Curcumin inhibits CT26 cells metastasis by decreasing heparanase expression
This study tested the hypothesis that heparanase (HPSE) is related to tumor metastasis and curcumin (CCM) inhibits tumor metastasis by down‐regulating HPSE expression. MTT, Transwell assays, and RT‐PCR were used to study the effects of CCM on the migration and invasion of CT26 cells and the expressi...
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| Veröffentlicht in: | Journal of leukocyte biology 2020-12, Vol.108 (6), p.1727-1733 |
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| container_title | Journal of leukocyte biology |
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| creator | Li, Shanshan Fu, Hui Wang, Yiyang Wang, Li Jia, Beitian Bian, Yuhong |
| description | This study tested the hypothesis that heparanase (HPSE) is related to tumor metastasis and curcumin (CCM) inhibits tumor metastasis by down‐regulating HPSE expression. MTT, Transwell assays, and RT‐PCR were used to study the effects of CCM on the migration and invasion of CT26 cells and the expression of HPSE. CT26 cells were transfected with lentivirus to establish HPSE‐overexpressing cells (OE) and corresponding negative control cells (NC). Signal pathways involved in down‐regulating the expression of HPSE and inhibiting the migration and invasion of CT26 cells by CCM were screened by the liquid crystal chip. HPSE promoted CT26 cells migration and invasion, and CCM inhibited the proliferation and metastasis of CT26 cells. The results of RT‐PCR indicated that CCM down‐regulated HPSE expression. Liquid phase microarray showed that CCM inhibited the phosphorylation of P38 and STAT5 in CT26 cells and NC cells. In contrast, the inhibitory function of CCM was markedly enhanced when HPSE was overexpressed (P |
| doi_str_mv | 10.1002/JLB.1MA0620-357R |
| format | Article |
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Curcumin impacts CT26 cells migration and invasion by inhibiting HPSE expression.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1002/JLB.1MA0620-357R</identifier><identifier>PMID: 32640496</identifier><language>eng</language><publisher>United States</publisher><subject>Cell Line, Tumor ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - immunology ; Colonic Neoplasms - pathology ; Curcumin - pharmacology ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Enzymologic - immunology ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - immunology ; Glucuronidase - immunology ; Humans ; invasion ; JAK/STAT5 signal pathway ; MAP Kinase Signaling System - drug effects ; MAP Kinase Signaling System - immunology ; migration ; Neoplasm Metastasis ; Neoplasm Proteins - immunology ; P38 MAPK signal pathway ; traditional Chinese medicine</subject><ispartof>Journal of leukocyte biology, 2020-12, Vol.108 (6), p.1727-1733</ispartof><rights>2020 Society for Leukocyte Biology</rights><rights>2020 Society for Leukocyte Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2614-ef22bbcb6130253cc7b4baded9ebdf453fc6132273b4941c0acbaac097733a693</citedby><cites>FETCH-LOGICAL-c2614-ef22bbcb6130253cc7b4baded9ebdf453fc6132273b4941c0acbaac097733a693</cites><orcidid>0000-0001-9192-787X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2FJLB.1MA0620-357R$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2FJLB.1MA0620-357R$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32640496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Fu, Hui</creatorcontrib><creatorcontrib>Wang, Yiyang</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Jia, Beitian</creatorcontrib><creatorcontrib>Bian, Yuhong</creatorcontrib><title>Curcumin inhibits CT26 cells metastasis by decreasing heparanase expression</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>This study tested the hypothesis that heparanase (HPSE) is related to tumor metastasis and curcumin (CCM) inhibits tumor metastasis by down‐regulating HPSE expression. MTT, Transwell assays, and RT‐PCR were used to study the effects of CCM on the migration and invasion of CT26 cells and the expression of HPSE. CT26 cells were transfected with lentivirus to establish HPSE‐overexpressing cells (OE) and corresponding negative control cells (NC). Signal pathways involved in down‐regulating the expression of HPSE and inhibiting the migration and invasion of CT26 cells by CCM were screened by the liquid crystal chip. HPSE promoted CT26 cells migration and invasion, and CCM inhibited the proliferation and metastasis of CT26 cells. The results of RT‐PCR indicated that CCM down‐regulated HPSE expression. Liquid phase microarray showed that CCM inhibited the phosphorylation of P38 and STAT5 in CT26 cells and NC cells. In contrast, the inhibitory function of CCM was markedly enhanced when HPSE was overexpressed (P < 0.05). In short, HPSE is closely related to metastasis of colon cancer cells. CCM inhibits colon cancer cell migration and invasion by inhibiting HPSE expression, which may be related to P38 MAPK and JAK/STAT5 signal pathways.
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Curcumin impacts CT26 cells migration and invasion by inhibiting HPSE expression.</description><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - immunology</subject><subject>Colonic Neoplasms - pathology</subject><subject>Curcumin - pharmacology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Enzymologic - immunology</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - immunology</subject><subject>Glucuronidase - immunology</subject><subject>Humans</subject><subject>invasion</subject><subject>JAK/STAT5 signal pathway</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>MAP Kinase Signaling System - immunology</subject><subject>migration</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Proteins - immunology</subject><subject>P38 MAPK signal pathway</subject><subject>traditional Chinese medicine</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwzAQhC0EoqVw54T8B1LWj9jkWCLeRUionCPb2VCjJI1sKui_x1ULV6SVRrs7M4ePkHMGUwbALx_n11P2PAPFIRO5fj0gY1aIq0woLQ7JGLRkWS4BRuQkxg8AEFzBMRklkSALNSZP5Tq4ded76vult_4z0nLBFXXYtpF2-GliGh-p3dAaXcC09O90iYMJpjcRKX4PAWP0q_6UHDWmjXi21wl5u71ZlPfZ_OXuoZzNM8cVkxk2nFvrrGICeC6c01ZaU2NdoK0bmYvGpRfnWlhZSObAOGuMg0JrIYwqxITArteFVYwBm2oIvjNhUzGotlyqxKXac6m2XFLkYhcZ1rbD-i_wCyIZ1M7w5Vvc_Fu4PbCEV4ofV1xvSw</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>Li, Shanshan</creator><creator>Fu, Hui</creator><creator>Wang, Yiyang</creator><creator>Wang, Li</creator><creator>Jia, Beitian</creator><creator>Bian, Yuhong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-9192-787X</orcidid></search><sort><creationdate>202012</creationdate><title>Curcumin inhibits CT26 cells metastasis by decreasing heparanase expression</title><author>Li, Shanshan ; Fu, Hui ; Wang, Yiyang ; Wang, Li ; Jia, Beitian ; Bian, Yuhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2614-ef22bbcb6130253cc7b4baded9ebdf453fc6132273b4941c0acbaac097733a693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - immunology</topic><topic>Colonic Neoplasms - pathology</topic><topic>Curcumin - pharmacology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Enzymologic - immunology</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - immunology</topic><topic>Glucuronidase - immunology</topic><topic>Humans</topic><topic>invasion</topic><topic>JAK/STAT5 signal pathway</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>MAP Kinase Signaling System - immunology</topic><topic>migration</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Proteins - immunology</topic><topic>P38 MAPK signal pathway</topic><topic>traditional Chinese medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Fu, Hui</creatorcontrib><creatorcontrib>Wang, Yiyang</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Jia, Beitian</creatorcontrib><creatorcontrib>Bian, Yuhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shanshan</au><au>Fu, Hui</au><au>Wang, Yiyang</au><au>Wang, Li</au><au>Jia, Beitian</au><au>Bian, Yuhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin inhibits CT26 cells metastasis by decreasing heparanase expression</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2020-12</date><risdate>2020</risdate><volume>108</volume><issue>6</issue><spage>1727</spage><epage>1733</epage><pages>1727-1733</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>This study tested the hypothesis that heparanase (HPSE) is related to tumor metastasis and curcumin (CCM) inhibits tumor metastasis by down‐regulating HPSE expression. MTT, Transwell assays, and RT‐PCR were used to study the effects of CCM on the migration and invasion of CT26 cells and the expression of HPSE. CT26 cells were transfected with lentivirus to establish HPSE‐overexpressing cells (OE) and corresponding negative control cells (NC). Signal pathways involved in down‐regulating the expression of HPSE and inhibiting the migration and invasion of CT26 cells by CCM were screened by the liquid crystal chip. HPSE promoted CT26 cells migration and invasion, and CCM inhibited the proliferation and metastasis of CT26 cells. The results of RT‐PCR indicated that CCM down‐regulated HPSE expression. Liquid phase microarray showed that CCM inhibited the phosphorylation of P38 and STAT5 in CT26 cells and NC cells. In contrast, the inhibitory function of CCM was markedly enhanced when HPSE was overexpressed (P < 0.05). In short, HPSE is closely related to metastasis of colon cancer cells. CCM inhibits colon cancer cell migration and invasion by inhibiting HPSE expression, which may be related to P38 MAPK and JAK/STAT5 signal pathways.
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Curcumin impacts CT26 cells migration and invasion by inhibiting HPSE expression.</abstract><cop>United States</cop><pmid>32640496</pmid><doi>10.1002/JLB.1MA0620-357R</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-9192-787X</orcidid></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Cell Line, Tumor Colonic Neoplasms - drug therapy Colonic Neoplasms - immunology Colonic Neoplasms - pathology Curcumin - pharmacology Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Enzymologic - immunology Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - immunology Glucuronidase - immunology Humans invasion JAK/STAT5 signal pathway MAP Kinase Signaling System - drug effects MAP Kinase Signaling System - immunology migration Neoplasm Metastasis Neoplasm Proteins - immunology P38 MAPK signal pathway traditional Chinese medicine |
| title | Curcumin inhibits CT26 cells metastasis by decreasing heparanase expression |
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