Controlled Supramolecular Self‐Assembly of Super‐charged β‐Lactoglobulin A–PEG Conjugates into Nanocapsules
The synthesis and characterization of a new protein–polymer conjugate composed of β lactoglobulin A (βLG A) and poly(ethylene glycol) PEG is described. βLG A was selectively modified to self‐assemble by super‐charging via amination or succinylation followed by conjugation with PEG. An equimolar mixt...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2017-09, Vol.56 (39), p.11754-11758 |
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| container_title | Angewandte Chemie International Edition |
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| creator | Khan, Amit Kumar Gudlur, Sushanth de Hoog, Hans‐Peter M. Siti, Winna Liedberg, Bo Nallani, Madhavan |
| description | The synthesis and characterization of a new protein–polymer conjugate composed of β lactoglobulin A (βLG A) and poly(ethylene glycol) PEG is described. βLG A was selectively modified to self‐assemble by super‐charging via amination or succinylation followed by conjugation with PEG. An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter. The self‐assembly proceeds by taking simultaneous advantage of the amphiphilicity and polyelectrolyte nature of the protein–polymer conjugate. These protein–polymer capsules or proteinosomes are reminiscent of protein capsids, and are capable of encapsulating solutes in their interior. We envisage this approach to be applicable to other globular proteins.
Super‐charged self‐assembly: The protein β lactoglobulin A (βLG A) was selectively modified to self‐assemble by amination or succinylation followed by conjugation with poly(ethylene glycol) (PEG). An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter. |
| doi_str_mv | 10.1002/anie.201704298 |
| format | Article |
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Super‐charged self‐assembly: The protein β lactoglobulin A (βLG A) was selectively modified to self‐assemble by amination or succinylation followed by conjugation with poly(ethylene glycol) (PEG). An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter.</description><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201704298</identifier><identifier>PMID: 28742233</identifier><language>eng</language><publisher>Germany</publisher><subject>nanostructures ; proteinosome ; protein–polymer conjugates ; selective protein modification ; self-assembly</subject><ispartof>Angewandte Chemie International Edition, 2017-09, Vol.56 (39), p.11754-11758</ispartof><rights>2017 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3458-f73a666c9cdb3507eddee1d7b187236241185161d80d83b623829a03cb39a4b23</citedby><cites>FETCH-LOGICAL-c3458-f73a666c9cdb3507eddee1d7b187236241185161d80d83b623829a03cb39a4b23</cites><orcidid>0000-0003-1143-6128</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201704298$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201704298$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28742233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Amit Kumar</creatorcontrib><creatorcontrib>Gudlur, Sushanth</creatorcontrib><creatorcontrib>de Hoog, Hans‐Peter M.</creatorcontrib><creatorcontrib>Siti, Winna</creatorcontrib><creatorcontrib>Liedberg, Bo</creatorcontrib><creatorcontrib>Nallani, Madhavan</creatorcontrib><title>Controlled Supramolecular Self‐Assembly of Super‐charged β‐Lactoglobulin A–PEG Conjugates into Nanocapsules</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>The synthesis and characterization of a new protein–polymer conjugate composed of β lactoglobulin A (βLG A) and poly(ethylene glycol) PEG is described. βLG A was selectively modified to self‐assemble by super‐charging via amination or succinylation followed by conjugation with PEG. An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter. The self‐assembly proceeds by taking simultaneous advantage of the amphiphilicity and polyelectrolyte nature of the protein–polymer conjugate. These protein–polymer capsules or proteinosomes are reminiscent of protein capsids, and are capable of encapsulating solutes in their interior. We envisage this approach to be applicable to other globular proteins.
Super‐charged self‐assembly: The protein β lactoglobulin A (βLG A) was selectively modified to self‐assemble by amination or succinylation followed by conjugation with poly(ethylene glycol) (PEG). An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter.</description><subject>nanostructures</subject><subject>proteinosome</subject><subject>protein–polymer conjugates</subject><subject>selective protein modification</subject><subject>self-assembly</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqFkEtOwzAURS0EoqUwZYiygRR_ktgZRlUplaqCVBhHtuOUVE4c2YlQZ10CEjthISyiK8FV-QwZPT_r3CO9C8A1gmMEIb7lTaXGGCIKI5yyEzBEMUYhoZSc-ndESEhZjAbgwrmN5xmDyTkYYEYjjAkZgm5ims4arVURrPrW8tpoJXvNbbBSutzv3jLnVC30NjDlgVDW_8kXbtc-8fnhlwWXnVlrI3pdNUG2370_TmeB9276Ne-UC6qmM8GSN0by1vVauUtwVnLt1NX3HIHnu-nT5D5cPMzmk2wRShLFLCwp4UmSyFQWgsSQqqJQChVUIEYxSXCEkL8tQQWDBSMiwYThlEMiBUl5JDAZgfHRK61xzqoyb21Vc7vNEcwP9eWH-vLf-nzg5hhoe1Gr4hf_6csD6RF4rbTa_qPLs-V8-if_AiWCgkc</recordid><startdate>20170918</startdate><enddate>20170918</enddate><creator>Khan, Amit Kumar</creator><creator>Gudlur, Sushanth</creator><creator>de Hoog, Hans‐Peter M.</creator><creator>Siti, Winna</creator><creator>Liedberg, Bo</creator><creator>Nallani, Madhavan</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-1143-6128</orcidid></search><sort><creationdate>20170918</creationdate><title>Controlled Supramolecular Self‐Assembly of Super‐charged β‐Lactoglobulin A–PEG Conjugates into Nanocapsules</title><author>Khan, Amit Kumar ; Gudlur, Sushanth ; de Hoog, Hans‐Peter M. ; Siti, Winna ; Liedberg, Bo ; Nallani, Madhavan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3458-f73a666c9cdb3507eddee1d7b187236241185161d80d83b623829a03cb39a4b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>nanostructures</topic><topic>proteinosome</topic><topic>protein–polymer conjugates</topic><topic>selective protein modification</topic><topic>self-assembly</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Amit Kumar</creatorcontrib><creatorcontrib>Gudlur, Sushanth</creatorcontrib><creatorcontrib>de Hoog, Hans‐Peter M.</creatorcontrib><creatorcontrib>Siti, Winna</creatorcontrib><creatorcontrib>Liedberg, Bo</creatorcontrib><creatorcontrib>Nallani, Madhavan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Amit Kumar</au><au>Gudlur, Sushanth</au><au>de Hoog, Hans‐Peter M.</au><au>Siti, Winna</au><au>Liedberg, Bo</au><au>Nallani, Madhavan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Controlled Supramolecular Self‐Assembly of Super‐charged β‐Lactoglobulin A–PEG Conjugates into Nanocapsules</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2017-09-18</date><risdate>2017</risdate><volume>56</volume><issue>39</issue><spage>11754</spage><epage>11758</epage><pages>11754-11758</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>The synthesis and characterization of a new protein–polymer conjugate composed of β lactoglobulin A (βLG A) and poly(ethylene glycol) PEG is described. βLG A was selectively modified to self‐assemble by super‐charging via amination or succinylation followed by conjugation with PEG. An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter. The self‐assembly proceeds by taking simultaneous advantage of the amphiphilicity and polyelectrolyte nature of the protein–polymer conjugate. These protein–polymer capsules or proteinosomes are reminiscent of protein capsids, and are capable of encapsulating solutes in their interior. We envisage this approach to be applicable to other globular proteins.
Super‐charged self‐assembly: The protein β lactoglobulin A (βLG A) was selectively modified to self‐assemble by amination or succinylation followed by conjugation with poly(ethylene glycol) (PEG). An equimolar mixture of the oppositely charged protein–polymer conjugates self‐assemble into spherical capsules of 80–100 nm in diameter.</abstract><cop>Germany</cop><pmid>28742233</pmid><doi>10.1002/anie.201704298</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0003-1143-6128</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | nanostructures proteinosome protein–polymer conjugates selective protein modification self-assembly |
| title | Controlled Supramolecular Self‐Assembly of Super‐charged β‐Lactoglobulin A–PEG Conjugates into Nanocapsules |
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