Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell‐based therapies for a number of diseases including Park...
Gespeichert in:
| Veröffentlicht in: | Current Protocols in Stem Cell Biology 2007-06, Vol.1 (1), p.1A.1.1-1A.1.21 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1A.1.21 |
|---|---|
| container_issue | 1 |
| container_start_page | 1A.1.1 |
| container_title | Current Protocols in Stem Cell Biology |
| container_volume | 1 |
| creator | Navara, Christopher S. Redinger, Carrie Mich‐Basso, Jocelyn Oliver, Stacie Ben‐Yehudah, Ahmi Castro, Carlos Simerly, Calvin |
| description | Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell‐based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell–derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype. Curr. Protoc. Stem Cell Biol. 1:1A.1.1‐1A.1.21. © 2007 by John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/9780470151808.sc01a01s1 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_9780470151808_sc01a01s1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CPSC01A01</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2811-4c754ad2f4c460993668d90ecfb5a1aaac7858aeb2c4e2c9e8bf387d263d4b13</originalsourceid><addsrcrecordid>eNqNkF9LwzAUxYMobsx9Bc0X6Lw3SZvkRRh1c8LQwfZe0jRlhf4ZTafMT29Hh-KbT_dyOOdw-BHygDBDAPaopQIhAUNUoGbeAhpAj1dkjJqrQOlIX59_gYHkjI3I1PsiBeAIQmh1S0aopAoxlGOyenZt8WG6oqmpqTMa701rbNeLX4PY5PStqffHytR00xaV6RxdVGl7aurC0m3nKhq7svR35CY3pXfTy52Q3XKxi1fB-v3lNZ6vA8sUYiCsDIXJWC6siEBrHkUq0-BsnoYGjTG2X6aMS5kVjlntVJpzJTMW8UykyCdEDrW2bbxvXZ4czqPaU4KQnOkkf-gkP3T65P2QPBzTymW_uQuL3vA0GD6L0p3-25vEm20MOAfk37e-c-M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Navara, Christopher S. ; Redinger, Carrie ; Mich‐Basso, Jocelyn ; Oliver, Stacie ; Ben‐Yehudah, Ahmi ; Castro, Carlos ; Simerly, Calvin</creator><creatorcontrib>Navara, Christopher S. ; Redinger, Carrie ; Mich‐Basso, Jocelyn ; Oliver, Stacie ; Ben‐Yehudah, Ahmi ; Castro, Carlos ; Simerly, Calvin</creatorcontrib><description>Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell‐based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell–derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype. Curr. Protoc. Stem Cell Biol. 1:1A.1.1‐1A.1.21. © 2007 by John Wiley & Sons, Inc.</description><identifier>ISSN: 1941-7322</identifier><identifier>EISSN: 1938-8969</identifier><identifier>DOI: 10.1002/9780470151808.sc01a01s1</identifier><identifier>PMID: 18785157</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Base Sequence ; Cell Culture Techniques ; Cell Separation ; DNA Primers - genetics ; embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Embryonic Stem Cells - transplantation ; Gene Expression ; Immunohistochemistry ; Karyotype ; Karyotyping ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Nanog ; nonhuman primate ; Oct‐4 ; Phenotype ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Pluripotent Stem Cells - transplantation ; Primates - embryology ; Primates - genetics ; Primates - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Teratoma ; Teratoma - etiology ; Transplantation, Heterologous</subject><ispartof>Current Protocols in Stem Cell Biology, 2007-06, Vol.1 (1), p.1A.1.1-1A.1.21</ispartof><rights>Copyright © 2007 John Wiley & Sons, Inc.</rights><rights>Copyright 2007 by John Wiley & Sons, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2811-4c754ad2f4c460993668d90ecfb5a1aaac7858aeb2c4e2c9e8bf387d263d4b13</citedby><cites>FETCH-LOGICAL-c2811-4c754ad2f4c460993668d90ecfb5a1aaac7858aeb2c4e2c9e8bf387d263d4b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18785157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Navara, Christopher S.</creatorcontrib><creatorcontrib>Redinger, Carrie</creatorcontrib><creatorcontrib>Mich‐Basso, Jocelyn</creatorcontrib><creatorcontrib>Oliver, Stacie</creatorcontrib><creatorcontrib>Ben‐Yehudah, Ahmi</creatorcontrib><creatorcontrib>Castro, Carlos</creatorcontrib><creatorcontrib>Simerly, Calvin</creatorcontrib><title>Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells</title><title>Current Protocols in Stem Cell Biology</title><addtitle>Curr Protoc Stem Cell Biol</addtitle><description>Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell‐based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell–derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype. Curr. Protoc. Stem Cell Biol. 1:1A.1.1‐1A.1.21. © 2007 by John Wiley & Sons, Inc.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Culture Techniques</subject><subject>Cell Separation</subject><subject>DNA Primers - genetics</subject><subject>embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Embryonic Stem Cells - transplantation</subject><subject>Gene Expression</subject><subject>Immunohistochemistry</subject><subject>Karyotype</subject><subject>Karyotyping</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Nanog</subject><subject>nonhuman primate</subject><subject>Oct‐4</subject><subject>Phenotype</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Pluripotent Stem Cells - transplantation</subject><subject>Primates - embryology</subject><subject>Primates - genetics</subject><subject>Primates - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Teratoma</subject><subject>Teratoma - etiology</subject><subject>Transplantation, Heterologous</subject><issn>1941-7322</issn><issn>1938-8969</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF9LwzAUxYMobsx9Bc0X6Lw3SZvkRRh1c8LQwfZe0jRlhf4ZTafMT29Hh-KbT_dyOOdw-BHygDBDAPaopQIhAUNUoGbeAhpAj1dkjJqrQOlIX59_gYHkjI3I1PsiBeAIQmh1S0aopAoxlGOyenZt8WG6oqmpqTMa701rbNeLX4PY5PStqffHytR00xaV6RxdVGl7aurC0m3nKhq7svR35CY3pXfTy52Q3XKxi1fB-v3lNZ6vA8sUYiCsDIXJWC6siEBrHkUq0-BsnoYGjTG2X6aMS5kVjlntVJpzJTMW8UykyCdEDrW2bbxvXZ4czqPaU4KQnOkkf-gkP3T65P2QPBzTymW_uQuL3vA0GD6L0p3-25vEm20MOAfk37e-c-M</recordid><startdate>200706</startdate><enddate>200706</enddate><creator>Navara, Christopher S.</creator><creator>Redinger, Carrie</creator><creator>Mich‐Basso, Jocelyn</creator><creator>Oliver, Stacie</creator><creator>Ben‐Yehudah, Ahmi</creator><creator>Castro, Carlos</creator><creator>Simerly, Calvin</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200706</creationdate><title>Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells</title><author>Navara, Christopher S. ; Redinger, Carrie ; Mich‐Basso, Jocelyn ; Oliver, Stacie ; Ben‐Yehudah, Ahmi ; Castro, Carlos ; Simerly, Calvin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2811-4c754ad2f4c460993668d90ecfb5a1aaac7858aeb2c4e2c9e8bf387d263d4b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Culture Techniques</topic><topic>Cell Separation</topic><topic>DNA Primers - genetics</topic><topic>embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Embryonic Stem Cells - transplantation</topic><topic>Gene Expression</topic><topic>Immunohistochemistry</topic><topic>Karyotype</topic><topic>Karyotyping</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Nanog</topic><topic>nonhuman primate</topic><topic>Oct‐4</topic><topic>Phenotype</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Pluripotent Stem Cells - transplantation</topic><topic>Primates - embryology</topic><topic>Primates - genetics</topic><topic>Primates - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Teratoma</topic><topic>Teratoma - etiology</topic><topic>Transplantation, Heterologous</topic><toplevel>online_resources</toplevel><creatorcontrib>Navara, Christopher S.</creatorcontrib><creatorcontrib>Redinger, Carrie</creatorcontrib><creatorcontrib>Mich‐Basso, Jocelyn</creatorcontrib><creatorcontrib>Oliver, Stacie</creatorcontrib><creatorcontrib>Ben‐Yehudah, Ahmi</creatorcontrib><creatorcontrib>Castro, Carlos</creatorcontrib><creatorcontrib>Simerly, Calvin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Current Protocols in Stem Cell Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Navara, Christopher S.</au><au>Redinger, Carrie</au><au>Mich‐Basso, Jocelyn</au><au>Oliver, Stacie</au><au>Ben‐Yehudah, Ahmi</au><au>Castro, Carlos</au><au>Simerly, Calvin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells</atitle><jtitle>Current Protocols in Stem Cell Biology</jtitle><addtitle>Curr Protoc Stem Cell Biol</addtitle><date>2007-06</date><risdate>2007</risdate><volume>1</volume><issue>1</issue><spage>1A.1.1</spage><epage>1A.1.21</epage><pages>1A.1.1-1A.1.21</pages><issn>1941-7322</issn><eissn>1938-8969</eissn><abstract>Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell‐based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell–derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype. Curr. Protoc. Stem Cell Biol. 1:1A.1.1‐1A.1.21. © 2007 by John Wiley & Sons, Inc.</abstract><cop>United States</cop><pmid>18785157</pmid><doi>10.1002/9780470151808.sc01a01s1</doi><tpages>21</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1941-7322 |
| ispartof | Current Protocols in Stem Cell Biology, 2007-06, Vol.1 (1), p.1A.1.1-1A.1.21 |
| issn | 1941-7322 1938-8969 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_9780470151808_sc01a01s1 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Cell Culture Techniques Cell Separation DNA Primers - genetics embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryonic Stem Cells - transplantation Gene Expression Immunohistochemistry Karyotype Karyotyping Mice Mice, Inbred NOD Mice, SCID Nanog nonhuman primate Oct‐4 Phenotype Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Pluripotent Stem Cells - transplantation Primates - embryology Primates - genetics Primates - metabolism Reverse Transcriptase Polymerase Chain Reaction Teratoma Teratoma - etiology Transplantation, Heterologous |
| title | Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T22%3A59%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Derivation%20and%20Characterization%20of%20Nonhuman%20Primate%20Embryonic%20Stem%20Cells&rft.jtitle=Current%20Protocols%20in%20Stem%20Cell%20Biology&rft.au=Navara,%20Christopher%20S.&rft.date=2007-06&rft.volume=1&rft.issue=1&rft.spage=1A.1.1&rft.epage=1A.1.21&rft.pages=1A.1.1-1A.1.21&rft.issn=1941-7322&rft.eissn=1938-8969&rft_id=info:doi/10.1002/9780470151808.sc01a01s1&rft_dat=%3Cwiley_cross%3ECPSC01A01%3C/wiley_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/18785157&rfr_iscdi=true |