TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue
Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas proje...
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| Veröffentlicht in: | CANCERS 2021-01, Vol.13 (1), p.114 |
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| creator | Sjöstedt, Evelina Kolnes, Anders J Olarescu, Nicoleta C Mitsios, Nicholas Hikmet, Feria Sivertsson, Åsa Lindskog, Cecilia Øystese, Kristin A B Jørgensen, Anders P Bollerslev, Jens Casar-Borota, Olivera |
| description | Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas project (HPA074356) was used for TGFBR3L staining and combined with SF1 and FSH for a 3-plex fluorescent protocol, providing more details about the cell lineage specificity of TGFBR3L expression. A cohort of 230 pituitary neuroendocrine tumours were analysed. In a subgroup of previously characterised gonadotroph tumours, correlation with expression of FSH/LH, E-cadherin, oestrogen (ER) and somatostatin receptors (SSTR) was explored. TGFBR3L showed membranous immunolabeling and was found to be gonadotroph cell lineage-specific, verified by co-expression with SF1 and FSH/LH staining in both tumour and non-neoplastic anterior pituitary tissues. TGFBR3L immunoreactivity was observed in gonadotroph tumours only and demonstrated intra-tumour heterogeneity with a perivascular location. TGFBR3L immunostaining correlated positively to both FSH (
= 0.290) and LH (
= 0.390) immunostaining, and SSTR3 (
= 0.315). TGFBR3L correlated inversely to membranous E-cadherin staining (
= -0.351) and oestrogen receptor β mRNA (
= -0.274). In conclusion, TGFBR3L is a novel pituitary gland specific protein, located in the membrane of gonadotroph cells in non-neoplastic anterior pituitary gland and in a subset of gonadotroph pituitary tumours. |
| doi_str_mv | 10.3390/cancers13010114 |
| format | Article |
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= 0.290) and LH (
= 0.390) immunostaining, and SSTR3 (
= 0.315). TGFBR3L correlated inversely to membranous E-cadherin staining (
= -0.351) and oestrogen receptor β mRNA (
= -0.274). In conclusion, TGFBR3L is a novel pituitary gland specific protein, located in the membrane of gonadotroph cells in non-neoplastic anterior pituitary gland and in a subset of gonadotroph pituitary tumours.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers13010114</identifier><identifier>PMID: 33396509</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Brain tumors ; Cell lineage ; E-cadherin ; Follicle-stimulating hormone ; Genes ; gonadotroph cells ; Growth factors ; hormone secretion ; Hormones ; immunohistochemistry ; Immunoreactivity ; Luteinizing hormone ; membrane protein ; Membrane proteins ; mRNA ; Neuroendocrine tumors ; Pathology ; Patologi ; Pituitary (anterior) ; Pituitary gland ; pituitary neuroendocrine tumours ; Polyclonal antibodies ; Proteins ; Somatostatin ; Somatostatin receptors ; Transcription factors ; Tumors</subject><ispartof>CANCERS, 2021-01, Vol.13 (1), p.114</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>info:eu-repo/semantics/openAccess</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-8b1eac058bffffbb87f20f11458fa3e2fb7a98680f0e985835415524e21755303</citedby><cites>FETCH-LOGICAL-c558t-8b1eac058bffffbb87f20f11458fa3e2fb7a98680f0e985835415524e21755303</cites><orcidid>0000-0002-0327-7377 ; 0000-0003-4607-9642 ; 0000-0001-7376-7331 ; 0000-0002-1246-9194 ; 0000-0002-3147-6007</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795056/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795056/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,550,723,776,780,881,26544,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33396509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-289514$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-431278$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:145592710$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Sjöstedt, Evelina</creatorcontrib><creatorcontrib>Kolnes, Anders J</creatorcontrib><creatorcontrib>Olarescu, Nicoleta C</creatorcontrib><creatorcontrib>Mitsios, Nicholas</creatorcontrib><creatorcontrib>Hikmet, Feria</creatorcontrib><creatorcontrib>Sivertsson, Åsa</creatorcontrib><creatorcontrib>Lindskog, Cecilia</creatorcontrib><creatorcontrib>Øystese, Kristin A B</creatorcontrib><creatorcontrib>Jørgensen, Anders P</creatorcontrib><creatorcontrib>Bollerslev, Jens</creatorcontrib><creatorcontrib>Casar-Borota, Olivera</creatorcontrib><title>TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue</title><title>CANCERS</title><addtitle>Cancers (Basel)</addtitle><description>Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas project (HPA074356) was used for TGFBR3L staining and combined with SF1 and FSH for a 3-plex fluorescent protocol, providing more details about the cell lineage specificity of TGFBR3L expression. A cohort of 230 pituitary neuroendocrine tumours were analysed. In a subgroup of previously characterised gonadotroph tumours, correlation with expression of FSH/LH, E-cadherin, oestrogen (ER) and somatostatin receptors (SSTR) was explored. TGFBR3L showed membranous immunolabeling and was found to be gonadotroph cell lineage-specific, verified by co-expression with SF1 and FSH/LH staining in both tumour and non-neoplastic anterior pituitary tissues. TGFBR3L immunoreactivity was observed in gonadotroph tumours only and demonstrated intra-tumour heterogeneity with a perivascular location. TGFBR3L immunostaining correlated positively to both FSH (
= 0.290) and LH (
= 0.390) immunostaining, and SSTR3 (
= 0.315). TGFBR3L correlated inversely to membranous E-cadherin staining (
= -0.351) and oestrogen receptor β mRNA (
= -0.274). In conclusion, TGFBR3L is a novel pituitary gland specific protein, located in the membrane of gonadotroph cells in non-neoplastic anterior pituitary gland and in a subset of gonadotroph pituitary tumours.</description><subject>Brain tumors</subject><subject>Cell lineage</subject><subject>E-cadherin</subject><subject>Follicle-stimulating hormone</subject><subject>Genes</subject><subject>gonadotroph cells</subject><subject>Growth factors</subject><subject>hormone secretion</subject><subject>Hormones</subject><subject>immunohistochemistry</subject><subject>Immunoreactivity</subject><subject>Luteinizing hormone</subject><subject>membrane protein</subject><subject>Membrane proteins</subject><subject>mRNA</subject><subject>Neuroendocrine tumors</subject><subject>Pathology</subject><subject>Patologi</subject><subject>Pituitary (anterior)</subject><subject>Pituitary gland</subject><subject>pituitary neuroendocrine tumours</subject><subject>Polyclonal 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Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue</title><author>Sjöstedt, Evelina ; Kolnes, Anders J ; Olarescu, Nicoleta C ; Mitsios, Nicholas ; Hikmet, Feria ; Sivertsson, Åsa ; Lindskog, Cecilia ; Øystese, Kristin A B ; Jørgensen, Anders P ; Bollerslev, Jens ; Casar-Borota, Olivera</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-8b1eac058bffffbb87f20f11458fa3e2fb7a98680f0e985835415524e21755303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Brain tumors</topic><topic>Cell lineage</topic><topic>E-cadherin</topic><topic>Follicle-stimulating hormone</topic><topic>Genes</topic><topic>gonadotroph cells</topic><topic>Growth factors</topic><topic>hormone secretion</topic><topic>Hormones</topic><topic>immunohistochemistry</topic><topic>Immunoreactivity</topic><topic>Luteinizing hormone</topic><topic>membrane protein</topic><topic>Membrane proteins</topic><topic>mRNA</topic><topic>Neuroendocrine tumors</topic><topic>Pathology</topic><topic>Patologi</topic><topic>Pituitary (anterior)</topic><topic>Pituitary gland</topic><topic>pituitary neuroendocrine tumours</topic><topic>Polyclonal antibodies</topic><topic>Proteins</topic><topic>Somatostatin</topic><topic>Somatostatin receptors</topic><topic>Transcription factors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sjöstedt, Evelina</creatorcontrib><creatorcontrib>Kolnes, Anders J</creatorcontrib><creatorcontrib>Olarescu, Nicoleta C</creatorcontrib><creatorcontrib>Mitsios, Nicholas</creatorcontrib><creatorcontrib>Hikmet, Feria</creatorcontrib><creatorcontrib>Sivertsson, Åsa</creatorcontrib><creatorcontrib>Lindskog, Cecilia</creatorcontrib><creatorcontrib>Øystese, Kristin A B</creatorcontrib><creatorcontrib>Jørgensen, Anders 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Academic</collection><collection>NORA - Norwegian Open Research Archives</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Kungliga Tekniska Högskolan</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><jtitle>CANCERS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sjöstedt, Evelina</au><au>Kolnes, Anders J</au><au>Olarescu, Nicoleta C</au><au>Mitsios, Nicholas</au><au>Hikmet, Feria</au><au>Sivertsson, Åsa</au><au>Lindskog, Cecilia</au><au>Øystese, Kristin A B</au><au>Jørgensen, Anders P</au><au>Bollerslev, Jens</au><au>Casar-Borota, Olivera</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue</atitle><jtitle>CANCERS</jtitle><addtitle>Cancers (Basel)</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>13</volume><issue>1</issue><spage>114</spage><pages>114-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas project (HPA074356) was used for TGFBR3L staining and combined with SF1 and FSH for a 3-plex fluorescent protocol, providing more details about the cell lineage specificity of TGFBR3L expression. A cohort of 230 pituitary neuroendocrine tumours were analysed. In a subgroup of previously characterised gonadotroph tumours, correlation with expression of FSH/LH, E-cadherin, oestrogen (ER) and somatostatin receptors (SSTR) was explored. TGFBR3L showed membranous immunolabeling and was found to be gonadotroph cell lineage-specific, verified by co-expression with SF1 and FSH/LH staining in both tumour and non-neoplastic anterior pituitary tissues. TGFBR3L immunoreactivity was observed in gonadotroph tumours only and demonstrated intra-tumour heterogeneity with a perivascular location. TGFBR3L immunostaining correlated positively to both FSH (
= 0.290) and LH (
= 0.390) immunostaining, and SSTR3 (
= 0.315). TGFBR3L correlated inversely to membranous E-cadherin staining (
= -0.351) and oestrogen receptor β mRNA (
= -0.274). In conclusion, TGFBR3L is a novel pituitary gland specific protein, located in the membrane of gonadotroph cells in non-neoplastic anterior pituitary gland and in a subset of gonadotroph pituitary tumours.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33396509</pmid><doi>10.3390/cancers13010114</doi><orcidid>https://orcid.org/0000-0002-0327-7377</orcidid><orcidid>https://orcid.org/0000-0003-4607-9642</orcidid><orcidid>https://orcid.org/0000-0001-7376-7331</orcidid><orcidid>https://orcid.org/0000-0002-1246-9194</orcidid><orcidid>https://orcid.org/0000-0002-3147-6007</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2072-6694 |
| ispartof | CANCERS, 2021-01, Vol.13 (1), p.114 |
| issn | 2072-6694 2072-6694 |
| language | eng |
| recordid | cdi_cristin_nora_10852_86286 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; NORA - Norwegian Open Research Archives; PubMed Central; SWEPUB Freely available online; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Brain tumors Cell lineage E-cadherin Follicle-stimulating hormone Genes gonadotroph cells Growth factors hormone secretion Hormones immunohistochemistry Immunoreactivity Luteinizing hormone membrane protein Membrane proteins mRNA Neuroendocrine tumors Pathology Patologi Pituitary (anterior) Pituitary gland pituitary neuroendocrine tumours Polyclonal antibodies Proteins Somatostatin Somatostatin receptors Transcription factors Tumors |
| title | TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue |
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