Measurement of T Cell Activation After 16‐hr In Vitro Stimulation with Concanavalin A

A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here. Human whole blood is incubated with fluorescently labeled antibodies (against CD3, CD4, CD8, and CD69), erythr...

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Veröffentlicht in:	Current Protocols in Cytometry 2010-01, Vol.51 (1), p.6.28.1-6.28.10
1. Verfasser:	Arneth, Borros M.
Format:	Artikel
Sprache:	eng
Schlagworte:	activated T cells activation marker Antibodies, Monoclonal - pharmacology CD4 T cells CD4 T helper cells CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - immunology CD69 CD69 activation marker CD8 cytotoxic T cells CD8-Positive T-Lymphocytes - drug effects CD8-Positive T-Lymphocytes - immunology Cell Extracts Concanavalin A - immunology Concanavalin A - pharmacology Erythrocytes - drug effects Flow Cytometry - methods Humans Immunophenotyping Lymphocyte Activation - drug effects lymphocyte proliferation assay Lymphocyte Subsets - drug effects lymphocytes T lymphocytes Time Factors
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