Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients
Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the...
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| Veröffentlicht in: | Biofouling (Chur, Switzerland) Switzerland), 2019-02, Vol.35 (2), p.204-216 |
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| creator | Ikram, Samman Heikal, Adam Finke, Sarah Hofgaard, Antje Rehman, Yasir Sabri, Anjum Nasim Økstad, Ole Andreas |
| description | Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal β-lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs. |
| doi_str_mv | 10.1080/08927014.2019.1586889 |
| format | Article |
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Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal β-lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs.</description><identifier>ISSN: 0892-7014</identifier><identifier>EISSN: 1029-2454</identifier><identifier>DOI: 10.1080/08927014.2019.1586889</identifier><identifier>PMID: 30950292</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Ampicillin ; Bacillus cereus ; Bacillus cereus group ; Bacteria ; Biofilm ; Biofilms ; cardiac patients ; Catheters ; central venous catheters ; Chromosomes ; Clindamycin ; comparative genomics ; Contamination ; DNA ; Gene sequencing ; Genes ; Genomes ; Heart ; Immunocompromised hosts ; Inspection ; Medical devices ; Medical electronics ; Medical equipment ; Medical instruments ; Microbiological strains ; Nucleotide sequence ; Patients ; PCR ; Penicillin ; Phylogeny ; Risk analysis ; Risk factors ; Sulfonamides ; Trimethoprim ; whole genome sequencing</subject><ispartof>Biofouling (Chur, Switzerland), 2019-02, Vol.35 (2), p.204-216</ispartof><rights>2019 Informa UK Limited, trading as Taylor & Francis Group 2019</rights><rights>2019 Informa UK Limited, trading as Taylor & Francis Group</rights><rights>info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-6eb0aca9b89ebd9f000619af408fad9cf6960b6f0bf82102e9d71b7123f77aa3</citedby><cites>FETCH-LOGICAL-c465t-6eb0aca9b89ebd9f000619af408fad9cf6960b6f0bf82102e9d71b7123f77aa3</cites><orcidid>0000-0001-9351-8535 ; 0000-0002-5604-7145 ; 0000-0002-4787-275X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,776,881,26544</link.rule.ids><linktorsrc>$$Uhttp://hdl.handle.net/10852/76409$$EView_record_in_NORA$$FView_record_in_$$GNORA$$Hfree_for_read</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30950292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikram, Samman</creatorcontrib><creatorcontrib>Heikal, Adam</creatorcontrib><creatorcontrib>Finke, Sarah</creatorcontrib><creatorcontrib>Hofgaard, Antje</creatorcontrib><creatorcontrib>Rehman, Yasir</creatorcontrib><creatorcontrib>Sabri, Anjum Nasim</creatorcontrib><creatorcontrib>Økstad, Ole Andreas</creatorcontrib><title>Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients</title><title>Biofouling (Chur, Switzerland)</title><addtitle>Biofouling</addtitle><description>Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal β-lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs.</description><subject>Ampicillin</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus group</subject><subject>Bacteria</subject><subject>Biofilm</subject><subject>Biofilms</subject><subject>cardiac patients</subject><subject>Catheters</subject><subject>central venous catheters</subject><subject>Chromosomes</subject><subject>Clindamycin</subject><subject>comparative genomics</subject><subject>Contamination</subject><subject>DNA</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Heart</subject><subject>Immunocompromised hosts</subject><subject>Inspection</subject><subject>Medical devices</subject><subject>Medical electronics</subject><subject>Medical equipment</subject><subject>Medical instruments</subject><subject>Microbiological strains</subject><subject>Nucleotide sequence</subject><subject>Patients</subject><subject>PCR</subject><subject>Penicillin</subject><subject>Phylogeny</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Sulfonamides</subject><subject>Trimethoprim</subject><subject>whole genome sequencing</subject><issn>0892-7014</issn><issn>1029-2454</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>3HK</sourceid><recordid>eNp9kUFv1DAQhS1ERZfCTwAiceGSZew4jn2jVEArVeqlZ6yJY6uunHixvaD-exx2lwMHJEtz8Pfmzcwj5A2FLQUJH0EqNgDlWwZUbWkvhZTqGdlQYKplvOfPyWZl2hU6Jy9zfgSAQbD-BTnvQPWVYxvy_TMaH8I-N8YmW8voo_NhblxMMxYfl6Y-Y5eSMDQ_7RJXFMuDLTblJrrmIeadLxh8tlP9SZNH0-yqtGryK3LmMGT7-lgvyP3XL_dX1-3t3bebq8vb1nDRl1bYEdCgGqWy46RcnVRQhY6DdDgp44QSMAoHo5OsbmjVNNBxoKxzw4DYXZB3h7Ym-Vz8opeYUNc79UwPgoOqxIcDsUvxx97momefjQ0BF1tX0owBF1Lxjlf0_T_oY9ynpY5fKUaVYJ1klepPljHnZJ3eJT9jeqq2qzPoU0J6TUgfE6q6t8fu-3G201_VKZIKfDoAfvmTwa-YwqQLPoWYXMLF-Ky7_3v8BrzAn2E</recordid><startdate>20190207</startdate><enddate>20190207</enddate><creator>Ikram, Samman</creator><creator>Heikal, Adam</creator><creator>Finke, Sarah</creator><creator>Hofgaard, Antje</creator><creator>Rehman, Yasir</creator><creator>Sabri, Anjum Nasim</creator><creator>Økstad, Ole Andreas</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TN</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H95</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>3HK</scope><orcidid>https://orcid.org/0000-0001-9351-8535</orcidid><orcidid>https://orcid.org/0000-0002-5604-7145</orcidid><orcidid>https://orcid.org/0000-0002-4787-275X</orcidid></search><sort><creationdate>20190207</creationdate><title>Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients</title><author>Ikram, Samman ; 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Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal β-lactamase genes and a sulphonamide resistant variant of folP. 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| ispartof | Biofouling (Chur, Switzerland), 2019-02, Vol.35 (2), p.204-216 |
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| source | NORA - Norwegian Open Research Archives |
| subjects | Ampicillin Bacillus cereus Bacillus cereus group Bacteria Biofilm Biofilms cardiac patients Catheters central venous catheters Chromosomes Clindamycin comparative genomics Contamination DNA Gene sequencing Genes Genomes Heart Immunocompromised hosts Inspection Medical devices Medical electronics Medical equipment Medical instruments Microbiological strains Nucleotide sequence Patients PCR Penicillin Phylogeny Risk analysis Risk factors Sulfonamides Trimethoprim whole genome sequencing |
| title | Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients |
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