Light Scatter: Detection and Usage

Light Scatter: Detection and Usage

Light scatter is one of the most basic of measurements made in flow cytometry. This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a...

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Veröffentlicht in:Current Protocols in Cytometry 2001-05, Vol.9 (1), p.1.13.1-1.13.8
1. Verfasser: Salzman, Gary C.
Format: Artikel
Sprache:eng
Schlagworte:
Calibration
Cell Separation - instrumentation
Cell Separation - methods
Equipment Design
Flow Cytometry - instrumentation
Flow Cytometry - methods
Granulocytes - cytology
Humans
Leukocytes - cytology
Light
Scattering, Radiation
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Beschreibung
Zusammenfassung:Light scatter is one of the most basic of measurements made in flow cytometry. This unit presents a short commentary on what light scatter is and how it is measured. First, the author describes the basic principles of the scattering of light from small particles such as cells. This is followed by a discussion of methods for detecting scattered light in a flow cytometer. The unit ends with a brief survey showing how light scattering is used in a broad range of flow cytometry applications.
ISSN:1934-9297
1934-9300
DOI:10.1002/0471142956.cy0113s09