Enzyme electrodes for determination of total phenolic capacity of red wines

A tyrosinase‐modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4‐pyrrol‐1‐yl‐phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, Vmax, and Km, were determined. A...

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Veröffentlicht in:	Journal of applied polymer science 2005-10, Vol.98 (1), p.521-524
Hauptverfasser:	Kıralp, S., Cirpan, A., Toppare, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Biological and medical sciences Biotechnology conducting polymers copolymerization electrochemistry enzymes Exact sciences and technology Food industries Fundamental and applied biological sciences. Psychology General aspects Immobilization of enzymes and other molecules Immobilization techniques Methods of analysis, processing and quality control, regulation, standards Methods. Procedures. Technologies Physicochemistry of polymers Polymerization Polymers and radiations
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Beschreibung
Zusammenfassung:	A tyrosinase‐modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4‐pyrrol‐1‐yl‐phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, Vmax, and Km, were determined. Also, temperature, pH optimizations, operational stability and shelf life of the enzyme electrode were examined. Surface morphologies of the films were studied by scanning electron microscope. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 521–524, 2005
ISSN:	0021-8995 1097-4628
DOI:	10.1002/app.22165