 6 cells/mL, the bioreactor pC...

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Veröffentlicht in:	Biotechnology and bioengineering 2007-04, Vol.96 (6), p.1107-1117
Hauptverfasser:	Goudar, Chetan T., Matanguihan, Ricaredo, Long, Edward, Cruz, Christopher, Zhang, Chun, Piret, James M., Konstantinov, Konstantin B.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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