Immobilization of glucose oxidase onto electrochemically prepared poly(aniline-co-fluoroaniline) films
Poly(aniline‐co‐fluoroaniline) [poly(An‐FAn)] films were electrochemically deposited on indium tin oxide glass plates. The characterization of these conducting copolymer films was carried out with ultraviolet–visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was imm...
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| Veröffentlicht in: | Journal of applied polymer science 2004-03, Vol.91 (6), p.3999-4006 |
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| container_title | Journal of applied polymer science |
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| creator | Sharma, Amit L. Singhal, Rahul Kumar, Arun Rajesh Pande, Krishan K. Malhotra, Bansi D. |
| description | Poly(aniline‐co‐fluoroaniline) [poly(An‐FAn)] films were electrochemically deposited on indium tin oxide glass plates. The characterization of these conducting copolymer films was carried out with ultraviolet–visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was immobilized onto the conducting poly(An‐FAn) films by a physical adsorption method. Amperometric response experiments carried out with poly(An‐FAn)/GOX films revealed linearity from 0.5 to 22 mM glucose, and the Michaelis–Menten constant was found to be about 23.5 mM. The shelf life of these poly(An‐FAn)/GOX electrodes was measured to be about 15 days, and the electrodes were stable up to 45°C. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3999–4006, 2004 |
| doi_str_mv | 10.1002/app.13553 |
| format | Article |
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The characterization of these conducting copolymer films was carried out with ultraviolet–visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was immobilized onto the conducting poly(An‐FAn) films by a physical adsorption method. Amperometric response experiments carried out with poly(An‐FAn)/GOX films revealed linearity from 0.5 to 22 mM glucose, and the Michaelis–Menten constant was found to be about 23.5 mM. The shelf life of these poly(An‐FAn)/GOX electrodes was measured to be about 15 days, and the electrodes were stable up to 45°C. © 2004 Wiley Periodicals, Inc. 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Appl. Polym. Sci</addtitle><description>Poly(aniline‐co‐fluoroaniline) [poly(An‐FAn)] films were electrochemically deposited on indium tin oxide glass plates. The characterization of these conducting copolymer films was carried out with ultraviolet–visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was immobilized onto the conducting poly(An‐FAn) films by a physical adsorption method. Amperometric response experiments carried out with poly(An‐FAn)/GOX films revealed linearity from 0.5 to 22 mM glucose, and the Michaelis–Menten constant was found to be about 23.5 mM. The shelf life of these poly(An‐FAn)/GOX electrodes was measured to be about 15 days, and the electrodes were stable up to 45°C. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3999–4006, 2004</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Immobilization of enzymes and other molecules</topic><topic>Immobilization techniques</topic><topic>Methods. Procedures. Technologies</topic><topic>Physicochemistry of polymers</topic><topic>Polymers and radiations</topic><topic>Properties and characterization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Amit L.</creatorcontrib><creatorcontrib>Singhal, Rahul</creatorcontrib><creatorcontrib>Kumar, Arun</creatorcontrib><creatorcontrib>Rajesh</creatorcontrib><creatorcontrib>Pande, Krishan K.</creatorcontrib><creatorcontrib>Malhotra, Bansi D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of applied polymer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Amit L.</au><au>Singhal, Rahul</au><au>Kumar, Arun</au><au>Rajesh</au><au>Pande, Krishan K.</au><au>Malhotra, Bansi D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of glucose oxidase onto electrochemically prepared poly(aniline-co-fluoroaniline) films</atitle><jtitle>Journal of applied polymer science</jtitle><addtitle>J. Appl. Polym. Sci</addtitle><date>2004-03-15</date><risdate>2004</risdate><volume>91</volume><issue>6</issue><spage>3999</spage><epage>4006</epage><pages>3999-4006</pages><issn>0021-8995</issn><eissn>1097-4628</eissn><coden>JAPNAB</coden><abstract>Poly(aniline‐co‐fluoroaniline) [poly(An‐FAn)] films were electrochemically deposited on indium tin oxide glass plates. The characterization of these conducting copolymer films was carried out with ultraviolet–visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was immobilized onto the conducting poly(An‐FAn) films by a physical adsorption method. Amperometric response experiments carried out with poly(An‐FAn)/GOX films revealed linearity from 0.5 to 22 mM glucose, and the Michaelis–Menten constant was found to be about 23.5 mM. The shelf life of these poly(An‐FAn)/GOX electrodes was measured to be about 15 days, and the electrodes were stable up to 45°C. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 91: 3999–4006, 2004</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><doi>10.1002/app.13553</doi><tpages>8</tpages></addata></record> |
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| subjects | Applied sciences Biological and medical sciences Biotechnology Exact sciences and technology Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies Physicochemistry of polymers Polymers and radiations Properties and characterization |
| title | Immobilization of glucose oxidase onto electrochemically prepared poly(aniline-co-fluoroaniline) films |
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