Antibodies raised against aldehyde-fixed antigens improve sensitivity for postembedding electron microscopy

•Antibodies raised against glutaraldehyde-fixed antigen improve immunogold labeling.•Conventional antibodies have poor affinity for aldehyde fixed protein.•Immunolabeling with aldehyde-protein antibodies show low background levels.•Immunization with fixed antigen does not reduce the specificity of t...

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Veröffentlicht in:	Journal of neuroscience methods 2019-04, Vol.317, p.1-10
Hauptverfasser:	Hussain, S., Fredriksen, I., Ringsevjen, H., Kavalali, E.T., Davanger, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Electron microscopy Fixation Formaldehyde Glutaraldehyde
Online-Zugang:	Volltext
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