Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Here the relation between digestion of proteins by trypsin covalently bound to paper and trypsin in‐solution is investigated. The trypsin acting on paper is covalently bound. A trypsin concentration of 0.5% (w/v) results in the highest digestion activity of all concentrations tested. Additionally, i...

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Veröffentlicht in:	Separation science plus 2022-05, Vol.5 (5), p.171-183
Hauptverfasser:	Nguyen, Minh Thao, Halvorsen, Trine Grønhaug, Thiede, Bernd, Reubsaet, Léon
Format:	Artikel
Sprache:	eng
Schlagworte:	dried blood spot microsampling pre‐lab digestion protein analysis smart sampling
Online-Zugang:	Volltext
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