Production of Recombinant Adeno‐Associated Viral Vectors
Recombinant adeno‐associated viral (rAAV) vectors are capable of delivering genes to both dividing and nondividing cells. This property, along with the physical stability of the virions, permits transduction of cells both in tissue culture and in the whole animal. In this unit, protocols for the ade...
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| Veröffentlicht in: | Current Protocols in Human Genetics 1999-11, Vol.23 (1), p.12.9.1-12.9.17 |
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| container_end_page | 12.9.17 |
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| container_issue | 1 |
| container_start_page | 12.9.1 |
| container_title | Current Protocols in Human Genetics |
| container_volume | 23 |
| creator | Haberman, Rebecca A. Kroner‐Lux, Gabriele Samulski, Richard Jude |
| description | Recombinant adeno‐associated viral (rAAV) vectors are capable of delivering genes to both dividing and nondividing cells. This property, along with the physical stability of the virions, permits transduction of cells both in tissue culture and in the whole animal. In this unit, protocols for the adenovirus‐free production of rAAV vectors, their purification, and their quantitation are provided. The production and purification of the rAAV vectors are described, using two different methods. Two assays for determining rAAV titers are also presented: dot blot and in vitro infection with a transgene expression assay. |
| doi_str_mv | 10.1002/0471142905.hg1209s23 |
| format | Article |
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| identifier | ISSN: 1934-8266 |
| ispartof | Current Protocols in Human Genetics, 1999-11, Vol.23 (1), p.12.9.1-12.9.17 |
| issn | 1934-8266 1934-8258 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_0471142905_hg1209s23 |
| source | Alma/SFX Local Collection |
| title | Production of Recombinant Adeno‐Associated Viral Vectors |
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