Light Scatter: Detection and Usage
Light scatter is one of the most basic of measurements made in flow cytometry. This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a...
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Veröffentlicht in: | Current Protocols in Cytometry 2001-05, Vol.9 (1), p.1.13.1-1.13.8 |
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container_title | Current Protocols in Cytometry |
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creator | Salzman, Gary C. |
description | Light scatter is one of the most basic of measurements made in flow cytometry. This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a discussion of methods for detecting scattered light in a flow cytometer. The unit ends with a brief survey showing how light scattering is used in a broad range of flow cytometry applications. |
doi_str_mv | 10.1002/0471142956.cy0113s09 |
format | Article |
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This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a discussion of methods for detecting scattered light in a flow cytometer. The unit ends with a brief survey showing how light scattering is used in a broad range of flow cytometry applications.</description><identifier>ISSN: 1934-9297</identifier><identifier>EISSN: 1934-9300</identifier><identifier>DOI: 10.1002/0471142956.cy0113s09</identifier><identifier>PMID: 18770663</identifier><language>eng</language><publisher>United States</publisher><subject>Calibration ; Cell Separation - instrumentation ; Cell Separation - methods ; Equipment Design ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Granulocytes - cytology ; Humans ; Leukocytes - cytology ; Light ; Scattering, Radiation</subject><ispartof>Current Protocols in Cytometry, 2001-05, Vol.9 (1), p.1.13.1-1.13.8</ispartof><rights>1999 John Wiley & Sons, Inc. 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identifier | ISSN: 1934-9297 |
ispartof | Current Protocols in Cytometry, 2001-05, Vol.9 (1), p.1.13.1-1.13.8 |
issn | 1934-9297 1934-9300 |
language | eng |
recordid | cdi_crossref_primary_10_1002_0471142956_cy0113s09 |
source | MEDLINE; Alma/SFX Local Collection |
subjects | Calibration Cell Separation - instrumentation Cell Separation - methods Equipment Design Flow Cytometry - instrumentation Flow Cytometry - methods Granulocytes - cytology Humans Leukocytes - cytology Light Scattering, Radiation |
title | Light Scatter: Detection and Usage |
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